Pretreatment Of Empty Palm Fruit Bunch For Lignin Degradation

Misson, Mailin; Haron, Roslindawati; Kamaroddin, Mohd Fadhzir Ahmad; Amin, Nor Aishah Saidina

doi:10.11113/jt.v50.178

Cited by 6 publications

(6 citation statements)

References 22 publications

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“…Philippoussis (2009) found that SD contains 37.7-49.5% cellulose, 10.7-25.0% hemicelluloses, and 26.1-29.5% Hemicellulose is a polysaccharide with a lower molecular weight than cellulose. The structural complexity of lignin, with its high molecular weight and insolubility, make its degradation difficult (Misson et al, 2009). This is reflected by the lowest mycelial growth rate in PS+EFB (25:75).…”

Section: Resultsmentioning

confidence: 99%

Cultivation of Flammulina velutipesmushroom using various agro-residues as a fruiting substrate

Harith

Abdullah

Sabaratnam

2014

Pesq. agropec. bras.

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-The objective of this work was to evaluate the feasibility of different agro-residues as a carbon source in the fruiting substrates of Flammulina velutipes mushroom and the effect of supplementation with the nitrogen sources spent brewer's yeast and rice bran. The following fruiting substrates were evaluated: rubber wood sawdust (SD), paddy straw (PS), palm empty fruit bunches (EFB), and palm-pressed fiber (PPF). Cultivation was done on each agro-residue, based on formulations consisting of two substrates at the ratios of 3:1, 1:1, and 1:3. Mycelial growth rate and basidiocarp yield were evaluated. The best fruiting substrates were PS+EFB (25:75), PS+PPF (50:50), and PPF (100), with biological efficiency of 185.09±36.98, 150.89±50.35, and 129.06±14.51%, respectively. No significant effects of supplementation with rice bran and spent yeast were observed on mycelial growth rate and biological efficiency. The cultivation of F. velutipes on oil palm wastes does not require additional nitrogen sources.

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Section: Resultsmentioning

confidence: 99%

Cultivation of Flammulina velutipesmushroom using various agro-residues as a fruiting substrate

Harith

Abdullah

Sabaratnam

2014

Pesq. agropec. bras.

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“…Furthermore, peracetic acid solution has an ability to maintain hemicellulose content on the biomass, therefore the loss of hemicellulose can be reduced (Palamae et al 2014). Moreover, the selection of alkaline peroxide as the pretreatment agent was done as it removes almost all lignin content on the biomass (Misson et al 2009). Alkaline peroxide pretreatment can also improve the performance of enzymatic hydrolysis, since it plays a role in lignin release from the lignocellulose matrix and increases the degree of hydration of the cellulose (Gould 1985).…”

Section: Resultsmentioning

confidence: 99%

Effect of Ultrasound-Assisted on Sequential Peracetic Acid and Alkaline Peroxide Pretreatment to The Enzymatic Hydrolysis of Oil Palm Empty Fruit Bunch

Putri

Perdani²,

Yohda

et al. 2021

Preprint

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Enzymatic hydrolysis of oil palm empty fruit bunch (OPEFB) that has been pretreated by modified pretreatment has been investigated in this study. The OPEFB used was pretreated by using sequential peracetic acid – alkaline peroxide solution. As the modification method, the assistance of pretreatment by ultrasound was conducted, in order to increase the enzyme accessibility. Therefore, it enhances the production of reducing sugar on the hydrolysis process. Prior to hydrolysis process, OPEFB was initially treated by using peracetic acid solution, comprise of CH3COOH (> 99%) and H2O2 (30% w/w), assisted by ultrasound for 3 hours at 35oC. Afterwards, OPEFB was treated by using alkaline peroxide solution, comprise of NaOH (40% w/w) and H2O2 (35% w/w), assisted by ultrasound for 10 hours at 35oC. OPEFB that has been pretreated was then subjected to enzymatic hydrolysis process using cellulase enzyme, in order to convert cellulose content into reducing sugar. Enzymatic hydrolysis was carried out at 50oC in a shaker incubator with 150 rpm for 48 hours. In this study, the effect of different enzyme concentration and hydrolysis time towards the sugar concentration in modified-pretreated OPEFB was observed and analyzed. Three different concentrations of enzyme were used, including 1.25, 2.5, and 5 g/L, and reducing sugar concentrations were analyzed at 30 and 45 minutes, and 1, 2, 4, 6, 24, 30, and 48 hours. Based on results, enzyme concentration has a significant effect to the production of reducing sugar. The reducing sugar concentrations obtained at the end of the hydrolysis process were 8.48, 11.06, 19.16 g/L, at the enzyme concentrations of 1.25, 2.5, and 5 g/L, respectively. At any hydrolysis time, the highest sugar concentration has been achieved on the highest enzyme concentration of 5 g/L. Moreover, the effective hydrolysis time were achieved at 6 hours, at all concentration of enzyme, since the production of reducing sugar were insignificant after 6 hours. This study showed an increase in reducing sugar production by 8.25% in the hydrolysis process using OPEFB pretreated by modified pretreatment compared to the non-modified pretreatment.

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“…Several researchers have succeeded in achieving the delignification of EFB by using chemical treatment. It has been delignified simultaneously and consecutively by using NaOH and H2O2, with lignin removal of 72% and 99% (Misson et al, 2009). Another chemical treatment has been attempted using peracetic acid solution.…”

Section: Introductionmentioning

confidence: 99%

Delignification of Oil Palm Empty Fruit Bunch using Peracetic Acid and Alkaline Peroxide Combined with the Ultrasound

Hermansyah¹,

Putri

Prasetyanto

et al. 2019

IJTech

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Lignocellulosic biomass has great potential as a low-cost source of fermentable sugar for the production of biofuels and high value organic acids. One potential biomass is oil palm empty fruit bunch, since it has high cellulose and hemicellulose content. However, its lignin content can hinder the access of cellulose and hemicellulose during the hydrolysis process. Therefore, an effective pretreatment for the delignification of lignocellulose biomass should be considered to reduce the lignin content. In this study, delignification of oil palm empty fruit bunch using peracetic acid and alkaline peroxide solution combined with the ultrasound method is investigated as a novel combination method of biomass pretreatment. The effect of pretreatment time was observed by using a peracetic acid solution for 1, 3, 5, 7 and 9 hours, followed by an alkaline peroxide solution for 4, 6, 8 and 10 hours. Based on the results, the best delignification was achieved by pretreatment using peracetic acid pretreatment for 3 hours, followed by alkaline peroxide pretreatment for 10 hours. Both pretreatments were assisted by the ultrasound method. The results show hemicellulose, cellulose and lignin content of 14.13%, 77.27% and 8.6% respectively. The lignin content was reduced by 68.73% and the cellulose content increased by 121.85%, relative to the untreated EFB. This result was considered as the best pretreatment, since the pretreatment time was shorter and high cellulose content together with low lignin content was achieved, which will improve the hydrolysis process.

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Pretreatment Of Empty Palm Fruit Bunch For Lignin Degradation

Cited by 6 publications

References 22 publications

Cultivation of Flammulina velutipesmushroom using various agro-residues as a fruiting substrate

Cultivation of Flammulina velutipesmushroom using various agro-residues as a fruiting substrate

Effect of Ultrasound-Assisted on Sequential Peracetic Acid and Alkaline Peroxide Pretreatment to The Enzymatic Hydrolysis of Oil Palm Empty Fruit Bunch

Delignification of Oil Palm Empty Fruit Bunch using Peracetic Acid and Alkaline Peroxide Combined with the Ultrasound

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