Pressinoic acid: A peptide with potent corticotrophin-releasing activity

Saffran, Murray; Pearlmutter, A. Frances; Rapino, Eloise; Upton, G. Virginia

doi:10.1016/0006-291x(72)90474-3

Cited by 12 publications

(3 citation statements)

References 14 publications

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“…If this is the case it might be expected that the polypeptides bear a structural resemblance to HME-CRF, so that they are able to activate the ACTH secretion mechanism, but unable to do so with full efficacy. In this regard, the recent report of Saffran et al (31) that the ring portion of vasopressin, pressinoic acid, is a more potent CRF than lys-vasopressin itself is of interest, perhaps indicating that this compound is structurally more similar to CRF than the intact neurohypophysial hormone.…”

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confidence: 91%

Isolated Pituitary Cells: CRF-Like Activity of Neurohypophysial and Related Polypeptides

Portanova¹,

Sayers²

1973

Experimental Biology and Medicine

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The role of vasopressin in the regulation of corticotropin (ACTH) secretion is enigmatic: in spite of repeated observations that vasopressin is an effective stimulus to ACTH release (2)(3)(4)(5) there is much evidence to indicate that it is chemically distinct from the psysiologically important hypothalamic mediator of ACTH secretion, corticotropin releasing factor (CRF) [see (6, 7)]. We now present studies which, we believe, provide some insight into this problem: the CRF-like activity of vasopressin and related polypeptides is distinguished from that of hypothalamic median eminence (HME) extract by their bioassay (log dose response) characteristics and evidence is presented that these polypeptides are partial agonists of ACTH secretion.Materials and Methods. CRF bioassays were conducted by determining the rate of secretion of ACTH from isolated pituitary cells incubated in the presence of graded doses of test substance (polypeptide or HME extract). The techniques employed for the preparation and incubation of these cells have been described in detail (8,9). In brief, pituitary cells were dispersed from the anterior lobes of male Sprague-Dawley rats by a combination of trypsin and mechanical agitation; intact animals, or in mme cases, as indicated, animals adrenalectomized 2 wk previously and maintained on 0.9% saline drinking solution were used as donors of pituitary tissue. The cells were collected by centrifugation and resuspended to a concentration of 1 pituitary equiv/ml in Krebs-Ringer bicarbonate buffer containing glucose (0.2%), bovine serum albumin (0.5%), and lima bean trypsin inhibitor (0.2 %) . Ali-1 A preliminary report of this work hmas {appeared in abstraot form ( 1 ) . This work was supported by US. Public Healt'h Service Grant AM-13820.quots of cell suspensions were incubated for 40 min in the presence of vehicle (controls) or various doses of test substance (polypeptide or HME extract); the cells were removed by centrifugation and the separated medium was acidi'fied and appropriately diluted for ACTH bioassay as described by Sayers, Swallow and Giordano ( 10).Acid extracts of rat HME were prepared as previously described (9). Natural argvasopressin was extracted from bovine neurohypophyses (1 1) and had a pressor activity of 320 mU/pg ( 1 2 ) . Synthetic argvasopressin, lys-vasopressin, 8-homolys-vasopressin, l-deamino-8-homolys-vasopressin, oxytocin, and pro-arg-gly-NH2 were kindly provided by Dr. M. Bodanszky, #Case Western Reserve University, Cleveland, OH ; synthetic arg-vasotocin, [ N-~t-,triglycly-8-lys] vasopressin, [ 1-N-carbamoyl-hemicystine-2o-methyltyrosine] -oxytocin, and pro-leu-gly-NH2 were kindly provided by Dr. J. Rudinger,

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confidence: 91%

Isolated Pituitary Cells: CRF-Like Activity of Neurohypophysial and Related Polypeptides

Portanova¹,

Sayers²

1973

Experimental Biology and Medicine

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“…Pressinoic acid, the ring structure of vasopressin once thought to have CRF activity (223), showed no CRF activity in several subsequent studies (36, 109, 144). Aizawa et al (109) recently found that the CRF and pressor activities of 21 vasopressin analogs were highly correlated, but CRF and antidiuretic activities were not.…”

Section: Role Of Vasopressin and Related Peptides In Stimulating Acthmentioning

confidence: 98%

Corticotropin-Releasing Factor*

1982

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“…Physiologically, vasopressin acts as part of a complex responsible for the Control of ACTH release (Yates et al, 1971;Portanova & Sayers, 1973). As with oxytocin, it appears that a fragment of the molecule, in this case the ring-structure (pressinoic acid), may be considerably more potent than the intact molecule (Saffran et al, 1972). There is also evidence that vasopressin and related peptides may have important central effects, especially with respect to conditioned learning processes (de Wied, 1971).…”

Section: The Functions Of Oxytocin and Vasopressinmentioning

confidence: 99%

The Posterior Pituitary Gland

Chard

1975

Clinical Endocrinology

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Pressinoic acid: A peptide with potent corticotrophin-releasing activity

Cited by 12 publications

References 14 publications

Isolated Pituitary Cells: CRF-Like Activity of Neurohypophysial and Related Polypeptides

Isolated Pituitary Cells: CRF-Like Activity of Neurohypophysial and Related Polypeptides

Corticotropin-Releasing Factor*

The Posterior Pituitary Gland

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