Characterisation of macrophages infiltrating human mammary carcinomas

Steele, Robert; Brown, Monica; Eremin, O.

doi:10.1038/bjc.1985.20

Cited by 25 publications

(12 citation statements)

References 19 publications

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“…The finding of large numbers of macrophages within benign and malignant breast tissue is in accordance with recent observations by Lwin et al (1985), Ferguson (1985), and Steele et al (1985). We also observed a significant number of intraepithelial macrophages in benign breast tissue as well as within tumour epithelium (Lwin et al, 1985).…”

Section: Discussionsupporting

confidence: 93%

“…Among tumours where such a response is of particular interest is human mammary carcinoma which has the potential for metastasis at a relatively early stage of the disease and in which metastases may appear many years after apparently adequate local treatment. Although lymphocytes are prominent in the inflammatory infiltrate, macrophages are also present, often in considerable numbers (McBride, 1986;Steele et al 1985;Lwin, et al 1986). It has been suggested that the presence of macrophages may independently influence the outcome by affecting the metastatic potential of tumours (Lauder et al, 1977) and there is evidence from experimental animal tumours that this is so (Fidler & Poste, 1982;Eccles & Alexander, 1974; Wood & Gillespie, 1975).…”

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confidence: 99%

“…Enzymes such as acid phosphatase and alphanaphthyl acetate esterase (ANAE), demonstrated by histochemical techniques, are also not specific and may be found in a variety of tumours including, in the case of ANAE, mammary carcinoma (Tubbs et al, 1979 (Steele et al, 1985;Lwin et al, 1985). Among the drawbacks associated with the use of such antibodies, however, is failure to detect all tissue macrophages as in the case of UCHM-1 (Hogg et al, 1984) used in the study of Lwin et al (1985) (Kraft et al, 1981), the antibody used in the study of Steele et al (1985).…”

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confidence: 99%

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Macrophages in human breast disease: a quantitative immunohistochemical study

Kelly

Davison²,

Bliss³

et al. 1988

Br J Cancer

154

105

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We describe a quantitative histological study of 34 breast biopsies using a marker for human macrophages, the monoclonal antibody EBM/11. Seventeen of the biopsies were of malignant tumours. Both benign and malignant breast tissue contained large numbers of macrophages with significantly higher numbers occurring in the malignant group. An analysis was made of macrophage counts according to stage, grade and prognostic index of the malignant tumours. There was no correlation between macrophage numbers and any of these parameters in malignant breast tumours. We discuss the possible reasons why some earlier studies (using other markers such as lysozyme), have shown an apparently insignificant number of intratumoral macrophages. Images Figure 1 Figure 2

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Section: Discussionsupporting

confidence: 93%

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confidence: 99%

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confidence: 99%

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Macrophages in human breast disease: a quantitative immunohistochemical study

Kelly

Davison²,

Bliss³

et al. 1988

Br J Cancer

154

105

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“…cytosol from the presumptive ER-negative infiltrating cells diluting the possibly ER-positive tumour cell cytosol) because the maximal Leu7-positive cell infiltration was sparse relative to that of other lymphoid cells. Dilution may, however, explain the observed significant inverse correlation between ER status and macrophage infiltration (Steele et al, 1986); macrophages may constitute 20-60% of the total cell population in breast cancers (Steele et al, 1985). Any association between ER negativity and Leu7-positive cell infiltration is therefore probably superimposed on this dilutional effect of macrophages and other infiltrating cells.…”

Section: Discussionmentioning

confidence: 99%

Immunophenotype of the lymphoid cell infiltrates in breast carcinomas of low oestrogen receptor content

Underwood

Giri²,

Rooney³

et al. 1987

Br J Cancer

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Summary Several studies of breast tumour histology and oestrogen receptor (ER) contents report an inverse correlation between the density of the stromal lymphoid cell infiltrate and the presence or concentration of ER. Using monoclonal antibodies to lymphoid cell surface markers we have examined the immunophenotype of the infiltrating lymphoid cells and related this to the tumour ER content. There is a significant inverse correlation between the presence of Leu7-positive cells, which includes a subpopulation of natural killer (NK) cells, and tumour ER content; this is possibly superimposed on the dilutional effect of macrophages and other infiltrating cells.

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“…This may be caused by a different drug concentration obtained in vivo compared with the ideal in vitro situation and by the fact that in vivo tumours also contain nontumour cells such as endothelial cells, macrophages and lymphocytes, 28,29 which lack the suicide gene transfected to the Morris hepatoma cells. These nontumour cells contribute to the signal obtained in metabolic studies as shown by MR spectroscopy.…”

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confidence: 99%

Uncoupling of 2-fluoro-2-deoxyglucose transport and phosphorylation in rat hepatoma during gene therapy with HSV thymidine kinase

et al. 1998

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This animal study investigates the application of positron poration into the tumour DNA was determined after i.v. emission tomography (PET) with tracers of tumour metabadministration of 3 H-thymidine. An uncoupling of FDG olism for monitoring suicide gene therapy with herpes simtransport and phosphorylation was found with enhanced K 1 plex virus thymidine kinase (HSVtk). After transplantation and k 2 values and a normal k 3 after 2 days of GCV treatof HSVtk-expressing Morris hepatoma cells into ACI rats, ment. The increase in FDG transport normalized after 4 dynamic PET measurements of 18 F-labeled 2-fluoro-2-days whereas the phosphorylation rate k 3 increased. deoxyglucose (FDG) uptake were performed in animals 2 Quantification using the metabolic rate or the SUV showed days (n = 7) and 4 days (n = 5) after the onset of therapy congruent but less sensitive results compared with the with 100 mg ganciclovir (GCV)/kg body weight as well as modeling approach. The thymidine incorporation into the after administration of sodium chloride (n = 8). The arterial DNA of the tumours declined to 10.5% of the controls after FDG plasma concentration was measured dynamically in 4 days of GCV treatment. The data indicate that PET an extracorporeal loop and the rate constants for FDG with 18 FDG and 11 C-thymidine may be applied for monitortransport (K 1 , k 2 ) and FDG phosphorylation (k 3 ) were caling of gene therapy with the HSVtk/GCV suicide system. culated using a three-compartment model modified for hetIncreased transport rates are evidence of stress reactions erogeneous tissues. Also, quantification using the metaearly after therapy. The measurement of thymidine incorbolic rate of FDG turnover and the standardized uptake poration into the tumour DNA can be used as an indicator value (SUV) was done. Furthermore, the thymidine incorof therapy efficacy.

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Characterisation of macrophages infiltrating human mammary carcinomas

Cited by 25 publications

References 19 publications

Macrophages in human breast disease: a quantitative immunohistochemical study

Macrophages in human breast disease: a quantitative immunohistochemical study

Immunophenotype of the lymphoid cell infiltrates in breast carcinomas of low oestrogen receptor content

Uncoupling of 2-fluoro-2-deoxyglucose transport and phosphorylation in rat hepatoma during gene therapy with HSV thymidine kinase

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