Presence of Estrogens and Estrogen Receptor-like Proteins in Solanum glaucophyllum

Milanesi, Lorena Magdalena; Monje, Paula V.; Boland, Ricardo

doi:10.1006/bbrc.2001.6079

Cited by 29 publications

(32 citation statements)

References 25 publications

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“…In fact, before the genome era the same situation was found for NR and many reports claimed for the detection of steroid receptors in a wide variety of non-metazoans organisms such as plants or fungi (see Agarwal et al, 1994, Milanesi et al, 2001, Milanesi and Boland, 2006. Despite the fact that, as in the above mentioned studies, all the controls were correct, all these studies displayed artefacts: there is no gene related to steroid receptors outside metazoans (Escriva et al, 1997, Escriva et al, 2004.…”

Section: Detectionmentioning

confidence: 62%

The evolution of the ligand/receptor couple: A long road from comparative endocrinology to comparative genomics

Markov

Paris

Bertrand

et al. 2008

Molecular and Cellular Endocrinology

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The evolution of the ligand/receptor couple: a long road from comparative endocrinology to comparative genomics. Molecular and Cellular Endocrinology, Elsevier, 2008, 293 (1-2) This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.Page 1 of 32A c c e p t e d M a n u s c r i p t

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Section: Detectionmentioning

confidence: 62%

The evolution of the ligand/receptor couple: A long road from comparative endocrinology to comparative genomics

Markov

Paris

Bertrand

et al. 2008

Molecular and Cellular Endocrinology

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“…These plant compounds have got structural similarities with mammalian 17β-estradiol (E2) and are capable of binding to estrogen receptors (ERs) [5][6][7]. Even though phytoestrogens can activate ERs, their effects are approximately thousand folds weaker than E2 [8].…”

Section: Introductionmentioning

confidence: 99%

“…As mentioned, the synthesis of molecules structurally and functionally related to the mammalian steroid hormones by plants, has been reported [5,6,10,11]. However, their physiological role in plants is controversial.…”

Section: Introductionmentioning

confidence: 99%

“…(Solanum malacoxylon Send. ), Lycopersicon esculentum (Mill) and Nicotiana glauca (Graham) by competition assays using specific polyclonal antibodies against the respective mammalian steroid hormones [5,6,12]. Binding experiments with [ 3 H]17β-estradiol in the presence or absence of an excess of the unlabelled steroid showed that the Solanaceae organs contain estrogen binding sites.…”

Section: Introductionmentioning

confidence: 99%

“…The protein nature of these sites was clearly indicated by their sensitivity to trypsin degradation. Likewise, lipid crude extracts from Solanaceae were able to compete with [ 3 H]17β-estradiol for binding to the estrogen receptor (ER) from breast cancer MCF-7 cells as well as with estrogen binding sites present in this plant species [5,6,12].…”

Section: Introductionmentioning

confidence: 99%

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EXTRACTS FROM <em>Nicotiana glauca</em> INDUCE APOPTOSIS THROUGH CASPASES IN SKELETAL MUSCLE CELLS

Lincor

Musso

Vasconsuelo

et al. 2017

Proceedings of the 21st International Electronic Conference on Synthetic Organic Chemistry

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Phytoestrogens are plant compounds which have generated considerable interests. A litany of health benefits including a lowered risk of osteoporosis, breast cancer, and menopausal symptoms, are frequently attributed to phytoestrogens but without the knowledge of their side effects. We investigate the effects of lipid extracts from the Solanaceae Nicotiana glauca on skeletal muscle cells, in relation to apoptosis. Opposite to the effects of 17β-estradiol, the crude extract from N. glauca and its sub-extracts induced apoptosis in C2C12 cells. This apoptotic action involved caspase 3/7 activation. These data suggest that the traditional use of this medicinal plant could affect the skeletal muscle homeostasis.

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Semicontinuous bioreactor production of a recombinant human therapeutic protein using a chemically inducible viral amplicon expression system in transgenic plant cell suspension cultures

Huang

Plesha

McDonald

2010

Biotech & Bioengineering

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Plant cell culture is an alternative for the production of recombinant human therapeutic proteins because of improved product safety, lower production cost, and capability for eukaryotic post-translational modification. In this study, bioreactor production of recombinant human alpha-1-antitrypsin (rAAT) glycoprotein using a chemically inducible Cucumber mosaic virus (CMV) viral amplicon expression system in transgenic Nicotiana benthamiana cell culture is presented. Optimization of a chemically inducible plant cell culture requires evaluation of effects of timing of induction (TOI) and concentration of inducer (COI) on protein productivity and protein quality (biological functionality). To determine the optimal TOI, the oxygen uptake rate (OUR) of the plant cell culture was chosen as a physiological indicator for inducing maximum rAAT expression. Effects of COI on rAAT production were investigated using a semicontinuous culture, which enables the distinction between effects of growth rate and effects of inducer concentration. An optimized semicontinuous bioreactor operation was further proposed to maximize the recombinant protein production. The results demonstrated that the transgenic plant cells, transformed with the inducible viral amplicon expression system, maintain higher OUR and exhibit lower extracellular protease activity and lower total phenolics concentration in the optimized semicontinuous bioreactor process than in a traditional batch bioreactor operation, resulting in a 25-fold increase in extracellular functional rAAT (603 microg/L) and a higher ratio of functional rAAT to total rAAT (85-90%). Surprisingly, sustained rAAT production and steady state, long-term bioreactor operation is possible following chemical induction and establishment of the viral amplicons.

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Presence of Estrogens and Estrogen Receptor-like Proteins in Solanum glaucophyllum

Cited by 29 publications

References 25 publications

The evolution of the ligand/receptor couple: A long road from comparative endocrinology to comparative genomics

The evolution of the ligand/receptor couple: A long road from comparative endocrinology to comparative genomics

EXTRACTS FROM <em>Nicotiana glauca</em> INDUCE APOPTOSIS THROUGH CASPASES IN SKELETAL MUSCLE CELLS

Semicontinuous bioreactor production of a recombinant human therapeutic protein using a chemically inducible viral amplicon expression system in transgenic plant cell suspension cultures

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