Presence of Newcastle disease viruses of sub-genotypes Vc and VIn in backyard chickens and in apparently healthy wild birds from Mexico in 2017

Ferreira, Helena Lage; Taylor, Tonya L.; Absalón, Ángel E.; Dimitrov, Kiril M.; Cortés-Espinosa, Diana V.; Butt, Salman Latif; Marín-Cruz, J. L.; Goraichuk, Iryna V.; Volkening, Jeremy D.; Suarez, David L.; Afonso, Claudio L.

doi:10.1007/s11262-019-01663-1

Cited by 15 publications

(8 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The role of backyard poultry in the epidemiology of NDV is a big concern in countries where backyard poultry rearing is common. Virulent strains of NDV have been reported from backyard poultry [ 26 – 29 ]. Thus, NDV positive apparently healthy backyard flocks have a great potential to transmit velogenic NDVs to commercial poultry.…”

Section: Discussionmentioning

confidence: 99%

Prevalence of Newcastle disease and associated risk factors in domestic chickens in the Indian state of Odisha

et al. 2022

View full text Add to dashboard Cite

Newcastle disease (ND), caused by Newcastle disease virus (NDV), is a contagious disease that affects a variety of domestic and wild avian species. Though ND is vaccine-preventable, it is a persistent threat to poultry industry across the globe. The disease represents a leading cause of morbidity and mortality in chickens. To better understand the epidemiology of NDV among commercial and backyard chickens of Odisha, where chicken farming is being prioritized to assist with poverty alleviation, a cross-sectional study was conducted in two distinct seasons during 2018. Choanal swabs (n = 1361) from live birds (commercial layers, broilers, and backyard chicken) and tracheal tissues from dead birds (n = 10) were collected and tested by real-time reverse transcription polymerase chain reaction (RT-PCR) for the presence of matrix (M) and fusion (F) genes of NDV. Risk factors at the flock and individual bird levels (health status, ND vaccination status, geographical zone, management system, and housing) were assessed using multivariable logistic regression analyses. Of the 1371 samples tested, 160 were positive for M gene amplification indicating an overall apparent prevalence of 11.7% (95% CI 10.1–13.5%). Circulation of virulent NDV strains was also evident with apparent prevalence of 8.1% (13/160; 95% CI: 4.8–13.4%). In addition, commercial birds had significantly higher odds (75%) of being infected with NDV as compared to backyard poultry (p = 0.01). This study helps fill a knowledge gap in the prevalence and distribution of NDV in apparently healthy birds in eastern India, and provides a framework for future longitudinal research of NDV risk and mitigation in targeted geographies—a step forward for effective control of ND in Odisha.

show abstract

Section: Discussionmentioning

confidence: 99%

Prevalence of Newcastle disease and associated risk factors in domestic chickens in the Indian state of Odisha

et al. 2022

View full text Add to dashboard Cite

show abstract

“…These remaining studies provided evidence of pathogen spillover to and from domestic chickens and wild birds using genomic comparisons and molecular epidemiology techniques. Economically significant viruses such as HPAIV and NDV were highly represented (50, 139,141,146,158,190,[235][236][237], whereas bacterial and parasitic species were less so (141,162,180). A contributing factor to this scarcity of evidence is likely that the mechanisms of transmission differ across pathogens and avian host species (238,239).…”

Section: Pathogen Transmission To Wild Birdsmentioning

confidence: 99%

“…Backyard flocks are implicated in maintaining enzootics of two critically important RNA viruses around the globe, NDV and HPAIV (237,(248)(249)(250)(251)(252)(253)(254)(255). This is largely due to essential differences in the biosecurity of backyard flocks and commercial flocks (238,256).…”

