Preservation of (−)-Epigallocatechin-3-gallate Antioxidant Properties Loaded in Heat Treated β-Lactoglobulin Nanoparticles

Abstract

(-)-Epigallocatechin-3-gallate (EGCG) was loaded in heat treated β-lactoglobulin (β-Lg) for the preservation of antioxidant activity. The effects of pH (2.5-7.0), the heating temperature of β-Lg (30-85 °C), the molar ratio of β-Lg to EGCG (1:2-1:32), and the β-Lg concentration (1-10 mg/mL) on the properties of β-Lg-EGCG complexes were studied. All four factors significantly influenced the particle size, the ζ-potential, and the entrapment efficiency of EGCG and EGCG loading in β-Lg particles. A stable and clear solution system could be obtained at pH 6.4-7.0. The highest protection of EGCG antioxidant activity was obtained with β-Lg heated at 85 °C and the molar ratio of 1:2 (β-Lg: EGCG). β-Lg-EGCG complexes were found to have the same secondary structure as native β-Lg.

Physical effects upon whey protein aggregation for nano-coating production

“…Li designed the encapsulation of epigallocatechin-3-gallate (EGCG), a potent antioxidant obtained from green tea, in nanocoatings of β-Lg. A stable and clear nanosystem was observed and the highest protection of EGCG antioxidant activity was obtained with β-Lg heated at 85 °C and at the molar ratio of 1:2 (β-Lg:EGCG) (Li, Du, Jin, & Du, 2012).…”

Design of whey protein nanostructures for incorporation and release of nutraceutical compounds in food

“…Bengoechea et al prepared nanohydrogels from bovine lactoferrin by a simple thermal method, able to resist to subsequent pH (from 3 to 11) and salt (from 0 to 200 mM NaCl) alterations, being useful as carriers systems or functional ingredients in food products (Bengoechea et al, 2011). Li et al designed encapsulation of epigallocatechin-3-gallate (EGCG), the major catechin in green tea and a potent antioxidant, in nanohydrogels of -Lg (Li et al, 2012). A stable and clear nanosystem was observed at pH 6.4-7.0, and highest protection of EGCG antioxidant activity was obtained with -Lg heated at 85 o C and at the molar ratio of 1:2 (-Lg: EGCG).…”

Natural Compounds as Therapeutic Agents for Amyloidogenic Diseases

“…OLC (3, is the dialdehydic form of the decarboxymethyl derivative of ligstroside aglycone 3,4-hydroxyphenylethanol elenolic acid (3,4-HPEA-EA) (Servili et al 1999) ( Fig. 1.3).…”

“…A chemically-standardized water extract of KXS applied to astrocytes significantly stimulated the Table 5. 3 The top Chinese herbs for improving memory, their major constituents, anti-dementia and neuroprotective actions Chinese herbs and constituents Therapeutic and anti-dementia bioactivities P. cocos (a medicinal mushroom) triterpenes, pachymic acid, dehydropachymic acid.…”

“…Inhibits deposits in vivo (animal models) 3,4,6,7,10,11,15 (continued) (Solomon et al 1982) and in Transthyretin-Amyloidosis, respectively (Saraiva et al 1984). In Diabetes Mellitus type II (DM II) the Islet Amyloid Polypeptide (IAPP, amylin) forms amyloid deposits in the insulin producing islet-"-cells (Johnson et al 1989).…”