Presence of pancreatic-type phospholipase A2 mRNA in rat gastric mucosa and lung

Sakata, Tsuneaki; Nakamura, Etsuo; Tsuruta, Yuji; Tamaki, Mikio; Teraoka, Hiroshi; Tojo, Hiromasa; Ono, Takashi; Okamoto, Mitsuhiro

doi:10.1016/0167-4781(89)90141-3

Cited by 101 publications

(50 citation statements)

References 12 publications

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“…Mammalian 14-kDa phospholipases AZ have been classified into two groups based on their primary structure [2]. Group I enzymes exist mainly in digestive organs such as the pancreas [3,4], while group II enzymes are widely distributed in non-pancreatic tissues such as the spleen [5,6], liver [7], intestine [8] or platelets [9-l 11. The group II enzymes have also been detected in exudated fluid at inflamed sites, such as glycogen-induced ascitic fluid in rabbits [12], caseininduced ascitic fluid in rats [13], or human synovial fluid in patients with rheumatoid arthritis [14-161.…”

Section: Introductionmentioning

confidence: 99%

Exacerbation of rat adjuvant arthritis by intradermal injection of purified mammalian 14‐kDa group II phospholipase A₂

Murakami¹,

Kudo²,

Nakamura³

et al. 1990

FEBS Letters

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Appreciable phospholipase 4 activity was detected in a hind paw homogenate from rats with adjuvant arthritis or carrageenan-induced edema. The activity was neutralized by treatment with antibody raised against rat platelet secretory phospholipase 4, indicating that ICkDa group II phospholipase 4 was induced in the hind paw during the process of inflammation. Injection of purified rat platelet phospholipase A2 into the hind paw of rats with adjuvant-induced arthritis resulted in exacerbation of edema in a dose-dependent manner, whereas no effect was observed on either normal rats or animals with carrageenan-induced edema under the same experimental conditions. These results suggest the importance of mammalian group II phospholipase 4 in the pathogenesis of some types of inflammation. Phospholipase 4; Adjuvant arthritis; Carrageenan-induced edema

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Section: Introductionmentioning

confidence: 99%

Exacerbation of rat adjuvant arthritis by intradermal injection of purified mammalian 14‐kDa group II phospholipase A₂

Murakami¹,

Kudo²,

Nakamura³

et al. 1990

FEBS Letters

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“…However, we could not detect pancreatic-type PLA2, because of its low content, in pancreas or lung homogenates on immunoblot analysis. The presence of pancreatic-PLA, mRNA has also been reported in human [23] and rat [7] lung. Recently, a PLA, cross-reactive with anti-(pancreatic PLA,) immunoglobulin was purified from the soluble fraction of guinea pig lung homogenates [24].…”

Section: Discussionmentioning

confidence: 86%

“…Cloning and seguencing of PLA, cDNA from guinea pig gastric mucosa As the primary structures of pancreatic PLA, of various animal species are, in general, highly similar and because the NH,-terminal amino-acid sequence of the purified gastric PLA, exhibited commom characteristics with pancreatic PLA, [17,181, the stomach and pancreas of guinea pig were screened by RNA blot analysis using rat pancreatic-PLA, cDNA [7] as a probe. The probe hybridized well with the gastric mucosa RNA, which exhibited a mobility corresponding to 540 bp, but not with the pancreas RNA under all the conditions examined.…”

Section: Resultsmentioning

confidence: 99%

“…Total RNA and poly(A)-rich RNA from various guinea pig tissues were separated on a 1 % formaldehyde/agarose gel and transferred to a nylon membrane. The PLA, mRNA was detected as described [7] with a guinea pig gastric-PLA,-cDNA fragment as a probe. The probe cDNA was obtained by digestion of the corresponding plasmid with EcoRI and purified by electrophoresis in low-melting-point agarose.…”

Section: Methodsmentioning

confidence: 99%

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Cloning and expression of phospholipase A₂ from guinea pig gastric mucosa, its induction by carbachol and secretion in vivo