Section: Backyard Chickens and Biosecuritymentioning

confidence: 99%

A Review of Pathogen Transmission at the Backyard Chicken–Wild Bird Interface

2020

View full text Add to dashboard Cite

Habitat conversion and the expansion of domesticated, invasive species into native habitats are increasingly recognized as drivers of pathogen emergence at the agricultural-wildlife interface. Poultry agriculture is one of the largest subsets of this interface, and pathogen spillover events between backyard chickens and wild birds are becoming more commonly reported. Native wild bird species are under numerous anthropogenic pressures, but the risks of pathogen spillover from domestic chickens have been historically underappreciated as a threat to wild birds. Now that the backyard chicken industry is one of the fastest growing industries in the world, it is imperative that the principles of biosecurity, specifically bioexclusion and biocontainment, are legislated and implemented. We reviewed the literature on spillover events of pathogens historically associated with poultry into wild birds. We also reviewed the reasons for biosecurity failures in backyard flocks that lead to those spillover events and provide recommendations for current and future backyard flock owners.

show abstract

“…34 The MinION nanopore sequencer (Oxford Nanopore Technologies [ONT], Oxford, UK), a new DNA sequencing technology that allows for rapid, in-house, real-time detection and differentiation of IBV lineages, may be cost-effective and useful in the field. 19 Amplicon-based sequencing (AmpSeq) has also been used to amplify specific regions of Newcastle disease, 3,13 infectious laryngotracheitis, 39 Zika, 34 Ebola, 35 and influenza A viruses, 46 by simple RT-PCR and then sequencing on the MinION device. Real-time data analysis, the lack of significant start-up cost investment or maintenance expenses, simultaneous and sequential multiplexing unique to MinION, and the ability to sequence long DNA molecules so that primers are in conserved regions while the product contains the variable region are the features that make the use of this technology highly feasible in disease diagnosis.…”

Section: Introductionmentioning

confidence: 99%

Real-time, MinION-based, amplicon sequencing for lineage typing of infectious bronchitis virus from upper respiratory samples

et al. 2020

View full text Add to dashboard Cite

Infectious bronchitis (IB) causes significant economic losses in the global poultry industry. Control of IB is hindered by the genetic diversity of the causative agent, infectious bronchitis virus (IBV), which has led to the emergence of several serotypes that lack complete serologic cross-protection. Although serotyping requires immunologic characterization, genotyping is an efficient means to identify IBVs detected in samples. Sanger sequencing of the S1 subunit of the spike gene is currently used to genotype IBV; however, the universal S1 PCR was created to work from cultured IBV, and it is inefficient at detecting multiple viruses in a single sample. We describe herein a MinION-based, amplicon-based sequencing (AmpSeq) method that genetically categorized IBV from clinical samples, including samples with multiple IBVs. Total RNA was extracted from 15 tracheal scrapings and choanal cleft swab samples, randomly reverse transcribed, and PCR amplified using modified S1-targeted primers. Amplicons were barcoded to allow for pooling of samples, processed per manufacturer’s instructions into a 1D MinION sequencing library, and then sequenced on the MinION. The AmpSeq method detected IBV in 13 of 14 IBV-positive samples. AmpSeq accurately detected and genotyped both IBV lineages in 3 of 5 samples containing 2 IBV lineages. Additionally, 1 sample contained 3 IBV lineages, and AmpSeq accurately detected 2 of the 3 lineages. Strain identification, including detection of different IBVs from the same lineage, was also possible with this AmpSeq method. Our results demonstrate the feasibility of using MinION-based AmpSeq for rapid and accurate identification and lineage typing of IBV from oral swab samples.

show abstract

Presence of Newcastle disease viruses of sub-genotypes Vc and VIn in backyard chickens and in apparently healthy wild birds from Mexico in 2017

Cited by 15 publications

References 46 publications

Prevalence of Newcastle disease and associated risk factors in domestic chickens in the Indian state of Odisha

Prevalence of Newcastle disease and associated risk factors in domestic chickens in the Indian state of Odisha

A Review of Pathogen Transmission at the Backyard Chicken–Wild Bird Interface

Real-time, MinION-based, amplicon sequencing for lineage typing of infectious bronchitis virus from upper respiratory samples

Contact Info

Product

Resources

About