Presencia de residuos de medicamentos veterinarios, promotores de crecimiento y potenciadores de rendimiento en animales destinados a la producción de alimentos en la Unión Europea

Serratosa, J.; Blass, Asher; Rigau, B.; Mongrell, B; Rigau, Teresa; Tortadès, M; Tolosa, Erasmo Magalhães Castro de; Aguilar, Cristóbal N.; Ribò, O.; Balagué, J

doi:10.20506/rst.25.2.1687

Cited by 55 publications

(14 citation statements)

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“…For animals, clenbuterol is used as a veterinary drug as a bronchodilator for horses and cattle and a uterine contraction suppressant for cattle [1,7]. The action of the β 2 agonist, which increases the ratio of muscle to fat, enhances feed conversion efficiency, and overseas, clenbuterol is sometimes illegally given to livestock for growth promotion purposes.…”

Section: Introductionmentioning

confidence: 99%

Determination of Clenbuterol in Various Edible Parts of Livestock Products by LC-MS/MS and LC-MS/MS/MS Methods

Hayashi

Hamase

2021

Chromatography

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The residual status of clenbuterol in various edible parts of livestock products was investigated using the LC-MS/MS and LC-MS/MS/MS methods. The target food products were the edible tissues of pig and cattle, and clenbuterol was extracted using acetonitrile containing anhydrous sodium sulfate and sodium chloride. Following the dispersive solid-phase extraction using C18 particles and purification with an anion-exchange solid-phase extraction cartridge, the resultant test sample solutions were subjected to the LC analysis. A C18 column was used as the analytical column, and the peak of clenbuterol was eluted at 4.8 min with the gradient elution from 5% acetonitrile to 99% acetonitrile in 10 min. As a result of the LC-MS/MS analysis, interfering peaks were detected around the retention time of clenbuterol. By using the LC-MS/MS/MS analysis, which was developed as a quantitative analysis method that reduces the influence of contaminants, most of the unknown peaks were removed, and it was confirmed that the food samples obtained in Kanagawa prefecture did not contain clenbuterol.

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Section: Introductionmentioning

confidence: 99%

Determination of Clenbuterol in Various Edible Parts of Livestock Products by LC-MS/MS and LC-MS/MS/MS Methods

Hayashi

Hamase

2021

Chromatography

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“…Clenbuterol (Fig. 1), a β-agonist, has been approved for use in animals as a bronchodilator and a tocolytic in Japan and overseas [1]. It selectively stimulates the β-receptor in smooth muscle cells, thereby inducing smooth muscle relaxation.…”

Section: Introductionmentioning

confidence: 99%

Quantitative Analysis of Clenbuterol in Pig Liver by LC-MS/MS/MS

2018

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We examined the utility of LC-MS/MS/MS in the rapid quantitative analysis of clenbuterol in pig liver. Compared with LC-MS/MS in the SRM mode, which gave a chromatogram of pig liver containing interference peaks near the target component peak, LC-MS/MS/MS generated a clear chromatogram with no interference peaks. Validation of the method yielded favorable results for both LC-MS/MS in the SRM mode and LC-MS/MS/MS, with trueness values of 95.5% and 102%, repeatability of 1.9% and 5.5%, and within-laboratory reproducibility of 2.0% and 5.1%, respectively. Moreover, the recovery of clenbuterol-d 9 used as surrogate was ≥70% in both measurement methods and thus, validation was achieved in terms of both selectivity and limit of quantification. The results suggest that LC-MS/MS/MS has highly selectivity for quantitative analysis as the influence of interference peaks is suppressed, and can be used when quantification is difficult with LC-MS/MS in the SRM mode due to matrix effects.

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“…[7][8][9][10] Therefore, b-agonists are prohibited as feed additives in livestock in many countries. 11 The most commonly abused b-adrenergic agonists are clenbuterol, salbutamol and ractopamine (Fig. 1).…”

Section: Introductionmentioning

confidence: 99%

Sensitive and specific detection of a new β-agonist brombuterol in tissue and feed samples by a competitive polyclonal antibody based ELISA

Chu

Yang

et al. 2016

Anal. Methods

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Presencia de residuos de medicamentos veterinarios, promotores de crecimiento y potenciadores de rendimiento en animales destinados a la producción de alimentos en la Unión Europea

Cited by 55 publications

References 0 publications

Determination of Clenbuterol in Various Edible Parts of Livestock Products by LC-MS/MS and LC-MS/MS/MS Methods

Determination of Clenbuterol in Various Edible Parts of Livestock Products by LC-MS/MS and LC-MS/MS/MS Methods

Quantitative Analysis of Clenbuterol in Pig Liver by LC-MS/MS/MS

Sensitive and specific detection of a new β-agonist brombuterol in tissue and feed samples by a competitive polyclonal antibody based ELISA

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