Zhao

Tojo

Nonaka

et al. 1993

European Journal of Biochemistry

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A cDNA encoding phospholipase A2 (PLA2) was cloned from guinea pig gastric mucosa using a rat pancreatic‐PLA2‐cDNA fragment as a probe. The cDNA contains an open reading frame sufficient to encode the entire amino‐acid sequence of a PLA2‐precursor protein consisting of 146 amino acids, including a putative 16‐residue signal peptide and a 6‐residue activation peptide at the NH2‐terminus. Its nucleotide sequence exhibits 70% similarity to that of rat pancreatic PLA2 cDNA. The deduced amino‐acid sequence has all the typical pancreatic PLA2 characteristics, with the exception of the substitution of Phe for Tyr at position 28 in the calcium‐binding loop of the mature enzyme. When an expression vector containing the PLA2 cDNA was transfected into COS‐7 cells, a major portion of the proenzyme was secreted into the culture medium. Northern‐blot analysis showed the mRNA was present in guinea pig lung and pancreas at much lower levels than in the stomach. The effect of carbachol, a muscarinic acetylcholine agonist, on the secretion of gastric PLA2 and on its mRNA level in the gastric mucosa were examined. PLA2 secretion into the gastric juice was maximal 30 min after the subcutaneous administration of carbachol (0.4 mg/kg). It also increased the PLA2‐mRNA level in the tissue, the maximal mRNA level being delayed about 15 min compared with that in PLA2 secretion. These results suggest that vagal stimuli may contribute to PLA2 secretion and its compensatory synthesis, and that the secreted PLA2 may participate in the digestion of dietary and biliary phospholipids in the small intestine of guinea pig.

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“…It is also involved in membrane signal transduction, cellular function, and inflammatory responses (7). Lipid membrane hydrolysis by PLA 2 results in membrane perturbation, cell degranulation, and stripping of receptors in the cell surface (46,56 enzymatic effect on arachidonic acid provides a number of inflammatory mediators via cyclooxygenase or lipoxygenase action (22). The complement system is activated by the cobra venom factor (CVF) infusion (34) through an alternative pathway.…”

Section: Effects Of Nhav On Rat Paw Edemamentioning

confidence: 99%

Pharmacological characterization of rat paw edema induced by Naja haje arabica venom

Alasmari¹

2005

J. Venom. Anim. Toxins incl. Trop. Dis

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ABSTRACT:This investigation was performed in order to assess the inflammatory response induced by Naja haje arabica venom (NhaV) in rat hind paw. The Commercially available antivenom was ineffective when administered intravenously, whereas its local administration with NhaV attenuated the edema formation (P<0.001). In conclusion, NhaV-induced edema in rat paw involves PLA 2 and adenosine mechanisms. Additionally, the use of polyspecific antivenom, intravenously, was ineffective in preventing NhaV-induced edema.KEY WORDS: rat paw edema, Naja haje arabica venom, inflammatory mediators, inhibitor, antivenom.

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Presence of pancreatic-type phospholipase A2 mRNA in rat gastric mucosa and lung

Cited by 101 publications

References 12 publications

Exacerbation of rat adjuvant arthritis by intradermal injection of purified mammalian 14‐kDa group II phospholipase A₂

Exacerbation of rat adjuvant arthritis by intradermal injection of purified mammalian 14‐kDa group II phospholipase A₂

Cloning and expression of phospholipase A₂ from guinea pig gastric mucosa, its induction by carbachol and secretion in vivo

Pharmacological characterization of rat paw edema induced by Naja haje arabica venom

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Presence of pancreatic-type phospholipase A2 mRNA in rat gastric mucosa and lung

Cited by 101 publications

References 12 publications

Exacerbation of rat adjuvant arthritis by intradermal injection of purified mammalian 14‐kDa group II phospholipase A2

Exacerbation of rat adjuvant arthritis by intradermal injection of purified mammalian 14‐kDa group II phospholipase A2

Cloning and expression of phospholipase A2 from guinea pig gastric mucosa, its induction by carbachol and secretion in vivo

Pharmacological characterization of rat paw edema induced by Naja haje arabica venom

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Exacerbation of rat adjuvant arthritis by intradermal injection of purified mammalian 14‐kDa group II phospholipase A₂

Exacerbation of rat adjuvant arthritis by intradermal injection of purified mammalian 14‐kDa group II phospholipase A₂

Cloning and expression of phospholipase A₂ from guinea pig gastric mucosa, its induction by carbachol and secretion in vivo