Presence of HPV DNA in extracellular vesicles from HeLa cells and cervical samples

Mata-Rocha, Minerva; Rodríguez-Hernández, Ruth Monserrat; Chávez-Olmos, Pedro; Garrido, Efraín; Robles-Vázquez, Conrado; Aguilar-Ruiz, Sergio Roberto; Torres-Aguilar, Honorio; González-Torres, Carolina; Gaytán-Cervantes, Javier; Mejı́a-Aranguré, Juan Manuel; Romero-Tlalolini, María de los Ángeles

doi:10.1016/j.eimc.2019.06.011

Cited by 29 publications

(26 citation statements)

References 25 publications

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“…For example , Meckes Jr et al (2010) identified that ERK and PI3K/AKT signaling pathways can be activated if the recipient cells were exposed to exosomes containing major EBV oncogene LMP1. Besides, Human papillomavirus (HPV) is considered a risk factor for OSCC, and its DNA has been found in exosomes from HeLa cells (Mata-Rocha et al, 2020). There are some evidence demonstrating that HPV DNA in exosomes participated in the process of cancer.…”

Section: Dnamentioning

confidence: 99%

Exosomal cargoes in OSCC: current findings and potential functions

Zhao

Zhang

Liu

et al. 2020

PeerJ

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Oral squamous cell carcinoma (OSCC) is the most prevalent malignancy in head and neck cancer, with high recurrence and mortality. Early diagnosis and efficient therapeutic strategies are vital for the treatment of OSCC patients. Exosomes can be isolated from a broad range of different cell types, implicating them as important factors in the regulation of human physiological and pathological processes. Due to their abundant cargo including proteins, lipids, and nucleic acids, exosomes have played a valuable diagnostic and therapeutic role across multiple diseases, including cancer. In this review, we summarize recent findings concerning the content within and participation of exosomes relating to OSCC and their roles in tumorigenesis, proliferation, migration, invasion, metastasis, and chemoresistance. We conclude this review by looking ahead to their potential utility in providing new methods for treating OSCC to inspire further research in this field.

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Section: Dnamentioning

confidence: 99%

Exosomal cargoes in OSCC: current findings and potential functions

Zhao

Zhang

Liu

et al. 2020

PeerJ

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“…With the high mortality rate of cervical cancers, more information is needed regarding detection and treatment of the diseased. In this regard, several investigators have begun to examine extracellular vesicles (EVs) and their role in disease protection [2], as disease biomarkers [3][4][5] and/or as a cargo/vaccine agents for cervical cancer [6][7][8][9][10][11][12][13][14][15][16][17][18].…”

Section: Introductionmentioning

confidence: 99%

Alcohol Exposure Impacts the Composition of HeLa-Derived Extracellular Vesicles

et al. 2019

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Extracellular vesicles are nanosized vesicles that are under intense investigation for their role in intercellular communication. Extracellular vesicles have begun to be examined for their role in disease protection and their role as disease biomarkers and/or vaccine agents. The goal of this study was to investigate the effects of alcohol exposure on the biogenesis and composition of extracellular vesicles derived from the cervical cancer line, HeLa. The HeLa cells were cultured in exosome-free media and were either mock-treated (control) or treated with 50 mM or 100 mM of alcohol for 24 h and 48 h. Our results demonstrated that alcohol significantly impacts HeLa cell viability and exosome biogenesis/composition. Importantly, our studies demonstrate the critical role of alcohol on HeLa cells, as well as HeLa-derived extracellular vesicle biogenesis and composition. Specifically, these results indicate that alcohol alters extracellular vesicles’ packaging of heat shock proteins and apoptotic proteins. Extracellular vesicles serve as communicators for HeLa cells, as well as biomarkers for the initiation and progression of disease.

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“…Exosomes from various sources were found to carry fragments of genomic, mitochondrial and viral DNA [102][103][104]. Exosomes derived from normal neural stem cells were compared to a neuroblastoma cell line or glioblastoma cells, were shown to package DNA harboring mutations in the form of SNPs in the SOX2 gene or insertions in the NANOGP8 gene [105,106] These differences can be harnessed to establish exosome based diagnostic biomarkers for hard-to-reach cancers.…”

Section: Dnamentioning

confidence: 99%

The Multifunctionality of Exosomes; from the Garbage Bin of the Cell to a Next Generation Gene and Cellular Therapy

Shrivastava

Morris

2021

Genes

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Exosomes are packaged with a variety of cellular cargo including RNA, DNA, lipids and proteins. For several decades now there has been ongoing debate as to what extent exosomes are the garbage bin of the cell or if these entities function as a distributer of cellular cargo which acts in a meaningful mechanistic way on target cells. Are the contents of exosomes unwanted excess cellular produce or are they selective nucleic acid packaged nanoparticles used to communicate in a paracrine fashion? Overexpressed RNAs and fragments of DNA have been shown to collect into exosomes which are jettisoned from cells in response to particular stimuli to maintain homeostasis suggesting exosomes are functional trash bins of the cell. Other studies however have deciphered selective packaging of particular nucleic acids into exosomes. Nucleic acids packaged into exosomes are increasingly reported to exert transcriptional control on recipient cells, supporting the notion that exosomes may provide a role in signaling and intracellular communication. We survey the literature and conclude that exosomes are multifunctional entities, with a plethora of roles that can each be taken advantage to functionally modulate cells. We also note that the potential utility of developing exosomes as a next generation genetic therapy may in future transform cellular therapies. We also depict three models of methodologies which can be adopted by researchers intending to package nucleic acid in exosomes for developing gene and cell therapy.

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Presence of HPV DNA in extracellular vesicles from HeLa cells and cervical samples

Cited by 29 publications

References 25 publications

Exosomal cargoes in OSCC: current findings and potential functions

Exosomal cargoes in OSCC: current findings and potential functions

Alcohol Exposure Impacts the Composition of HeLa-Derived Extracellular Vesicles

The Multifunctionality of Exosomes; from the Garbage Bin of the Cell to a Next Generation Gene and Cellular Therapy

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