Prestin-Based Outer Hair Cell Motility Is Necessary for Mammalian Cochlear Amplification

Dallos, Peter; Wu, Xudong; Cheatham, Mary Ann; Gao, Jiangang; Zheng, Jing; Anderson, Charles T.; Song, Jia; Wei, Xiang; Cheng, Wendy H.Y.; Sengupta, Soma; He, David Z.Z.; Zuo, Jian

doi:10.1016/j.neuron.2008.02.028

Cited by 349 publications

(364 citation statements)

References 52 publications

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“…If SFOAEs can be used to assay frequency selectivity, they would provide a more tractable approach for characterizing stimulus coding independent of cochlear location. Preliminary data are provided for wildtype and prestin V499G/Y501H KI mice (referred to here simply as 499) expressing a mutant prestin and showing reduced sensitivity [8]. The SFOAE data from 499 KI mice show loss of sensitivity and no frequency selectivity consistent with previous reports using [2,7], this approach allows us to study the BM-OHC-TM system in young mutant mice and their controls.…”

Section: Introductionsupporting

confidence: 74%

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Using Stimulus Frequency Emissions to Characterize Cochlear Function in Mice

Cheatham¹,

Katz²,

Charaziak³

et al. 2011

AIP Conference Proceedings

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Section: Introductionsupporting

confidence: 74%

“…Recordings were made in wildtype and prestin 499 KI mice [8] bred on site. All animals were anesthetized with ketamine/xylazine and rectal temperature was maintained at ∼38°C using a heating blanket.…”

Section: Methodsmentioning

confidence: 99%

Using Stimulus Frequency Emissions to Characterize Cochlear Function in Mice

Cheatham¹,

Katz²,

Charaziak³

et al. 2011

AIP Conference Proceedings

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“…Post hoc t tests also showed that Otoa KOs with high-frequency SOAEs (average SOAE frequency 18.4 kHz) were statistically different (p G 0.01) from KOs with only low-frequency SOAEs at f2 = 17.8 kHz. For comparison, we appended our results from mice lacking prestin to show highlevel OAEs measured in a cochlea where the outer hair cells lack somatic electromotility and, hence, amplification (Dallos et al 2008). The data were obtained from Prestin KOs that produce DPOAEs since emissions in mice lacking prestin are known to be highly variable such that only those with the best sensitivity generate DPOAEs and then only at high stimulus levels (Liberman et al 2004).…”

Section: Resultsmentioning

confidence: 99%

Increased Spontaneous Otoacoustic Emissions in Mice with a Detached Tectorial Membrane

Cheatham¹,

Ahmad²,

Zhou³

et al. 2015

JARO

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Mutations in genes encoding tectorial membrane (TM) proteins are a significant cause of human hereditary hearing loss , and several mouse models have been developed to study the functional significance of this accessory structure in the mammalian cochlea. In this study, we use otoacoustic emissions (OAE), signals obtained from the ear canal that provide a measure of cochlear function, to characterize a mouse in which the TM is detached from the spiral limbus due to an absence of otoancorin (Otoa, Lukashkin et al. 2012). Our results demonstrate that spontaneous emissions (SOAE), sounds produced in the cochlea without stimulation, increase dramatically in mice with detached TMs even though their hearing sensitivity is reduced. This behavior is unusual because wild-type (WT) controls are rarely spontaneous emitters. SOAEs in mice lacking Otoa predominate around 7 kHz, which is much lower than in either WT animals when they generate SOAEs or in mutant mice in which the TM protein Ceacam16 is absent (Cheatham et al. 2014). Although both mutants lack Hensen's stripe, loss of this TM feature is only observed in regions coding frequencies greater than~15 kHz in WT mice so its loss cannot explain the low-frequency, de novo SOAEs observed in mice lacking Otoa. The fact that~80 % of mice lacking Otoa produce SOAEs even when they generate smaller distortion product OAEs suggests that the active process is still functioning in these mutants but the system(s) involved have become less stable due to alterations in TM structure.

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“…One group of efferents, the medial olivocochlear (MOC) efferents, innervates outer hair cells (OHCs) and controls the gain of mechanical amplification within the cochlea (Cooper and Guinan 2006). Cochlear amplification is produced by OHC receptor currents causing audiofrequency changes in the length of OHCs that amplify cochlear mechanical responses to sound (Dallos et al 2008). MOC fibers synapse on OHCs and produce an OHC hyperpolarization that reduces the effect of Electronic supplementary material The online version of this article (doi:10.1007/s10162-009-0163-1) contains supplementary material, which is available to authorized users.…”

Section: Introductionmentioning

confidence: 99%

Human Medial Olivocochlear Reflex: Effects as Functions of Contralateral, Ipsilateral, and Bilateral Elicitor Bandwidths

Lilaonitkul

Guinan

2009

JARO

111

100

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Animal studies have led to the view that the acoustic medial olivocochlear (MOC) efferent reflex provides sharply tuned frequency-specific feedback that inhibits cochlear amplification. To determine if MOC activation is indeed narrow band, we measured the MOC effects in humans elicited by 60-dB sound pressure level (SPL) contralateral, ipsilateral, and bilateral noise bands as a function of noise bandwidth from 1/2 to 6.7 octaves. MOC effects were quantified by the change in stimulus frequency otoacoustic emissions from 40 dB SPL probe tones near 0.5, 1, and 4 kHz. In a second experiment, the noise bands were centered 2 octaves below probe frequencies near 1 and 4 kHz. In all cases, the MOC effects increased as elicitor bandwidth increased, with the effect saturating at about 4 octaves. Generally, the MOC effects increased as the probe frequency decreased, opposite expectations based on MOC innervation density in the cochlea. Bilateral-elicitor effects were always the largest. The ratio of ipsilateral/contralateral effects depended on elicitor bandwidth; the ratio was large for narrow-band probe-centered elicitors and approximately unity for wide-band elicitors. In another experiment, the MOC effects from increasing elicitor bandwidths were calculated from measurements of the MOC effects from adjacent half-octave noise bands. The predicted bandwidth function agreed well with the measured bandwidth function for contralateral elicitors, but overestimated it for ipsilateral and bilateral elicitors. Overall, the results indicate that (1) the MOC reflexes integrate excitation from almost the entire cochlear length, (2) as elicitor bandwidth is increased, the excitation from newly stimulated cochlear regions more than overcomes the reduced excitation at frequencies in the center of the elicitor band, and (3) contralateral, ipsilateral, and bilateral elicitors show MOC reflex spatial summation over most of the cochlea, but ipsilateral spatial summation is less additive than contralateral.

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Prestin-Based Outer Hair Cell Motility Is Necessary for Mammalian Cochlear Amplification

Cited by 349 publications

References 52 publications

Using Stimulus Frequency Emissions to Characterize Cochlear Function in Mice

Using Stimulus Frequency Emissions to Characterize Cochlear Function in Mice

Increased Spontaneous Otoacoustic Emissions in Mice with a Detached Tectorial Membrane

Human Medial Olivocochlear Reflex: Effects as Functions of Contralateral, Ipsilateral, and Bilateral Elicitor Bandwidths

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