Presence of Nosema ceranae associated with honeybee queen introductions

Muñoz, Irene; Cepero, Almudena; Pinto, M. Alice; Martín-Hernández, Raquel; Higes, Mariano; Rúa, Pilar De la

doi:10.1016/j.meegid.2014.02.008

Cited by 23 publications

(18 citation statements)

References 81 publications

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“…However, there is an evident risk of disease spreading as a result of the intense colony movement between most regions in the Iberian Peninsula. In this sense, the increased prevalence of pathogens in Spain during the study period is not surprising, consistent with data found in other studies (Higes et al 2010a, b;Botías et al 2012;Martín-Hernández et al 2012;Muñoz et al 2014) and in other regions (Fries 2010;Traver and Fell 2011;Martin et al 2013;Bekele et al 2015). It is noteworthy that around 74 % (2006) or 86 % (2010) of the colonies presented at least one of the searched pathogenic agents (V. destructor, N. apis, and N. ceranae) showing N. apis a lower prevalence even though the samples were obtained in Spring, the peak season for N. apis infestation (Fries 2010).…”

Section: Discussionsupporting

confidence: 92%

“…The wide dispersion of N. ceranae, both in Spain and worldwide (Fries 2010;Higes et al 2010a, b;Muñoz et al 2014), is indicative of the successful colonization of this new pathogen and the limited adaptation of its new host, A. mellifera. A potential effect of this rapid expansion of N. ceranae is a reduction in genetic diversity, since invasive species may affect certain genotypes more than others, and the high risk of collapse in infected colonies (including asymptomatic colonies during the incubation period: Martín-Hernández et al 2007;Higes et al 2008).…”

Section: Introductionmentioning

confidence: 99%

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Stable genetic diversity despite parasite and pathogen spread in honey bee colonies

Jara

Muñoz

Cepero

et al. 2015

Sci Nat

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In the last decades, the rapid spread of diseases, such as varroosis and nosemosis, associated with massive honey bee colonies mortality around the world has significantly decreased the number and size of honey bee populations and possibly their genetic diversity. Here, we compare the genetic diversity of Iberian honey bee colonies in two samplings performed in 2006 and 2010 in relation to the presence of the pathogenic agents Nosema apis, Nosema ceranae, and Varroa destructor in order to determine whether parasite and pathogen spread in honey bee colonies reflects changes in genetic diversity. We found that the genetic diversity remained similar, while the incidence of N. ceranae increased and the incidence of N. apis and V. destructor decreased slightly. These results indicate that the genetic diversity was not affected by the presence of these pathogenic agents in the analyzed period. However, the two groups of colonies with and without Nosema/Varroa detected showed significant genetic differentiation (G test). A detailed analysis of the allelic segregation of microsatellite loci in Nosema/Varroa-negative colonies and parasitized ones revealed two outlier loci related to genes involved in immune response.

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Section: Discussionsupporting

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Stable genetic diversity despite parasite and pathogen spread in honey bee colonies

Jara

Muñoz

Cepero

et al. 2015

Sci Nat

Self Cite

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“…Second, our study has shown that our isolates diverged into two main clades, which include geographically unrelated samples. Although the relatively low sampling of our study does not allow broad conclusions about the global N. ceranae populations, the phylogenies we recovered suggest that our isolates have been introduced into their current locations; possibly through commercial exchange of infected honeybee colonies, hive structures or royal jelly, or by means of corbicular pollen – all cases where viable spores of N. ceranae have been recorded (Cox‐Foster et al ., ; Higes et al ., ; Klee et al ., ; Zee, ; Mutinelli, ; Munoz et al ., ).…”

Section: Discussionmentioning

confidence: 97%

Genome analyses suggest the presence of polyploidy and recent human‐driven expansions in eight global populations of the honeybee pathogen Nosema ceranae

Pelin

Selman

Aris‐Brosou

et al. 2015

Environmental Microbiology

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Nosema ceranae is a microsporidian pathogen whose infections have been associated with recent global declines in the populations of western honeybees (Apis mellifera). Despite the outstanding economic and ecological threat that N. ceranae may represent for honeybees worldwide, many aspects of its biology, including its mode of reproduction, propagation and ploidy, are either very unclear or unknown. In the present study, we set to gain knowledge in these biological aspects by re-sequencing the genome of eight isolates (i.e. a population of spores isolated from one single beehive) of this species harvested from eight geographically distant beehives, and by investigating their level of polymorphism. Consistent with previous analyses performed using single gene sequences, our analyses uncovered the presence of very high genetic diversity within each isolate, but also very little hive-specific polymorphism. Surprisingly, the nature, location and distribution of this genetic variation suggest that beehives around the globe are infected by a population of N. ceranae cells that may be polyploid (4n or more), and possibly clonal. Lastly, phylogenetic analyses based on genome-wide single-nucleotide polymorphism data extracted from these parasites and mitochondrial sequences from their hosts all failed to support the current geographical structure of our isolates.

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“…Wild honeybee populations are affected by the expansion of managed allochtone populations in Europe and associated spillover of nonlocal pathogens (De la Rúa et al., ; Muñoz et al., ; Moritz, Kraus, Kryger, & Crewe, ). Current beekeeping activities include the breeding of nonlocal subspecies and led to an introgressive hybridization, which can reduce both the colony health and survival of the local subspecies populations (De la Rúa et al., ; Meixner et al., ).…”

Section: Introductionmentioning

confidence: 99%

Contribution of European forests to safeguard wild honeybee populations

Réquier

Paillet

Laroche³

et al. 2019

CONSERVATION LETTERS

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Recent studies reveal the use of tree cavities by wild honeybee colonies in European forests. This highlights the conservation potential of forests for a highly threatened component of the native entomofauna in Europe, but currently no estimate of potential wild honeybee population sizes exists. Here, we analyzed the tree cavity densities of 106 forest areas across Europe and inferred an expected population size of wild honeybees. Both forest and management types affected the density of tree cavities. Accordingly, we estimated that more than 80,000 wild honeybee colonies could be sustained in European forests. As expected, potential conservation hotspots were identified in unmanaged forests, and, surprisingly, also in other large forest areas across Europe. Our results contribute to the EU policy strategy to halt pollinator declines and reveal the potential of forest areas for the conservation of so far neglected wild honeybee populations in Europe. K E Y W O R D SApis mellifera, conservation, forest management, honeybees, native populations, protected forests, tree cav- ities, unmanaged broadleaved forestsThis is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Presence of Nosema ceranae associated with honeybee queen introductions

Cited by 23 publications

References 81 publications

Stable genetic diversity despite parasite and pathogen spread in honey bee colonies

Stable genetic diversity despite parasite and pathogen spread in honey bee colonies

Genome analyses suggest the presence of polyploidy and recent human‐driven expansions in eight global populations of the honeybee pathogen Nosema ceranae

Contribution of European forests to safeguard wild honeybee populations

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