Paper presented is focused on the operating measurements of a tractor wet disc brake system. Operating measurements were evaluated after tractor’s operation at 500 Mth on the 3rd, 4th and 5th gear. Reference fluid and ecological fluid were tested during operation. In addition, work results include the evaluation of the fluid samples taken during the operational tests to monitor the tractor braking performance with wet disc brakes. Fluid samples were also tested in order to determine the changes in their physico-chemical properties. Chemical analysis was performed for both oil samples by means of X-ray fluorescence analysis according to the DIN 51829 and DIN 51399-2. Observed physical properties of the tested oils – density, viscosity, acid number, water content – were within the appropriate ranges after the end of test. Further analysis was focused on an amount of abrasive metals, contaminants, additives, and other important elements. On the basis of analyses conducted, it can be concluded that majority of elements preserved their original level showed at the 0 reference hour sample. In terms of the abrasion metals, an increase in their presence was not found. Furthermore, analysis of the physical properties of tested fluids did not prove their negative impact on the tractor wet disc brake system. Results of the operating measurements suggest that neither the applied conference fluid nor the ecological fluid showed negative effect on the minimum braking value. The minimum braking deceleration was implemented in accordance with the Law no. 106/2018.