Preservation of Epididymal Stallion Sperm in Liquid and Frozen States: Effects of Seminal Plasma on Sperm Function and Fertility

Miró, Jordi; Morató, Roser; Vilagran, Ingrid; Taberner, E.; Bonet, Sergi; Yeste, Marc

doi:10.1016/j.jevs.2020.102940

Cited by 3 publications

(2 citation statements)

References 34 publications

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“…Numerous harvesting methods of epididymal semen have been evaluated and optimized in the domesticated species [8,[13][14][15][16]. In horses, the methods available for epididymal semen harvesting include direct aspiration, slicing, and flotation, or retrograde flushing of the cauda epididymis; [8,17]. Although there is no consensus regarding the most suitable method, the latter appears to be the most widely used [10].…”

Section: Introductionmentioning

confidence: 99%

Donkey Epididymal Transport for Semen Cooling and Freezing

Lago-Alvarez

Podico

Segabinazzi

et al. 2020

Animals

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The objectives of this study were to assess the cooling and freezing of donkey epididymal semen harvested immediately after castration (Experiment 1, n = 4) or after the shipment (24 or 48 h) of epididymides attached to testicles (Experiment 2, n = 14) or dissected apart (Experiment 3, n = 36). In each experiment, semen was frozen immediately (Non-Centrif) in an egg yolk-based semen extender (EY) or after processing through cushion-centrifugation (Centrif) while extended in a skim milk-based extender (SC). In all three experiments, cooled, pre-freeze, and post-thaw epididymal semen was assessed for total motility (TM), progressive motility (PM), plasma membrane integrity (PMI), and high mitochondrial membrane potential (HMMP). Data were analyzed with R using mixed models and Tukey’s test as posthoc. Results showed that the cooling of epididymal semen up to 24 h after harvesting did not affect motility parameters or plasma membrane integrity; furthermore, in Experiment 3, the post-thaw evaluation of both Centrif and Non-Centrif achieved similar TM and PM. Collectively, the post-thaw results revealed low motility parameters across groups; while, the PMI and HMMP did not reflect this trend, and the values remained high, suggesting that there was a lack of epididymal sperm activation with either centrifugation or extenders. In summary, freshly harvested and cooled-shipped and cooled semen had satisfactory semen parameters. Future studies need to address donkey epididymal semen fertility in mares and jennies.

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Section: Introductionmentioning

confidence: 99%

Donkey Epididymal Transport for Semen Cooling and Freezing

Lago-Alvarez

Podico

Segabinazzi

et al. 2020

Animals

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“…Therefore, epididymal spermatozoa (EPS) collection and cryopreservation provides the cheapest and easiest alternative means of collecting spermatozoa to conserve AGRs of a valuable sire in the case of sudden death or castration [2]. Moreover, studies in different species proved that EPS resulted in good-to-excellent pregnancy rates following AI in boar (92.0%) [3], cattle (58.8%) [4], sheep (87.5%, 58.5%, and 55.0%) [5][6][7], goats (61.2%) [8], stallions (27.8% and 64.0%) [9,10], and red deer (75.0%) [11]. Therefore, it is considered the most viable, cheapest, and easiest way to conserve the genetic resources of endangered, threatened, or valuable animals that die accidentally.…”

Section: Introductionmentioning

confidence: 99%

The Influence of Three Commercial Soy Lecithin-Based Semen Extenders and Two Spermatozoa Concentrations on the Quality of Pre-Freeze and Post-Thaw Ram Epididymal Spermatozoa

Mujitaba,

Kútvölgyi,

Radnai Szentpáli

et al. 2024

Animals

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There are limited studies on the factors affecting the success of ram epididymal spermatozoa (REPS) cryopreservation. On this note, the current study assessed the influence of three commercial soy lecithin-based semen extenders, AndroMed® (AND), BioXcell® (BIO), and OviXcell® (OVI), and two concentrations (400 × 106 vs. 200 × 106 spermatozoa/mL) on the pre-freeze and post-thaw quality of REPS. The REPS were retrieved from nine adult rams’ testes and diluted with each of the three extenders to both concentrations. Straws were frozen manually. Standard motility (SMP) and kinematic parameters (KPs) were assessed via a CASA, while spermatozoa viability, morphology, and acrosomal integrity were assessed via the Kovács–Foote staining technique. The concentration did not significantly affect the pre-freeze and post-thaw SMP and KPs of REPS. BIO and OVI had significantly higher pre-freeze and post-thaw BCFs, post-thaw VAP, and the percentage of all intact heads than AND. In contrast, AND had a significantly lower percentage of REPS with tail defects than BIO and OVI. The 400 × 106 spermatozoa/mL concentration resulted in a significantly higher percentage of all intact heads than the 200 × 106 spermatozoa/mL concentration. Freezing significantly increased tail defects and decreased the percentage of REPS with distal cytoplasmic droplets. The cryopreservation of REPS at the 400 × 106 spermatozoa/mL concentration is recommended. All three extenders must be optimized to preserve the viability, membrane integrity, and better normal morphology of REPS; the reason for increased tail abnormality after the freezing/thawing process needs to be studied.

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Role of Seminal Plasma in the Equine Reproduction

Roig,

Catalán

2024

Assisted Reproductive Technologies in Animals Volume 1

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Preservation of Epididymal Stallion Sperm in Liquid and Frozen States: Effects of Seminal Plasma on Sperm Function and Fertility

Cited by 3 publications

References 34 publications

Donkey Epididymal Transport for Semen Cooling and Freezing

Donkey Epididymal Transport for Semen Cooling and Freezing

The Influence of Three Commercial Soy Lecithin-Based Semen Extenders and Two Spermatozoa Concentrations on the Quality of Pre-Freeze and Post-Thaw Ram Epididymal Spermatozoa

Role of Seminal Plasma in the Equine Reproduction

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