Presumptive identification of enteroviruses with RD, HEp-2, and RMK cell lines

Johnston, Sheryl L.G.; Siegel, Charles S.

doi:10.1128/jcm.28.5.1049-1050.1990

Cited by 40 publications

(18 citation statements)

References 7 publications

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“…To select for wild-type poliovirus, HEp-2 cells, which are highly permissive for polioviruses but much less so for other enteroviruses (18,38), were incubated for 5 days with 5% CO 2 at 40°C, a temperature which selects against growth of vaccine poliovirus (24). Tubes showing cytopathic effect were then subpassaged in BGM cultures at 37°C and 5% CO 2 to obtain high-titer viral stocks for characterization by microneutralization assays.…”

Section: Methodsmentioning

confidence: 99%

Detection of Poliovirus Circulation by Environmental Surveillance in the Absence of Clinical Cases in Israel and the Palestinian Authority

et al. 1999

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The global eradication of poliomyelitis, believed to be achievable around the year 2000, relies on strategies which include high routine immunization coverage and mass vaccination campaigns, along with continuous monitoring of wild-type virus circulation by using the laboratory-based acute flaccid paralysis (AFP) surveillance. Israel and the Palestinian Authority are located in a geographical region in which poliovirus is still endemic but have been free of poliomyelitis since 1988 as a result of intensive immunization programs and mass vaccination campaigns. To monitor the wild-type virus circulation, environmental surveillance of sewage samples collected monthly from 25 to 30 sites across the country was implemented in 1989 and AFP surveillance began in 1994. The sewage samples were processed in the laboratory with a double-selective tissue culture system, which enabled economical processing of large number of samples. Between 1989 and 1997, 2,294 samples were processed, and wild-type poliovirus was isolated from 17 of them in four clusters, termed “silent outbreaks,” in September 1990 (type 3), between May and September 1991 (type 1), between October 1994 and June 1995 (type 1), and in December 1996 (type 1). Fifteen of the 17 positive samples were collected in the Gaza Strip, 1 was collected in the West Bank, and 1 was collected in the Israeli city of Ashdod, located close to the Gaza Strip. The AFP surveillance system failed to detect the circulating wild-type viruses. These findings further emphasize the important role that environmental surveillance can play in monitoring the eradication of polioviruses.

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Section: Methodsmentioning

confidence: 99%

Detection of Poliovirus Circulation by Environmental Surveillance in the Absence of Clinical Cases in Israel and the Palestinian Authority

et al. 1999

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“…Retrospective evaluation of the relative utilities of different clinical specimens has proven that stool should be cultured for all patients for NPEV infection. 15 EVs grow well on HeLa cells, HEp-2 cells, rhabdomyosarcoma (RD) cells, MRC-5 cells, human embryonic kidney cells and buffalo green monkey kidney cells, 16 but the RD cell line was found to be the most sensitive cell line for the isolation of EV. 17 NPEV serotype identification can be done by reference antisera.…”

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confidence: 99%

Non‐polio enteroviruses in acute flaccid paralysis children of India: Vital assessment before polio eradication

Dhole

Ayyagarí

Chowdhary

et al. 2009

J Paediatrics Child Health

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“…In the case of coxsackieviruses, some serotypes in group A (A1, A19, and A22) depend absolutely on inoculation of suckling mice for isolation [Schmidt et al, 1975], but members of this group can be isolated in primary monkey kidney cells, human embryonic ®broblasts, or RD cells [Lee et al, 1965;Wecker and Ter Meulen, 1977; , 1978]. Coxsackie B viruses are more readily grown in cell cultures, and Johnston and Siegel [1990] proposed a rapid identi®cation based on growth in primary monkey kidney and HEp-2 cells but not RD cells. However, primary monkey kidney cells are expensive or unavailable to many laboratories, and Hep-2 and RD cells are dif®cult to manage due to rapid growth and deterioration.…”

Section: Discussionmentioning

confidence: 99%

A‐549 is a suitable cell line for primary isolation of coxsackie B viruses

Otero

Folgueira

Trallero

et al. 2001

Journal of Medical Virology

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A common receptor for coxsackie B virus and adenovirus has been described recently in cells of human and murine origin. Since the established cell line A-549 is suitable for adenoviruses, the potential use of A-549 cells for the isolation of coxsackie B viruses from clinical samples was investigated. All throat swabs sent to the laboratory between April 1998 and June 1999 were inoculated onto monolayers of MRC-5 and A-549 cells in tubes, and the enterovirus isolates obtained were typed. From April to June 1999, A-549 cells were compared prospectively to Buffalo green monkey (BGM) cells, considered as the most susceptible cell line for isolating coxsackie B viruses. Fifty-six out of 171 enterovirus isolates (33%) displayed a cytopathic effect (CPE) in the A-549 monolayer only, 48 isolates (28%) in the MRC-5 monolayer only, and 67 isolates (39%) in both cell lines. Most isolates that showed CPE in A-549 cells only (48 out of 56, 86%) were coxsackie B viruses, belonging to four different serotypes (B1, B2, B4, and B6). When BGM and A-549 cells were inoculated in parallel, both recovered the same number of coxsackie B isolates (n = 20), and the CPE was noted on approximately the same day. In conclusion, growth in A-549 but not MRC-5 cells identified coxsackie B viruses in most cases. A-549 was comparable to BGM for primary isolation of coxsackie B viruses.

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Presumptive identification of enteroviruses with RD, HEp-2, and RMK cell lines

Cited by 40 publications

References 7 publications

Detection of Poliovirus Circulation by Environmental Surveillance in the Absence of Clinical Cases in Israel and the Palestinian Authority

Detection of Poliovirus Circulation by Environmental Surveillance in the Absence of Clinical Cases in Israel and the Palestinian Authority

Non‐polio enteroviruses in acute flaccid paralysis children of India: Vital assessment before polio eradication

A‐549 is a suitable cell line for primary isolation of coxsackie B viruses

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