Presence of virulence genes, adhesion and invasion of<i>Arcobacter butzleri</i>

Karadas, Gül; Sharbati, Soroush; Hänel, Ingrid; Messelhäußer, Ute; Glocker, Erik-Oliver; Alter, Thomas; Gölz, Greta

doi:10.1111/jam.12245

Cited by 77 publications

(137 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The putative virulence genes showed an order of prevalence for A. butzleri (n ϭ 12), A. cryaerophilus (n ϭ 5), and A. skirrowii (n ϭ 2), i.e., 85.0% ciaB, 38.3% cj1349, 25.0% cadF, 16.7% irgA, and 3.3% hecA, similar to that previously reported (12,13). A. butzleri showed the highest prevalence of virulence genes (100% ciaB, 91.7% cj1349, 91.7% cadF, 16.7% irgA, and 8.3% hecA [n ϭ 12]).…”

Section: Discussionsupporting

confidence: 70%

“…Houf and Stephan (25) determined the ability of only 7 A. cryaerophilus strains (isolated from feces of healthy human carriers) to attach to Caco-2 cells, of which only 2 adhered. The higher adhesion and invasion capacity (5/5) observed in our study could be due to the different origins of strains, as previously proposed (9,13). A recent study compared the adhesion and invasion capacities of 3 isolates of A. butzleri from chicken meat and 3 of human origin for Caco-2 and HT-29 cells.…”

Section: Discussionsupporting

confidence: 59%

“…A recent study compared the adhesion and invasion capacities of 3 isolates of A. butzleri from chicken meat and 3 of human origin for Caco-2 and HT-29 cells. All the isolates showed adhesion and invasion of Caco-2 cells, while only 4 showed adhesion to HT-29 cells and 3 invaded the cell line (13). Two isolates from chicken and one from human showed the highest adhesion to and invasion of Caco-2 and HT-29 cells, while the other two human isolates were less adhesive to and less invasive of Caco-2 cells.…”

Section: Discussionmentioning

confidence: 94%

“…All strains of A. cryaerophilus, A. butzleri, and A. skirrowii adhered to the human intestinal Caco-2 cells, and most invaded the cell line: 5/5, 11/12, and 1/2, respectively. Previous studies with these 3 species showed that overall 55/99 adhered and 9/44 invaded Caco-2, CHO, HeLa, HEp-2, INT407, IPI-2I, or Vero cell lines (reference 3 and references therein), but only 3 studies were performed on Caco-2 cells (13,21,25 T ) and strain NC81 showed adhesion but no invasion capacity. Although this was in agreement with previous results (21), the remaining three A. cibarius strains showed an invasion capacity.…”

Section: Discussionmentioning

confidence: 99%

“…In 2012, Douidah et al (12) developed primers for these virulence genes and demonstrated their presence in strains of A. butzleri, A. cryaerophilus, and A. skirrowii. More recently, Karadas et al (13) also determined the presence of these genes by PCR in 52 strains of A. butzleri and the capacity for adhesion to and invasion of HT-29 and Caco-2 cells in six strains. In that study, no correlation between virulence gene patterns and adhesive or invasive capabilities was observed.…”

mentioning

confidence: 99%

See 4 more Smart Citations

Adherence to and Invasion of Human Intestinal Cells by Arcobacter Species and Their Virulence Genotypes

