Pressure and temperature dependence of fluorescence anisotropy of green fluorescent protein

Abstract

Effect of high hydrostatic pressure and temperature on the steady state fluorescence anisotropy of Green Fluorescent Protein (GFP).

Influence of Viscosity and Molecular Size on Temperature Sensitivity of Fluorescence Anisotropy

“…This difference in the contribution is mainly due to the large T / η rather than the temperature-dependent viscosity ∂η / ∂T. The importance of the viscosity term was also implied by Kaur et al, (18) and our study quantitatively supports this. The disagreement for the FITC-dextran conjugates may be due to the complex structure of dextran, which consists of many glucose molecules linked by linear and branched bonds.…”

“…The fluorescence lifetime is τ, Boltzmann's constant is k B , and the hydrodynamic volume of the fluorescent molecule is V. Equation ( 2) explicitly indicates that FA is a temperature-sensitive parameter. (11,18) It can also be confirmed that FA depends on the sample fluorescence lifetime, molecular size, and viscosity. In this study, we compared the temperature sensitivity of FA by changing the molecular size as a probe-dependent parameter and the viscosity as a solvent-dependent parameter.…”

“…(16,17) Recently, the important effect of the temperature-dependent viscosity on the rotational correlation time has been reported. (18) However, a detailed investigation of the temperature sensitivity of FA, similarly to that of the temperature coefficient, has not yet been performed. To develop FA-based temperature measurement as a promising method for liquid thermometry, we consider that guidelines for the selection and development of probe molecules are necessary.…”

A Simple Method of Plant Sectioning Using Agarose Embedding Technique for Screening Intracellular Green Fluorescent Protein

“…Many cryoprotectants have been used for cryohistological: glycerol (Kitin et al 2020), su crose (Lestari et al 2018), dimethyl sulfocide (DMSO) (Bekheet et al 2020), polyvinylpyrrolidone (PVP) (Soon thornkalump et al 2020), and polyethylene glycol (PEG) (Roqueborda et al 2021). However, Kaur et al (2022) reported that the steady state fluorescence anisotropy of GFP is about 10-70 °C.…”