Pressurized liquid extraction and anticholinesterase activity-based thin-layer chromatography with bioautography of Amaryllidaceae alkaloids

Mroczek, Tomasz; Mazurek, Jacek M.

doi:10.1016/j.aca.2008.11.053

Cited by 59 publications

(39 citation statements)

References 21 publications

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“…PLE offers many advantages over other extraction techniques including the possibility of automation, low solvent volume and reduced extraction times. In view of the above advantages of PLE, it has been drawing much more attention in the extraction and separation fields [17][18][19].…”

Section: Introductionmentioning

confidence: 99%

Pressurized liquid extraction followed by LC‐ESI/MS for analysis of four chromones in Radix Saposhnikoviae

Wang

Chen

et al. 2010

J of Separation Science

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An efficient pressurized liquid extraction (PLE) technique was employed in extracting chromones from the roots of Saposhnikovia divaricata (Radix Saposhnikoviae). Chromones were quantified and analyzed by LC-ESI/MS. The PLE procedure was optimized, validated and compared with the other conventional extraction techniques. PLE gained the best result due to the highest extraction efficiency within the shortest extraction time. The optimal conditions of PLE were employing 50% ethanol as the extraction solvent at a temperature of 140°C and an extraction pressure of 1500 psi, using one extraction cycle with a static extraction time of 8 min. A good LC separation was achieved using a Hypersil ODS2 column and methanol/water as the mobile phase at a flow rate of 0.8 mL/min. MS coupling with an ESI interface in the positive ion mode was used as the detection technique. This is the first report on combining PLE with LC-ESI/MS for the extraction and quantification of chromones in Radix Saposhnikoviae. The elaborated PLE method also provided a good alternative for the chromone extraction from other plant substances.

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Section: Introductionmentioning

confidence: 99%

Pressurized liquid extraction followed by LC‐ESI/MS for analysis of four chromones in Radix Saposhnikoviae

Wang

Chen

et al. 2010

J of Separation Science

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“…Luthria (2008) showed temperature, pressure, particle size, flush volume, static time, and solid-to-solvent ratio parameters have influence on the extraction of phenolic compounds from parsley (Petroselinum crispum) flakes by PLE. PLE was optimized for extraction of lycorine and galanthamine (Amaryllidaceae alkaloids) from Narcissus jonquilla and an optimized PLE method was more effective than hot-solvent extraction, MAE, and UAE (Mroczek and Mazurek, 2009). Individual phenolic compounds such as gallocatechin (GCT), catechin, epicatechin gallate, caffeic acid, chlorogenic acid, and myricetin and total phenolic contents were recovered from various parts of Anatolia propolis using PLE at optimum condition (40°C, 1500 psi for 15 min) (Erdogan et al, 2011).…”

Section: Pressurized Liquid Extraction (Ple)mentioning

confidence: 99%

Techniques for extraction of bioactive compounds from plant materials: A review

Azmir

Zaidul

Rahman

et al. 2013

Journal of Food Engineering

2,099

1,179

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“…Furthermore, the separation and purification of a certain alkaloid from a crude extraction solution is a very complicated process. At present, there are four kinds of methods basically for the separation and purification of alkaloids: organic solvent extraction, resin adsorption, chromatographic separation methods and high-speed counter-current chromatography (HSCCC) [9][10][11][12][13][14]. The organic solvent extraction method has obvious drawbacks, and it not only is a large volume of organic solvent-dissipative, but also causes environmental problems.…”

Section: Introductionmentioning

confidence: 99%

Preparation and recognition performance of cytisine alkaloid‐imprinted material prepared using novel surface molecular imprinting technique

Gao

Niu

2010

J of Separation Science

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Methacrylic acid was first graft-polymerized on the surfaces of micron-sized silica gel particles in the manner of "grafting from" using 3-methacryloxypropyl trimethoxysilane as an intermedia, obtaining the grafted particle polymethacrylic acid PMAA/SiO(2). By adopting the novel surface-molecular imprinting technique put forward by us, cytisine molecule-imprinted material MIP-PMAA/SiO(2) was prepared with ethylene glycol diglycidyl ether as crosslinking agent. The binding characteristics of MIP-PMAA/SiO(2) towards cytisine was investigated in depth with both batch and column methods and using matrine and oxymatrine as two contrast alkaloids, which with cytisine coexist in sophora alopecuroides and their chemical structure is similar to cytisine to a certain extent. The experimental results show that the surface-imprinted material MIP-PMAA/SiO(2) has excellent binding affinity for cytisine (20.1 g/100 g of binding capacity), and it is more important that MIP-PMAA/SiO(2) has very high recognition selectivity for cytisine in relation to the two contrast alkaloids. The selectivity coefficients of the grafted particles PMAA/SiO(2) (non-imprinted material) for cytosine in relation to matrine and oxymatrine are only 1.03 and 1.06, respectively, displaying no recognition selectivity for cytisine. However, after imprinting, the selectivity coefficient of MIP-PMAA/SiO(2) for cytisine in respect to matrine and oxymatrine are remarkably enhanced to 12.08 and 15.05, respectively.

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Pressurized liquid extraction and anticholinesterase activity-based thin-layer chromatography with bioautography of Amaryllidaceae alkaloids

Cited by 59 publications

References 21 publications

Pressurized liquid extraction followed by LC‐ESI/MS for analysis of four chromones in Radix Saposhnikoviae

Pressurized liquid extraction followed by LC‐ESI/MS for analysis of four chromones in Radix Saposhnikoviae

Techniques for extraction of bioactive compounds from plant materials: A review

Preparation and recognition performance of cytisine alkaloid‐imprinted material prepared using novel surface molecular imprinting technique

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