Presynaptic α2-adrenoceptor modulation of 5-hydroxytryptamine and noradrenaline release from vascular adrenergic nerves

Kawasaki, Hiromu; Urabe, Masanobu; Takasaki, Koichiro

doi:10.1016/0014-2999(89)90228-8

Cited by 13 publications

(8 citation statements)

References 8 publications

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“…These results are consistent with the notion that the modulatory adrenoceptors on postganglionic sympathetic nerves are predominantly of the a2 type as has been reported for other systems (Starke, 1987;Lipscombe et al, 1989;Schofield, 1990;Brock et al, 1990). It is interesting to note that in a number of studies, including the present one, yohimbine was unable to antagonize fully the effects of noradrenaline, even at very high concentrations (Kawasaki et al, 1989;Lipscombe et al, 1989). The reduced sensitivity to yohimbine in the present study suggests a similarity of the receptor to the M2D subtype (Simonneaux et al, 1991;Limberger et al, 1992).…”

Section: Effects Ofpertussis Toxin On Inhibition Of K+-evoked Overflosupporting

confidence: 94%

“…The reduced sensitivity to yohimbine in the present study suggests a similarity of the receptor to the M2D subtype (Simonneaux et al, 1991;Limberger et al, 1992). Alternatively, the results may suggest the involvement of more than one x-adrenoceptor subtype as has been previously postulated (Docherty, 1983;Hicks et al, 1986;Daly et al, 1988;Kawasaki et al, 1989;Murphy & Majewski, 1989;Harsing & Vizi, 1991;Wilson & Minneman, 1991;Rump et al, 1992).…”

Section: Effects Ofpertussis Toxin On Inhibition Of K+-evoked Overflosupporting

confidence: 66%

“…Other studies have suggested presynaptic receptors which have properties that do not conform precisely with those of either al-or M2-adrenoceptor subtypes (Elliott et al, 1989;Kawasaki et al, 1989;Lipscombe et al, 1989;Harsing & Vizi, 1991).…”

Section: Introductionmentioning

confidence: 98%

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Involvement of pertussis toxin‐sensitive and ‐insensitive mechanisms in α‐adrenoceptor modulation of noradrenaline release from rat sympathetic neurones in tissue culture

Hill

Powis

Hendry

1993

British J Pharmacology

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1 Sympathetic neurones derived from superior cervical ganglia of neonatal rats and maintained in tissue culture were used to investigate the modulation of neurotransmitter release by presynaptic receptors. Three week old cultures of neurones were loaded with [3H]-noradrenaline to label endogenous neurotransmitter stores. Release of noradrenaline was evoked by depolarization with raised extracellular K+ in the presence of desipramine and corticosterone to prevent uptake of released catecholamine. 2 Potassium (55 mmol 1-) depolarization for 30 s caused more than a four fold increase in 3H overflow from basal levels but this increase was reduced by up to 40% in the presence of exogenous noradrenaline (1 timol 1`). The inhibition by noradrenaline of depolarization-evoked overflow was blocked by the aI/a2-adrenoceptor antagonist, phentolamine. Phentolamine alone did not increase K+-evoked 3H overflow. 3 The a2-adrenoceptor antagonist, yohimbine, produced a concentration-dependent block of the inhibition by noradrenaline of K+-evoked overflow, while the ax-adrenoceptor antagonist, prazosin, was without effect at concentrations up to 0.1 Lmol 1'. 4 The P-adrenoceptor antagonist, propranolol, neither reduced K+-evoked overflow nor increased the degree of inhibition caused by the addition of 1 1tmol 1' noradrenaline.5 The M2-adrenoceptor agonist, clonidine (I iLmol 11) was less effective than noradrenaline at inhibiting K+-evoked overflow, while the a,-adrenoceptor agonist, phenylephrine (1,umol 1I) had no significant effect.6 The L-channel calcium blocker, nicardipine (1 timol 1-') significantly inhibited 3H overflow evoked by K+. In the presence of L-channel block, however, noradrenaline still inhibited residual evoked overflow. 7 In the presence or absence of nicardipine, pertussis toxin pretreatment (1 nmol 1-') reduced, but did not prevent, the effect of noradrenaline (1 tLmol 1'). Pertussis toxin alone caused a significant enhancement of K+-evoked 3H overflow. 8 The data indicate that on postganglionic neurones of cultured rat sympathetic ganglia there are M2-adrenoceptors that modulate neurotransmitter release, but no functional P-adrenoceptors that mediate an enhancement of transmitter release. The data suggest further that in this preparation the mechanism of a2-adrenoceptor modulation may involve pertussis toxin sensitive and insensitive G-proteins and effects on calcium channels other than L-type.

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Section: Effects Ofpertussis Toxin On Inhibition Of K+-evoked Overflosupporting

confidence: 94%

Section: Effects Ofpertussis Toxin On Inhibition Of K+-evoked Overflosupporting

confidence: 66%

See 1 more Smart Citation

Involvement of pertussis toxin‐sensitive and ‐insensitive mechanisms in α‐adrenoceptor modulation of noradrenaline release from rat sympathetic neurones in tissue culture

Hill

Powis

Hendry

1993

British J Pharmacology

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“…Recently, evidence has been produced that 5-HT exists in and/or can be released from coronary, cerebral and mesenteric arteries of rats and rabbits (Griffith et al, 1982;Cohen, 1985;Gale & Cowen, 1988;Kawasaki et al, 1989). Evidence for 5-HT receptor-mediated vasoconstriction in human skin has recently been published by Coffman & Cohen (1988).…”

Section: Discussionmentioning

confidence: 99%

Inhibitory effect of 5‐hydroxytryptamine on penile erectile function in the rat

Finberg

Vardi

1990

British J Pharmacology

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“…The methodology employed for studies of [ 3 H]-5HT release was essentially the same as that described by Kawasaki, Urabe and Takasaki (1989) in rat mesenteric vascular bed with some modi®cations. Brie¯y, isolated iris-ciliary bodies were incubated in oxygenated Krebs buffer solution containing 1 .…”

Section: [ 3 H]-5-ht Release Studiesmentioning

confidence: 99%

[3H]-Serotonin Release from Bovine Iris-ciliary Body: Pharmacology of Prejunctional Serotonin (5-HT7) Autoreceptors

Harris

Awe

Opere

et al. 2001

Experimental Eye Research

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Presynaptic α2-adrenoceptor modulation of 5-hydroxytryptamine and noradrenaline release from vascular adrenergic nerves

Cited by 13 publications

References 8 publications

Involvement of pertussis toxin‐sensitive and ‐insensitive mechanisms in α‐adrenoceptor modulation of noradrenaline release from rat sympathetic neurones in tissue culture

Involvement of pertussis toxin‐sensitive and ‐insensitive mechanisms in α‐adrenoceptor modulation of noradrenaline release from rat sympathetic neurones in tissue culture

Inhibitory effect of 5‐hydroxytryptamine on penile erectile function in the rat

[3H]-Serotonin Release from Bovine Iris-ciliary Body: Pharmacology of Prejunctional Serotonin (5-HT7) Autoreceptors

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