Presence of Mycoplasma haemofelis, Mycoplasma haemominutum and piroplasmids in cats from southern Europe: a molecular study

Frequency and hematological alterations of different hemoplasma infections with retrovirusis co-infections in domestic cats from Brazil

“…The frequency of hemoplasma infections observed in both groups wasn't different, but higher than the prevalence previously reported in North America (19.7 to 22.7%) (Gary et al 2006) and similar to the rates detected in European cats (28 to 30%) (Criado-Fornelio et al 2003). However, the higher frequency of Candidatus Mycoplasma haemominutum (47%) over Mycoplasma haemofelis (35.3%) in cats from urban areas was the opposite found in Great Britain, where 66% of hemoplasma positive cats were infected by Mycoplasma haemofelis and 33% by Candidatus Mycoplasma haemominutum.…”

“…These observations are also commonly noticed in hemoplasma infected cats, suggesting the low pathogenicity of some hemoplasma infections (Willi et al 2006). As G1 animals had a higher infection rate of Mycoplasma haemofelis (Table 1), known to be more pathogenic (Criado--Fornelio et al, 2003), the reduced values observed in the erythrograms from these cats may be caused by hemoparasite infections. Also, 37.2% of G1 cats were FeLV positive, in opposition to G2, where none of the cats had retroviruses.…”

“…However, the higher frequency of Candidatus Mycoplasma haemominutum (47%) over Mycoplasma haemofelis (35.3%) in cats from urban areas was the opposite found in Great Britain, where 66% of hemoplasma positive cats were infected by Mycoplasma haemofelis and 33% by Candidatus Mycoplasma haemominutum. This difference could be associated with different forms of transmission other than vectors and the weather conditions associated with it (Criado-Fornelio et al 2003). It is postulated that transmissions can also occur via contact with contaminated saliva, feces and/or urine.…”

“…infection as previously described (Criado-Fornelio et al 2003), in which a DNA template (2µL) was amplified using 0.5mM of each primer, 1.0U Taq DNA polymerase, 0.25mM deoxynucleoside triphosphate, 1x PCR buffer and 2.5mM MgCl 2 in a 25µL reaction. Amplification was performed in a thermal-cycler (FTGene5D -Techgene, UK) with an initial denaturation step at 94°C for 10 min, followed by a 40-cycle program with denaturation (94°C; 30 s), annealing (50°C; 30 s), primer extension (72°C; 30 s), and a final extension at 72°C for 10 min.…”

Molecular detection of feline arthropod-borne pathogens in cats in Cuiabá, state of Mato Grosso, central-western region of Brazil

“…Partial sequences of the 16S rRNA gene of M. haemofelis, 'Candidatus Mycoplasma haemominutum' and 'Candidatus Mycoplasma turicensis' were amplified by means of PCR in final-volume reaction mixtures of 25 µL containing 5 µL of template DNA, 10X (2.5 µL) PCR buffer, 1.0 mM (1 µL) MgCl 2 , 0.2 mM (2 µL) deoxynucleotide triphosphate (dNTP) mixture, 1.5 U (0.25 µL) Taq DNA polymerase (Invitrogen, Carlsbad, California, USA) and 0.2 mM (1 µL) of primers that had previously been described (CRIADO-FORNELIO et al, 2003;SANTOS et al, 2009).…”