Preservation of algaenan and proteinaceous material during the oxic decay of Botryococcus braunii as revealed by pyrolysis-gas chromatography/mass spectrometry and 13 C NMR spectroscopy

Nguyen, Reno T.; Harvey, H. Rodger; Zang, Xu; Heemst, Jasper D.H van; Hetényi, Magdolna; Hatcher, Patrick G.

doi:10.1016/s0146-6380(02)00261-9

Cited by 63 publications

(49 citation statements)

References 34 publications

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“…These Nheterocyclic compounds often were explained by their formation during Cp pyrolysis, as shown for individual amino acids and peptides (Bracewell and Robertson 1984;Schulten et al 1995). Pyrolysis products were reported for lysine (pyridine) (Sorge 1995), hydroxyproline, proline (1H-pyrrole, 1-methyl-) (Nguyen et al 2003), hydroxyproline (1H-pyrrole, 2,5-dimethyl-) (Chiavari and Galletti 1992), alanine, tyrosine (pyridine) (Chiavari and Galletti 1992;Nguyen et al 2003), threonine (pyridine, 5-ethenyl-2-methyl-) (Sorge et al 1993), and phenylalanine (quinoline) (Patterson et al 1973) (pyrolysis product in parenthesis). However, such a formation of N-heterocyclic compounds as pyrolysis products does not necessarily exclude their genuine occurrence in soils (Leinweber and Schulten 1998), especially if we consider recently described abiotic pathways for the formation of heterocyclic N (Jokic et al 2004a;Huang and Hardie 2009) and the impact of vegetation fires on soils (Knicker 2009).…”

Section: Resultsmentioning

confidence: 97%

Nitrogen speciation in fine and coarse clay fractions of a Cryoboroll - new evidence from pyrolysis-mass spectrometry and nitrogen K-edge XANES

Leinweber

Jandl²,

Eckhardt³

et al. 2010

Can. J. Soil. Sci.

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Nitrogen speciation in fine and coarse clay fractions of a Cryoboroll Á new evidence from pyrolysis-mass spectrometry and nitrogen K-edge XANES. Can. J. Soil Sci. 90: 309Á318. Soil clay fractions are usually enriched in nitrogen (N), but the chemical identity of this N is largely unknown. Therefore, we investigated organic N in fine and coarse clay of a clay-rich Cryoboroll by Curie-point pyrolysis-gas chromatography/mass spectrometry (Cp Py-GC/MS), Pyrolysis-field ionization mass spectroscopy (Py-FIMS) and synchrotron-based nitrogen K-edge X-ray absorption near edge structure (N-XANES) spectroscopy. The Cp Py-GC-MS revealed 30 structurally different N-containing compounds, such as substituted pyridines, pyrroles; pyrazines, pyrazoles, imidazoles, quinolines, side-chain N-containing benzenes, and single compounds of substituted benzotriazole, purine and indole. These accounted for about 10% of peak area in the Py-GC chromatograms. The Py-FIMS and N-XANES spectra indicated interlayer-NH 4 ' and revealed pyridinic and nitrilic N compounds, but disagreed in the proportions of pyrroles. All three complementary methods confirmed to different extents previous wet-chemical data on N-fractions in these samples, and provided new evidence for about 30 to 40% non-proteinaceous N as major constituent of the so-called ''unknown organic N'' in soil.

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Section: Resultsmentioning

confidence: 97%

Nitrogen speciation in fine and coarse clay fractions of a Cryoboroll - new evidence from pyrolysis-mass spectrometry and nitrogen K-edge XANES

Leinweber

Jandl²,

Eckhardt³

et al. 2010

Can. J. Soil. Sci.

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“…In follow-up studies, Harvey and coworkers [91][92][93] provided further support for the encapsulation hypothesis. Zang et al [91] conducted dual-labeling experiments using 13 C and 15 N to follow the degradation of Botryococcus braunii, a prolific producer of biopolymeric algaenan.…”

Section: Matrix Effectsmentioning

confidence: 93%

Degradation and Preservation of Organic Matter in Marine Sediments

Wakeham

Canuel

The Handbook of Environmental Chemistry

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“…The overall loss of identified cytoplasm proteins was more rapid than the loss of chloroplast proteins, but identified translation proteins located in the chloroplast were lost just as rapidly as translation proteins located in the ribosome or cytoplasm. In previous studies it has been observed that proteins encapsulated in organic matter or cellular compartments are preferentially preserved during microbial recycling (Nguyen et al 2003, Nunn et al 2010, Moore et al 2012a). Cell lysis may have played a role in the loss of cytoplasmic translation proteins, but the rapid loss of identifiable translation proteins with additional membrane protection in cellular compartments indicates that some loss was due to internal recycling within the cell in tandem with microbial activity.…”

Section: Differential Recycling Of Algal Proteins and Preservationmentioning

confidence: 99%

Protein recycling in Bering Sea algal incubations

Moore

Harvey

Faux

et al. 2014

Mar. Ecol. Prog. Ser.

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Protein present in phytoplankton represents a large fraction of the organic nitrogen and carbon transported from its synthesis in surface waters to marine sediments. Yet relatively little is known about the longevity of identifiable protein in situ, or the potential modifications to proteins that occur during bloom termination, protein recycling and degradation. To address this knowledge gap, diatom-dominated phytoplankton was collected during the Bering Sea spring blooms of 2009 and 2010, and incubated under darkness in separate shipboard degradation ex periments spanning 11 and 53 d, respectively. In each experiment, the protein distribution was monited over time using shotgun proteomics, along with total hydrolyzable amino acids (THAAs), total protein, particulate organic carbon (POC) and nitrogen (PN), and bacterial cell abundance. Identifiable proteins, total protein and THAAs were rapidly lost during the first 5 d of enclosure in darkness in both incubations. Thereafter the loss rate was slower, and it declined further after 22 d. The initial loss of identifiable biosynthetic, glycolysis, metabolism and translation proteins after 12 h may represent shutdown of cellular activity among algal cells. Additional peptides with glycan modifications were identified in early incubation time points, suggesting that such protein modifications could be used as a marker for internal recycling processes and possibly cell death. Protein recycling was not uniform, with a subset of algal proteins including fucoxanthin chlorophyll binding proteins and RuBisCO identified after 53 d of degradation. Non-metric multidimensional scaling was used to compare the incubations with previous environmental results. The results confirmed recent observations that some fraction of algal proteins can survive water column recycling and undergo transport to marine sediments, thus contributing organic nitrogen to the benthos.

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Preservation of algaenan and proteinaceous material during the oxic decay of Botryococcus braunii as revealed by pyrolysis-gas chromatography/mass spectrometry and 13 C NMR spectroscopy

Cited by 63 publications

References 34 publications

Nitrogen speciation in fine and coarse clay fractions of a Cryoboroll - new evidence from pyrolysis-mass spectrometry and nitrogen K-edge XANES

Nitrogen speciation in fine and coarse clay fractions of a Cryoboroll - new evidence from pyrolysis-mass spectrometry and nitrogen K-edge XANES

Degradation and Preservation of Organic Matter in Marine Sediments

Protein recycling in Bering Sea algal incubations

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