Preservation and Molecular Characterization of Ancient Varieties in Spanish Grapevine Germplasm Collections

Buhner-Zaharieva, Tatiana; Moussaoui, Sawsen; Lorente, I. Miguel; Andreu, Javier; Nunez, Ramón; Ortiz, Jesús; Gogorcena, Yolanda

doi:10.5344/ajev.2010.09129

Cited by 12 publications

(9 citation statements)

References 5 publications

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“…SSRs are highly polymorphic and transferable among several species of the genus Vitis [17]. Since SSRs provide unique fingerprints for cultivar identification [18], they have been used for genetic resource characterization [19,20], parentage analysis [21,22], genetic mapping [23,24], detection of quantitative trait loci (QTLs) [25], and assisted selection [26].…”

Section: Plos Onementioning

confidence: 99%

Genetic structure and molecular diversity of Brazilian grapevine germplasm: Management and use in breeding programs

Oliveira¹,

Souza

Oliveira

et al. 2020

PLoS ONE

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The management of germplasm banks is complex, especially when many accessions are involved. Microsatellite markers are an efficient tool for assessing the genetic diversity of germplasm collections, optimizing their use in breeding programs. This study genetically characterizes a large collection of 410 grapevine accessions maintained at the Agronomic Institute of Campinas (IAC) (Brazil). The accessions were genotyped with 17 highly polymorphic microsatellite markers. Genetic data were analyzed to determine the genetic structure of the germplasm, quantify its allelic diversity, suggest the composition of a core collection, and discover cases of synonymy, duplication, and misnaming. A total of 304 alleles were obtained, and 334 unique genotypes were identified. The molecular profiles of 145 accessions were confirmed according to the literature and databases, and the molecular profiles of more than 100 genotypes were reported for the first time. The analysis of the genetic structure revealed different levels of stratification. The primary division was between accessions related to Vitis vinifera and V. labrusca, followed by their separation from wild grapevine. A core collection of 120 genotypes captured 100% of all detected alleles. The accessions selected for the core collection may be used in future phenotyping efforts, in genome association studies, and for conservation purposes. Genetic divergence among accessions has practical applications in grape breeding programs, as the choice of relatively divergent parents will maximize the frequency of progeny with superior characteristics. Together, our results can enhance the management of grapevine germplasm and guide the efficient exploitation of genetic diversity to facilitate the development of new grape cultivars for fresh fruits, wine, and rootstock.

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Section: Plos Onementioning

confidence: 99%

Genetic structure and molecular diversity of Brazilian grapevine germplasm: Management and use in breeding programs

Oliveira¹,

Souza

Oliveira

et al. 2020

PLoS ONE

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“…However, this process is carried out on adult plants; a longer period is required before the identification of accessions and often it is not conclusive on the distinction of close cultivars. As many synonyms or homonyms exist for cultivars, passport data are not always sufficient to certify identities, and errors can arise (Buhner-Zaharieva et al, 2010;Laucou et al, 2011).…”

Section: Dna Isolation Rapd and Issr Amplificationmentioning

confidence: 99%

A comparative analysis of genetic diversity in Portuguese grape germplasm from ampelographic collections fit for quality wine production

Castro

Pinto-Carnide

Ortiz

et al. 2017

Span. j. agric. res.

Self Cite

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ance of millions of hectares produced by the attack of that insect. More recently, genetic diversity has suffered another drawback. New plantations with foreign material with low genetic variability have reinforced genetic erosion of native germplasm. Moreover, European Union incentives for restructuring and conversion, particularly in Portugal and Spain, have conducted to RESEARCH ARTICLE OPEN ACCESS AbstractGrapevine cultivars diversity is vast and full of synonyms and homonyms. Up to few decades ago characterization of grapevine was based on morphological characters. In the last decades, molecular markers were developed and have been used as tools to study genetic diversity in a range of different plant species. Fifty-six Portuguese accessions representative of 'Vinhos Verdes' and 'Douro' Controlled Designations of Origin (DOC) were analysed through DNA fingerprints generated by Random Amplified Polymorphic DNA (RAPD) and Inter Simple Sequence Repeat (ISSR). The study aimed to compare the effectiveness of RAPD and ISSR molecular techniques in the detection of synonyms, homonyms and misnames. RAPD and ISSR analysis enabled the detection of 36 different band patterns, reducing in about 36% the initial material. Several accessions grown under different names, between and within collections, were confirmed as the same genotype, namely Gouveio/Verdelho, Sousão Douro/Vinhão and Arinto Oeste/ Pedernã. Similarly, some homonyms/misnames were also identified, namely within Azal Tinto and Rabigato accessions. RAPD and ISSR markers revealed to be adequate molecular techniques for grapevine varieties fingerprinting with advantages over other molecular procedures, contributing for a good management of grapevine collections.

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“…According to García de los Salmones [7], the first Phylloxera outbreak in Spain was detected in Malaga (Andalusia) in 1876. From that moment on, this pathogen spread throughout the whole country and destroyed more than 1,000,000 ha, which caused serious damages to the Spanish native germplasm [8]. In order to preserve the maximum number of Vitis vinifera genetic diversity, a number of germplasm banks were created.…”

Section: Introductionmentioning

confidence: 99%

“…From then on, the prospection, collection and conservation of different grapevine cultivars as a genetic resource have been the subject of numerous studies that intend to preserve those cultivars considered as autochthonous [7][8][9][10][11]. For the identification of that genetic material, molecular characterization using Simple Sequence Repeats (SSR) markers [12], ampelographic [4] and physicochemical [13] techniques have been used.…”

Section: Introductionmentioning

confidence: 99%

Genetical, Morphological and Physicochemical Characterization of the Autochthonous Cultivar ‘Uva Rey’ (Vitis vinifera L.)

et al. 2019

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‘Uva Rey’ is considered an Andalusian (Spain) ancient autochthonous cultivar with hard white grapes used for the production of wine and raisins and also for raw consumption. Currently, this cultivar is not included in the official register of Spanish grapevine varieties and there is neither a description nor a characterization that could facilitate its insertion in this register. In order to study this genetic resource, a genetic and morphological characterization of ‘Uva Rey’ has been carried out in comparison with ‘Palomino Fino’, the main cultivar in Andalusia (Spain). Additionally, grape must physicochemical characterization and grape berry texture profile analyses were performed. Genetically, ‘Uva Rey’ was synonymous with the cultivar ‘De Rey’. ‘Uva Rey’ grape must physicochemical results showed a lower sugar concentration and a higher malic acid content compared to ‘Palomino Fino’ must, while the analysis of the grape berry texture profile proved to be more consistent and cohesive. These results can be attributed to the longer phenological cycle presented by ‘Uva Rey’. All these facts could lead to consideration of ‘Uva Rey’ as a cultivar for the production of white wines in warm climate regions.

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Preservation and Molecular Characterization of Ancient Varieties in Spanish Grapevine Germplasm Collections

Cited by 12 publications

References 5 publications

Genetic structure and molecular diversity of Brazilian grapevine germplasm: Management and use in breeding programs

Genetic structure and molecular diversity of Brazilian grapevine germplasm: Management and use in breeding programs

A comparative analysis of genetic diversity in Portuguese grape germplasm from ampelographic collections fit for quality wine production

Genetical, Morphological and Physicochemical Characterization of the Autochthonous Cultivar ‘Uva Rey’ (Vitis vinifera L.)

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