1972
DOI: 10.1038/newbio238109a0
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Presence of Poly (A) in the Polyribosome-associated RNA of Sindbis-infected BHK Cells

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Cited by 29 publications
(16 citation statements)
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“…A lower proportion of the 42 S RNA from sucrose gradients binds to oligo(dT)-cellulose, and although one might suggest that this was due to a greater quantity of contaminating material, particularly DNA, in the lower part of the gradients, there is also a loss of 42 S RNA relative to the other binding species when the electrophoretic profiles of total infected cell RNA are compared before and after oligo(dT)-cellulose binding (see Fig. 5)-This behaviour might suggest some heterogeneity in the poly A content of 42S RNA similar to that reported by Eaton & Faulkner (1972) in Sindbis virus RNA. However, in one of the tests used by these authors, namely the nitrocellulose filter binding assay, we find that the SFV 42 S RNA binds almost quantitatively.…”
Section: Discussionmentioning
confidence: 50%
See 1 more Smart Citation
“…A lower proportion of the 42 S RNA from sucrose gradients binds to oligo(dT)-cellulose, and although one might suggest that this was due to a greater quantity of contaminating material, particularly DNA, in the lower part of the gradients, there is also a loss of 42 S RNA relative to the other binding species when the electrophoretic profiles of total infected cell RNA are compared before and after oligo(dT)-cellulose binding (see Fig. 5)-This behaviour might suggest some heterogeneity in the poly A content of 42S RNA similar to that reported by Eaton & Faulkner (1972) in Sindbis virus RNA. However, in one of the tests used by these authors, namely the nitrocellulose filter binding assay, we find that the SFV 42 S RNA binds almost quantitatively.…”
Section: Discussionmentioning
confidence: 50%
“…These RNAs include cellular mRNA and its heterogeneous nuclear precursors (Kates, 197o;Darnell, Wall & Tushinski, I97I ;Edmonds, Vaughan & Nakazoto, 1971 ; Lee, Mendecki & Brawerman, I971), virus particle RNA (Armstrong et al 1972;Eaton & Faulkner, 1972;Yogo & Wimmer, 1972), and virus-specified RNA (Kates, 197o;Philipson et al I97I;Eaton, Donaghue & Faulkner, 1972;Pridgen & Kingsbury, 1972;Weinberg, Ben-Ishai & Newbold, 1972;Galet & Prevec, 1973;Soria & Huang, 1973). In this paper we use several different procedures to examine the virus-specific RNA from cells infected with Semliki Forest virus, an alphavirus, for the presence of poly A tracts.…”
Section: Introductionmentioning
confidence: 99%
“…1 c and 1 d). Thus it is probable that the fragmentation of the viral RNA obtained by using Method A, as well as those reported by other laboratories, is due to nueleases acting during the extraction procedures (4,9,15). As can be seen in Figures 2a and 2 c, most of the single-stranded RIgA present within the infected cells belongs to the intracellular virions.…”
Section: Discussionmentioning
confidence: 93%
“…Perhaps due to the high levels of RNase in arbovirus host ceils, only recently have data been obtained on the nature of arbovirus mRNA in infected cells. Virus-specific polysomes from Sindbis-infected BHK cells contain a mixture of 16-28S RNA molecules that have covalently bound poly(A) and may represent specific parts of the viral genome (202). The predominant species of viral RNA in polysomes of Sindbis virus-infected BHK cells (203) and SFV-infected chick cells (204) is 26S RNA, and from Sindbis-infected HeLa cells (205) it is 28S RNA.…”
Section: Class 1 Virionsmentioning
confidence: 99%