Presence of avipoxvirus DNA in avian dermal squamous cell carcinoma

Fallavena, Luiz Cesar Bello; Canal, Cláudio Wageck; Salle, Carlos Tadeu Pippi; Moraes, H. L. S.; Rocha, Silvio L.S.; Pereira, Rafael Mendes; Silva, Ari B. da

doi:10.1080/03079450220136558

Cited by 35 publications

(24 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Back et al (1995) reports an outbreak of Fowlpox in broiler chickens and the diagnostic was performed by histopathology and virus isolation. Fallavena et al (2002) reports the presence of FWPV in Dermal Squamous Cell Carcinoma (DSCC) also in broiler chickens using a nested PCR. Silva et al (2009) reports the presence of Fowlpox in backyard chickens by clinical signs and confirming the diagnosis by histopathology.…”

Section: Discussionmentioning

confidence: 99%

First phylogenetic analysis of Avipoxvirus (APV) in Brazil

et al. 2016

View full text Add to dashboard Cite

RESUMO.-[Primeira análise filogenética de Avipoxvirus (APV) no Brasil.] Este trabalho representa a primeira aná-lise filogenética de Poxvirus aviário detectado em perus no Brasil. Os distúrbios clínicos relacionados com bouba aviá-ria aqui descritos ocorreram em um sistema de alojamento de perus. As aves apresentaram lesões características de varíola observadas no pescoço, pálpebras e bico das aves. Quatro perus com sinais característicos foram escolhidos aleatoriamente, sacrificados e submetidos à autópsia. This study represents the first phylogenetic analysis of avian poxvirus recovered from turkeys in Brazil. The clinical disorders related to fowlpox herein described occurred in a turkey housing system. The birds displaying characteristic pox lesions which were observed on the neck, eyelids and beak of the turkeys. Four affected turkeys were randomly chosen, euthanized and necropsied. Tissues samples were submitted for histopathological analysis and total DNA was further extracted, amplified by conventional PCR, sequenced and phylogenetically analyzed. Avian poxviruses specific PCR was performed based on P4b core protein gene sequence. The histological analysis revealed dermal inflammatory process, granulation tissue, hyperplasia of epithelial cells and inclusion bodies. The P4b gene was detected in all samples. Sequencing revealed a 100% nucleotide and amino acid sequence identity among the samples, and the sequences were deposited in GenBank ® . The four Avian poxviruses fragments sequenced in this study clustered along the A1 clade of avipoxviruses, and were classified as Avipoxvirus (APV). Additional studies, such as virus isolation, PCR and sequencing includinga large number of specimens from the Brazilian turkey production must be conducted due to the hazardous risk that poxvirus infections may cause to the Brazilian poultry production scenario, given that Brazil's turkey production attracts attention due to its economic importance worldwide. Our findings point to the need to identify the prevalence of APV in Brazilian turkey production, to perform risk assessment studies and continued surveillance of APV infections in both wild and commercial avian species. . Estudos adicionais, como isolamento viral, PCR e sequenciamento, incluindo um grande número de perus da produção brasileira devem ser conduzidos devido ao grave risco que a infecção por poxvírus pode causar ao cenário de produção avícola brasileira, tendo em vista que a produção brasileira de perus atrai atenção devido a sua importância mundial. Nossos resultados apontam para a necessidade de identificar a prevalência da APV na produção de peru no Brasil, para realizar estudos de avaliação de risco e continuada monitoração de infecções por APV nas espécies de aves comerciais e silvestres.

show abstract

Section: Discussionmentioning

confidence: 99%

First phylogenetic analysis of Avipoxvirus (APV) in Brazil

et al. 2016

View full text Add to dashboard Cite

show abstract

“…DSCC is generally encountered in broiler chickens during slaughtering (10) and is differed from true squamous cell carcinoma by not showing malignancy and regression with the time progress (5). The etiology of naturally occurring DSCC is not known but experimentally repeated application of methylcholanthrene and some viral agents such as Avipox and Avian Leucosis Viruses are thought to play a role (4,5). Although the pathogenesis is not clear; the lesions are considered to have started from hyperplasic feather follicles and infiltration of the peripheral follicles into the dermis or formation of small follicular cysts whose walls are made of kerotinocytes.…”

