Presence of Conjugated Catecholamines in Rat Brain: A New Method of Analysis of Catecholamine Sulfates

Buu, Nguyen T.; Duhaime, Johanne; Savard, Christopher E.; Truong, Linda; Küchel, Otto

doi:10.1111/j.1471-4159.1981.tb01655.x

Cited by 48 publications

(27 citation statements)

References 11 publications

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“…In rats and especially dogs, but not in guinea pigs, there was substantial sulfatation observed after oral DA application which did not occur after intravenous application [138]. There are even differences in respect to different brain areas with higher degree of sulfatation in the hypothalamus and hippocampus, and a lower percentage in the striatum [136]. …”

Section: Dopamine Degradationmentioning

confidence: 99%

Complexity of dopamine metabolism

2013

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Parkinson’s disease (PD) coincides with a dramatic loss of dopaminergic neurons within the substantia nigra. A key player in the loss of dopaminergic neurons is oxidative stress. Dopamine (DA) metabolism itself is strongly linked to oxidative stress as its degradation generates reactive oxygen species (ROS) and DA oxidation can lead to endogenous neurotoxins whereas some DA derivatives show antioxidative effects. Therefore, DA metabolism is of special importance for neuronal redox-homeostasis and viability.In this review we highlight different aspects of dopamine metabolism in the context of PD and neurodegeneration. Since most reviews focus only on single aspects of the DA system, we will give a broader overview by looking at DA biosynthesis, sequestration, degradation and oxidation chemistry at the metabolic level, as well as at the transcriptional, translational and posttranslational regulation of all enzymes involved. This is followed by a short overview of cellular models currently used in PD research. Finally, we will address the topic from a medical point of view which directly aims to encounter PD.

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Section: Dopamine Degradationmentioning

confidence: 99%

Complexity of dopamine metabolism

2013

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“…2B). Such a ratio was also found in rat brain, where free DA is significantly predominant over DA-S (Buu et al, 1981a). We also detected a large quantity of conjugated DA (∼2 ng/tick) compared to free DA (∼5pg/tick) in the haemolymph of ticks (Fig.…”

Section: Discussionmentioning

confidence: 54%

Autocrine/paracrine dopamine in the salivary glands of the blacklegged tick Ixodes scapularis

Koči

Šimo

Park

2014

Journal of Insect Physiology

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Dopamine (DA) is known to be the most potent activator of tick salivary secretion, which is an essential component of successful tick feeding. We examined the quantitative changes of catecholamines using a method coupling high-pressure liquid chromatography with electrochemical detection (HPLC-ECD). We also investigated the levels of catecholamines conjugated to other molecules utilising appropriate methods to hydrolyse the conjugates. Three different biological samples, salivary glands, synganglia, ovaries and haemolymph were compared, and the largest quantity of DA was detected in salivary gland extracts (up to ∼100 pg/tick), supporting the hypothesis that autocrine/paracrine dopamine activates salivary secretion. Quantitative changes of catecholamines in the salivary glands over the entire blood feeding duration were examined. The amount of dopamine in the salivary glands increased until the day 5 of feeding, at which the rapid engorgement phase began. We also detected a small but significant amount of norepinephrine in the salivary glands. Interestingly, saliva collected after induction of salivary secretion by the cholinergic agonist pilocarpine contained a large amount of DA sulphate with a trace amount of DA, suggesting a potential biological role of DA sulphate in tick saliva.

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“…The concentration of probenecid in the rat brain 1 h after intraperitoneal injection of 200 mg/kg, a dose com monly used in turnover studies [Neff et al, 1967;Neff, 1972, 1973a], has been reported to be 50 pg/g tissue (approxi mately 0.17 mM) [Wijk et al, 1979], Under these conditions, we would expect PST ac tivity to be unaffected assuming that the concentration of probenecid in the whole rat brain equals that in the striatum. However, DA concentration in many other regions of the brain (such as hypothalamus, hippocam pus, cortex, medulla, cerebellum) is below 1.5 pM [Shellenberger and Gordon, 1971;Buu et al, 1981]. It is likely that PST activity could be affected by probenecid treatment in these regions.…”

Section: Resultsmentioning

confidence: 99%

“…Both DAS and PST are present in central and periph eral tissues [Foldes and Meek, 1974;Buu et al, 1981;Rein et al, 1982;Karoum, 1983;Yoneda et al, 1983]. Many monoamine acidic metabolites have been shown to be removed from the brain or cerebrospinal fluid by a probenecid-sensitive active trans port mechanism.…”

Section: Introductionmentioning

confidence: 99%

Effect of Probenecid on Rat Brain Phenolsulfotransferase Activity Using Dopamine as Substrate

Kao¹,

Elchisak²

1987

Pharmacology

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Dopamine sulfate (DAS) is formed by the action of phenolsulfotransferase (PST) on dopamine (DA). Since probenecid is often used to investigate the transport of monoamine acidic metabolites such as DAS from the brain, we investigated the effect of probenecid on rat brain PST activity utilizing DA as substrate. In the presence of 30 or 90 μM DA, probenecid had either no effect or a dose-related inhibitory effect on PST activity. PST activity increased at low probenecid concentrations, but decreased at high probenecid concentrations when 360 μM DA was utilized. Several conditions were found where PST activity was not affected by probenecid.

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Presence of Conjugated Catecholamines in Rat Brain: A New Method of Analysis of Catecholamine Sulfates

Cited by 48 publications

References 11 publications

Complexity of dopamine metabolism

Complexity of dopamine metabolism

Autocrine/paracrine dopamine in the salivary glands of the blacklegged tick Ixodes scapularis

Effect of Probenecid on Rat Brain Phenolsulfotransferase Activity Using Dopamine as Substrate

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