Preservation of fine‐needle aspiration specimens for future use in RNA‐based molecular testing

Ladd, Amy C.; O’Sullivan‐Mejia, Emerald; Lea, Tasha; Perry, Jessica; Dumur, Catherine I.; Dragoescu, Ema; Garrett, Carleton T.; Powers, Celeste N.

doi:10.1002/cncy.20130

Cited by 35 publications

(34 citation statements)

References 16 publications

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“…However, as the aim of this study was to compare different approaches in parallel for the detection of EGFR mutations from MPE samples, this limitation should not hamper our objective. Moreover, although our study suggested the promise of using RNA for EGFR analysis, the inherently labile nature of RNA, as well as the ubiquitous presence of RNase, warrants careful sample processing in RNA-based molecular testing [46].…”

Section: Lung Cancer T-h Tsai Et Almentioning

confidence: 87%

RNA is favourable for analysingEGFRmutations in malignant pleural effusion of lung cancer

Tsai

Su²,

Wu³

et al. 2011

Eur Respir J

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Malignant pleural effusion (MPE) is a useful specimen allowing for the evaluation of EGFR status in nonsmall cell lung cancer (NSCLC). However, direct sequencing of genomic DNA from MPE samples was found not to be sensitive for EGFR mutation detection.To test whether EGFR analysis from RNA is less prone to interference from nontumour cells that have no or lower EGFR expression, we compared three methods (sequencing from cell-derived RNA versus sequencing and mass-spectrometric analysis from genomic DNA), in parallel, for EGFR mutation detection from MPE samples in 150 lung adenocarcinoma patients receiving firstline tyrosine kinase inhibitors (TKIs).Among these MPE samples, EGFR mutations were much more frequently identified by sequencing using RNA than by sequencing and mass-spectrometric analysis from genomic DNA (for all mutations, 67.3 versus 44.7 and 46.7%; for L858R or exon 19 deletions, 61.3 versus 41.3 and 46.7%, respectively). The better mutation detection yield of sequencing from RNA was coupled with the superior prediction of clinical efficacy of first-line TKIs. In patients with acquired resistance, EGFR sequencing from RNA provided satisfactory detection of T790M (54.2%).These results demonstrated that EGFR sequencing using RNA as template greatly improves sensitivity for EGFR mutation detection from samples of MPE, highlighting RNA as the favourable source for analysing EGFR mutations from heterogeneous MPE specimens in NSCLC.

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Section: Lung Cancer T-h Tsai Et Almentioning

confidence: 87%

RNA is favourable for analysingEGFRmutations in malignant pleural effusion of lung cancer

Tsai

Su²,

Wu³

et al. 2011

Eur Respir J

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“…[57][58][59][60][61][62] A variety of cytology platforms have been validated for mutation testing. 63 Ninety-eight percent of lung cytology samples were suitable for epidermal growth factor receptor and KRAS mutation testing in a recent study.…”

Section: Targeted Therapies Based On Cytologymentioning

confidence: 99%

Five Top Stories in Cytopathology

Fischer,

Benedict,

Amrikachi

2013

Archives of Pathology &Amp; Laboratory Medicine

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Context.-Cytology relies heavily on morphology to make diagnoses, and morphologic criteria have not changed much in recent years. The field is being shaped predominantly by new techniques for imaging and for acquiring and processing samples, advances in molecular diagnosis and therapeutics, and regulatory issues.Objective.-To review the importance of classical morphology in the future of cytopathology, to identify areas in which cytology is expanding or contracting in its scope, and to identify factors that are shaping the field.Data Sources.-Literature review.Conclusions.-Five stories paint a picture in which classical cytomorphology will continue to have essential importance, both for diagnosis and for improving our understanding of cancer biology. New endoscopy and imaging techniques are replacing surgical biopsies with cytology samples. New molecularly targeted therapies offer a chance for cytology to play a major role, but they pose new challenges. New molecular tests have the potential to synergize with, but not replace, morphologic interpretation of thyroid fine-needle aspirations. Ultrasound-guided fine-needle aspiration performed by cytopathologists is opening a new field of ''interventional cytopathology'' with unique value. For the productive evolution of the field, it will be important for cytopathologists to play an active role in clinical trials that document the ability of cytology to achieve cost-effective health care outcomes.(Arch Pathol Lab Med. 2013;137:894-906; doi: 10.5858/ arpa.2012-0258-SA) THE CONTINUING VALUE OF CYTOMORPHOLOGY C ytopathologists have been primarily morphologists, using the smallest possible biopsy specimen for diagnosis. It is unusual for new diagnostic morphologic features to be discovered. A rare example is the description of intranuclear cytoplasmic inclusions in at least a subset of pancreatic intraductal papillary mucinous neoplasms.1 The new Bethesda system for reporting thyroid cytopathology is certainly important, but the actual morphologic criteria for diagnosing thyroid fine-needle aspirations (FNAs) have scarcely changed in the past several decades. It seems that classical cytomorphology has brought the field about as far as it can go.Though new diagnostic cytomorphologies are not likely to help the productive evolution of cytology, the theme of this first story is that classical morphology will continue to have an essential value. In order to provide new useful clinical applications, cytopathology needs to be redefined as the field that uses the smallest possible biopsy specimen for diagnosis by using any technique. The full value of classical cytomorphology can be boosted by new technologies, including improvements in cell block techniques 2-4 and better microbiopsy needles. 5 The use of ultrasound guidance for cytopathologist-performed FNA and the development of new endoscopic techniques have provided new applications for classical cytomorphology. New immunofluorescent microscopy techniques are promising to synergize with classical cytomorphologic diagnosis...

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“…It recently was demonstrated that the cellular morphology and RNA integrity necessary for studies of gene expression are maintained with cryopreserved samples. 15 The detection of phosphorylated signaling proteins in FNAs for the measurement of pathway activity using multiplexed analysis has been described and presents the opportunity for its application in drug response studies and functional assays. 16 New miniaturized devices using different platforms may add useful data to morphological assessment and could be integrated into the clinical routine to improve the speed with which results are determined.…”

Section: Other Alternatives and Novel Specimen Preservation Techniquesmentioning

confidence: 99%

Maximizing the yield of lymph node cytology

Santos¹,

Boerner²,

Geddie³

2011

Cancer Cytopathology

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The evaluation of mediastinal and hilar lymph nodes for tissue diagnosis and staging of lung cancer is now commonly performed by minimally invasive, nonsurgical procedures such as computed tomography‐guided fine‐needle aspiration and endobronchial ultrasound‐guided transbronchial needle aspiration. Ensuring that a sufficient quantity of cellular material has been acquired to enable multiple studies has become a priority issue in the era of personalized medicine, especially for patients with lung cancer, and this can be accomplished by rapid onsite evaluation (ROSE). This commentary focuses on the use of ROSE in guided procedures, especially for hilar and mediastinal lymph node aspirates, and describes an algorithm for handling these specimens. Cancer (Cancer Cytopathol) 2011;. © 2011 American Cancer Society.

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Preservation of fine‐needle aspiration specimens for future use in RNA‐based molecular testing

Cited by 35 publications

References 16 publications

RNA is favourable for analysingEGFRmutations in malignant pleural effusion of lung cancer

RNA is favourable for analysingEGFRmutations in malignant pleural effusion of lung cancer

Five Top Stories in Cytopathology

Maximizing the yield of lymph node cytology

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