Section: Muhabbet Kuşlarında Dermal Yassı Hücreli Kanser (Keratoacantmentioning

confidence: 99%

Dermal squamous cell carcinoma (avian keratoacanthoma) in parakeets

Ziynet;OZTURK¹

2010

Ankara Üniversitesi Veteriner Fakültesi Dergisi

View full text Add to dashboard Cite

Summary:Tumor suspected operation materials obtained from eight parakeets are evaluated clinically, pathologically and immunohistochemically. The age of the parakeets varied from seven months to nine years old. Four were male, one was a female and the sexes of the rest three were not identified. Six of the operation materials were taken from the above the tail while the other two from beneath the wings. Evaluated tumors were observed to be hard in consistency and whitish color on cut surface. At histopathological examination; it is seen that hyperchromatic nucleated, big, ovoid shaped, pale eosinophilic cytoplasmated cells had formed groups in dermis. Keratinized horn pearl formations are identified in the centers of most of these cell groups. Within the horn pearl formations hyperkeratotic, parakeratotic changes and calcifications are found. In some cases inflammation areas consisting of heterophil leukocytes, lymphocytes and plasma cells in the dermis are observed. Also in histopathological examination of one case, tubercle formations made of coagulation necrosis in the center surrounded by heterophil leukocytes, macrophages, epithelioid cells, lymphocytes and foreign body giant cells are found as well as tumoral formations. Groups of cudgel shaped, red colored bacteria are found in Ziehl-Neelsen staining of the sections. Tissues are stained for proliferating cell nuclear antigen (PCNA) and p53 proteins immunohistochemically in order to interpret mitotic activity and malignancy of the tumor. TUNEL method is applied to the tissues to evaluate apoptosis.Key words: Dermal squamous cell carcinoma, histopathology, immunohistochemistry, keratoacanthoma, parakeet. Muhabbet kuşlarında dermal yassı hücreli kanser (keratoacanthoma)Özet: Tümör şüpheli sekiz muhabbet kuşundan alınmış operasyon materyalleri klinik, patolojik ve immunhistokimyasal olarak değerlendirildi. İncelenen muhabbet kuşları 7ay-9 yaş arasındaydı ve dördü erkek, biri dişi iken üç tanesinin cinsiyeti bildirilmemişti. Operasyon materyallerinin altısı kuyruk üzerinden ikisi ise kanat altından alınmıştı. İncelenen tümörlerin sert kıvamlı ve kesit yüzlerinin ise beyazımtırak renkte olduğu gözlendi. Tümöral kitlelerin histopatolojik incelenmesinde; dermiste hiperkromatik çekirdekli, büyük, ovoid şekilli, solgun eozinofilik sitoplazmalı hücrelerin gruplar oluşturduğu dikkati çekti. Bu hücre gruplarının bir çoğunun orta kısımlarında keratinize glob korne yapıları belirlendi. Glob korne yapıları içerisinde hiperkeratotik ve parakeratotik değişiklikler ile kalsifikasyonlara rastlandı. Olguların bazılarında dermiste nötrofil lökosit, lenfosit ve plazma hücrelerinden oluşan yangısal alanlar görüldü. Ayrıca bir olguda, saptanan tümörden başka aynı hayvandan alınan kitlelerin histopatolojik incelenmesinde ise; ortada koagulasyon nekrozu alanının etrafında heterofil lökositler, histiyositler, epiteloid hücreler, lenfositler ve yabancı cisim dev hücrelerinin oluşturduğu tüberkül yapısına rastlandı. Yapılan Ziehl-Neelsen boyamasında kırmızı renkli, çomak şekilli bakte...

show abstract

“…Genomic DNA sequences of various sizes can be amplified by the polymerase chain reaction (PCR) using specific primers (Fallavena et al, 2002;Lee & Lee, 1997). This technique is useful when there is only an extremely small amount of viral DNA in the sample.…”

Section: Ii)mentioning

confidence: 99%