Levicán

Alkeskas

Günter

et al. 2013

Appl Environ Microbiol

View full text Add to dashboard Cite

bThe genus Arcobacter is composed of 17 species which have been isolated from various sources. Of particular interest are A. butzleri, A. cryaerophilus, and A. skirrowii, as these have been associated with human cases of diarrhea, the probable transmission routes being through the ingestion of contaminated drinking water and food. To date, only limited studies of virulence traits in this genus have been undertaken. The present study used 60 Arcobacter strains isolated from different sources, representing 16 of the 17 species of the genus, to investigate their ability to adhere to and invade the human intestinal cell line Caco-2. In addition, the presence of five putative virulence genes (ciaB, cadF, cj1349, hecA, and irgA) was screened for in these strains by PCR. All Arcobacter species except A. bivalviorum and Arcobacter sp. strain W63 adhered to Caco-2 cells, and most species (10/16) were invasive. The most invasive species were A. skirrowii, A. cryaerophilus, A. butzleri, and A. defluvii. All invasive strains were positive for ciaB (encoding a putative invasion protein). Other putative virulence genes were present in other species, i.e., A. butzleri (cadF, cj1349, irgA, and hecA), A. trophiarum (cj1349), A. ellisii (cj1349), and A. defluvii (irgA). No virulence genes were detected in strains which showed little or no invasion of Caco-2 cells. These results indicate that many Arcobacter species are potential pathogens of humans and animals.

show abstract

Section: Discussionsupporting

confidence: 70%

Section: Discussionsupporting

confidence: 59%

Section: Discussionmentioning

confidence: 94%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

See 3 more Smart Citations

Adherence to and Invasion of Human Intestinal Cells by Arcobacter Species and Their Virulence Genotypes

Levicán

Alkeskas

Günter

et al. 2013

Appl Environ Microbiol

View full text Add to dashboard Cite

show abstract

The transcriptional response of Arcobacter butzleri to cold shock

Zhang

Alter

et al. 2020

FEBS Open Bio

View full text Add to dashboard Cite

Arcobacter (A.) butzleri is an emerging zoonotic pathogen associated with gastrointestinal diseases, such as abdominal cramps and diarrhea, and is widely detected in animals, showing a high prevalence in poultry and seafood. The survival and adaptation of A. butzleri to cold temperatures remains poorly studied, although it might be of interest for food safety considerations. To address this, growth patterns of eight A. butzleri isolates were determined at 8°C for 28 days. A. butzleri isolates showed strain-dependent behavior: six isolates were unculturable after day 18, one exhibited declining but detectable cell counts until day 28 and one grew to the stationary phase level. Out of 13 A. butzleri cold shock-related genes homologous to Escherichia coli, 10 were up-regulated in response to a temperature downshift to 8°C, as demonstrated by reverse transcription-quantitative PCR. Additionally, we compared these data with the cold-shock response in E. coli. Overall, we provide a deeper insight into the environmental adaptation capacities of A. butzleri, which we find shares similarities with the E. coli cold-shock response. Arcobacter spp. is an emerging zoonotic pathogen associated with gastrointestinal diseases such as abdominal cramps and diarrhea [1-3]. Among the 29 species within the genus of Arcobacter described so far [4], A. butzleri is the most widely detected species in animals, environment and foods, with high prevalences in poultry meat and seafood samples [5-10]. Although the good environmental adaptability of A. butzleri is well known, the survival and adaptation of A. butzleri to cold remains poorly studied so far, although it might be of interest for food safety considerations. A. butzleri has an optimal growth temperature of 30°C under both aerobic and microaerobic conditions but is likely to be exposed to a wide temperature range during its transmission cycle. The minimum growth temperature of A. butzleri was initially reported to be 15°C [11]. Later, some studies reported lower limits as 10°C; for example, in Ellinghausen McCullough Johnson Harris medium [12] and in chicken meat juice, as well as in Brain Heart Infusion (BHI) broth [13]. Van Driessche and Houf [14] investigated the survival capability of A. butzleri in water with/without organic material at minimum 4°C and reported that all tested A. butzleri strains survived for at least 203 days when organic material is present in the water, indicating the best cold adaptation among all human-related Arcobacter species. With the complete genome of the A. butzleri RM4018 sequenced in 2007 [15], the understanding of adaptation to environmental stress could be improved. To date, no data describing the cold-shock response of Arcobacter spp. have been reported. Because previous phenotypic studies suggest that A. butzleri could adapt to cold temperatures, the presence of cold-related genes is presumed. The function of the cold-related proteins is best studied in Escherichia coli. Jones et al. [16] first reported that, after a cold shock from 37°C to 1...

show abstract