Presence of chromogranin A, B and C in bovine endocrine and nervous tissues: A comparative immunohistochemical study

Abstract

Antisera against chromogranin A, B and C were used to study the distribution of these acidic proteins in bovine endocrine and nervous tissues. The three chromogranins occur together in several endocrine organs (adrenal medulla, anterior pituitary, endocrine pancreas) and in sympathetic ganglion cells. In the posterior pituitary, only chromogranin C and in the intermediate lobe only A and C are found. The parathyroid gland contains only A, and enterochromaffin cells are immunoreactive for A and B. Cells of the thyroid gland and some cells of the anterior pituitary apparently do not contain any chromogranins. It is concluded that the three chromogranins are not always stored together and that they are not present in all endocrine cells. This distinct localization of the chromogranins indicates some special, although still undiscovered, function for these proteins.

Pituitary-like proopiomelanocortin transcripts in human Leydig cell tumors.

“…The presence of secretory granules and their biochemical markers, the chromogranins, have been stressed as the specific hallmark of neuro-endocrine or peptide hormone secreting cells to discriminate them from other endocrine cells which produce steroid or thyroid hormones (53,54). These secretory granules are also associated with a specific secretory mechanism called the regulatory pathway (55).…”

Bovine Posterior Pituitary: Presence of p65 (Synaptotagmin), PC1 PC2 and Secretoneurin in Large Dense Core Vesicles

“…Since in these fractions some ACTH is also found, the most likely explanation is that small amounts of the inter mediate lobe are dissected out together with the posterior one. The intermediate lobe contains a high concentration of ACTH [49] and also secretogranin II [50]. Whereas secretogranin II in the LDV of posterior pituitary lobe appears completely processed, the processing in the pitu itary stalk is still incomplete since high molecular immunoreactive material representing intact secretogranin II is found.…”

The Neuroendocrine System of the Gut

“…Glieentin and PYY coexist in a major population of endocrine cells in the distal intestine (16, 17). It may also be mentioned in this context that certain large molecular weight proteins, such as chromogranins, have been found to be universally distributed in granules of neuroendocrine cells including those of the gut (18)(19)(20)(21).…”