Presence of HLA-D determinants on human macrophages

Characterisation of macrophages infiltrating human mammary carcinomas

“…Macrophages are known to bear Fc(IgG) receptors (Berken & Benacerraf, 1966;Nelson, 1981) and to express HLA-DR antigens (Hirchberg et al, 1976). Morphologically, the rosetting cells in the present study resembled macrophages and a variable percentage contained phagocytosed indicator red blood cells.…”

Stimulation of Human Lymphocytes by Allogenic Macrophages in vitro

“…Macrophages from HLA-D homozygous donors stimulated only weakly responding lymphocytes which shared the HLA-D determinant with them. We have previously reported (Hirschberg et al 1976) that stimulation by macrophages from donors carrying HLA-Dw2 is specifically inhibited by an antiserum apparently recognizing Dw2 (or a very closely associated antigen). Thus, the strongest stimulating antigens on human macrophages derived from peripheral blood adherent monocytes appear to be determinants governed by the HLA-D locus, and are therefore identical to those which are mainly responsible for stimulation by lymphocytes, skin cells (Hirschberg & Thorsby 1975) and sperms (Halim & Festenstein 1975).…”

“…These determinants have previously been shown to be expressed on lymphocytes, mainly B lymphocytes (Lohrmann e t al. 1974), epithelial cells (Hirschberg & Thorsby 1976) and sperm cells (Halim & Festenstein 1975). The HLA-D determinants seem t o be analogous t o some of the la antigens of mice (For references see .…”

“…For this reason we have investigated further the allo-activating capacity of human macrophages, and whether this capacity is due to their expression of HLA-D. A brief communication of some of our results has appeared elsewhere (Hirschberg et al 1976).…”

Dynamics of the Macrophage Plasma Membrance

“…Human monocyte C3 receptor activity was inhibited by corticosteroids (226). DETERMINANTS Cell surface la-like antigens associated with the HLA D region have been observed on human macrophages (2,113). Human peripheral blood monocytes in culture for 8 days were capable of stimulat ing allogeneic lymphocytes in an in vitro mixed lymphocyte-macrophage culture by determinants coded for by the HLA-D locus (113).…”