Prestress loss evaluation in prestressed strands is essential for prestressed structures. However, the sensors installed outside the duct can only measure the total prestress loss. The sensors attached on strands inside the duct also have several problems, such as inadequate durability in an aggressive environment and vulnerability to damage during tensioning. This paper proposes a new installation method for long-gauge fiber Bragg grating (LFBG) sensors to prevent accidental damage. Then the itemized prestress losses were determined in each stage of the pre-tensioning and post-tensioning according to the LFBG measurements. We verified the applicability of the LFBG sensors for prestress monitoring and the accuracy of the proposed prestress loss calculation method during pre-tensioning and post-tensioning. In the pre-tensioning case, the calculated prestress losses had less deviation from the true losses than those obtained from foil-strain gauges, and the durability of the LFBG sensors was better than foil-strain gauges, whereas in post-tensioning case, the calculated prestress losses were close to those derived from theoretical predictions. Finally, we monitored prestress variation in the strand for 90 days. The itemized prestress losses at each stages of post-tensioning were obtained by the proposed calculation method to show the prospect of the LFBG sensors in practical evaluation.