We propose an experiment using optically trapped and cooled dielectric microspheres for the detection of short-range forces. The center-of-mass motion of a microsphere trapped in vacuum can experience extremely low dissipation and quality factors of 10 12 , leading to yoctonewton force sensitivity. Trapping the sphere in an optical field enables positioning at less than 1 µm from a surface, a regime where exotic new forces may exist. We expect that the proposed system could advance the search for non-Newtonian gravity forces via an enhanced sensitivity of 10 5 − 10 7 over current experiments at the 1 µm length scale. Moreover, our system may be useful for characterizing other short-range physics such as Casimir forces.PACS numbers: 04.80. Cc,07.10.Pz,42.50.Pq Since the pioneering work of Ashkin and coworkers in the 1970s [1], optical trapping of dielectric objects has become an extraordinarily rich area of research. Optical tweezers are used extensively in biophysics to study and manipulate the dynamics of single molecules, and in soft condensed-matter physics to study macromolecular interactions [2,3]. Much recent work has focused on trapping in solution where strong viscous damping dominates particle motion. There has also been interest in extending the regime that Ashkin and coworkers opened, namely, trapping sub-wavelength particles in vacuum where particle motion is strongly decoupled from a room-temperature environment [1,4].Recent theoretical studies have suggested that nanoscale dielectric objects trapped in ultrahigh vacuum might be cooled to their ground state of (center-of-mass) motion via radiation pressure forces of an optical cavity [5,6]. This remarkable result is made possible by isolation from the thermal bath, robust decoupling from internal vibrations, and lack of a clamping mechanism. In fact, a trapped dielectric nanosphere has been predicted to attain an ultrahigh mechanical quality factor Q exceeding 10 12 for the center-of-mass mode, limited by background gas collisions. Such large Q factors enable cavity cooling, for which the lowest possible phonon occupation of the mechanical oscillator is n T /Q, where n T is the number of room-temperature thermal phonons. Although such Q factors have yet to be observed in experiment, optically levitated microspheres have been trapped in vacuum for lifetimes exceeding 1000 s [1] and electrically levitated microspheres have exhibited pressurelimited damping that is consistent with theoretical predictions down to ∼ 10 −6 Torr [7].In addition to being beneficial for ground-state cooling and studies of quantum coherence in mesoscopic systems, mechanical oscillators with high quality factors also enable sensitive resonant force detection [8,9]. Optically levitated microspheres in vacuum have been studied theoretically in the context of reaching and exceeding the standard quantum limit of position measurement [10]. In this paper, we discuss the force sensing capability of a microsphere trapped inside a medium-finesse optical cavity at ultra-high vacuum, ...