Pretreatment of plasma samples by a novel hollow fiber centrifugal ultrafiltrate device for the determination of cefaclor concentrations in human plasma

Li, Junmei; Li, Cen; Jiang, Ye; Ren, Shumeng

doi:10.1016/j.chroma.2010.08.059

Cited by 20 publications

(12 citation statements)

References 29 publications

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“…In this study, a HFCF-UF method was developed to determine the free MTX plasma concentration: accurate and precise results were obtained, and the time and workload required for sample preparation procedure were reduced, because the simple proposed procedure involves a step of ordinary centrifugation that can be easily performed in any laboratory ( Dong et al, 2013a ; Dong et al, 2013b ; Zhang et al, 2013b ; Zhang et al, 2014; Zhang et al, 2015 ). In the proposed method, the membrane was completely parallel to the centrifugal force, and small molecules could freely pass through the membrane without being affected by molecular sieves and concentration polarization ( Li et al, 2010 ; Zhang et al, 2013b ). The volume ratio of the ultrafiltrate to the sample solution was sufficiently small and constant to minimally affect the protein-binding equilibrium ( Dong et al, 2013b ).…”

Section: Discussionmentioning

confidence: 99%

Relationship Between the Free and Total Methotrexate Plasma Concentration in Children and Application to Predict the Toxicity of HD-MTX

Dong

Guo

et al. 2021

Front. Pharmacol.

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High-dose methotrexate (HD-MTX) can be highly effective as well as extremely toxic. Many drug molecules can bind to plasma proteins to different extents in vivo, whereas only the free drug can reach the site of action to exert a pharmacological effect and cause toxicity. However, free MTX concentrations in plasma have not been reported. Traditional analyses of free drugs are both cumbersome and inaccurate. We collected 92 plasma samples from 52 children diagnosed with ALL or NHL or other lymphomas that were treated with HD-MTX. The hollow fiber centrifugal ultrafiltration (HFCF-UF) was used to prepare plasma samples for analysis of the free MTX concentration. Protein precipitation was employed to measure the total MTX concentration. The HFCF-UF is a simple method involving a step of ordinary centrifugation; the validation parameters for the methodological results were satisfactory and fell within the acceptance criteria. A linearity coefficient r2 of 0.910 was obtained for the correlation between the free and total MTX plasma concentrations in 92 plasma samples. However, the free and total MTX concentrations was only weakly correlated in 16 clinical plasma specimens with total MTX concentrations >2 μmol L−1 (r2 = 0.760). Both the free and total MTX concentrations at 42 h were negatively correlated with the creatinine clearance (CCr) level (P = 0.023, r = −0.236 for total MTX and P = 0.020, r = −0.241for free MTX, respectively). The free MTX concentration could not be accurately estimated from the total MTX concentration for patients with high MTX levels which are conditions under which toxic reactions are more likely to occur. High plasma MTX levels could become a predictor of the occurrence of MTX nephrotoxicity to draw people's attention. The proposed HFCF-UF method is a simple and accurate way to evaluate efficacy and toxicity in clinical therapeutic drug monitoring.

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Section: Discussionmentioning

confidence: 99%

Relationship Between the Free and Total Methotrexate Plasma Concentration in Children and Application to Predict the Toxicity of HD-MTX

Dong

Guo

et al. 2021

Front. Pharmacol.

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“…The hollow fiber centrifugal ultrafiltrate (HFCF-UF) product (our patented product, composed of a tube and a u-shaped hollow fiber; project no: 2009100744294) has been used to separate the macro-molecules from complicated matrixes such as plasma (14,15). The HFCF-UF is good for class separation by different molecular weight cut-off, and HPLC enables high-efficiency fractionation; therefore, HFCF-UF-HPLC has been shown to be an effective means in analysis of various components in complex matrixes.…”

Section: Introductionmentioning

confidence: 99%

Application of hollow fiber centrifugal ultrafiltrate purification as the pretreatment technology for traditional Chinese medicine: its application for analysis of honokiol and magnolol in TCM preparations containing Cortex Magnoliae Officinalis

An¹,

He²,

Guo³

et al. 2021

Ann Palliat Med

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Background: Traditional Chinese medicine (TCM) preparations are very complex mixtures, and the content of bioactive components is usually very low. Therefore, before final analysis, the preparation of an appropriate sample is necessary. Sample preparation is the most time-consuming and error-prone part of the analytical procedure, and the choice of purification technology greatly influences the reliability of the final analysis. Methods: In the present study, we evaluated the feasibility of hollow fiber centrifugal ultrafiltrate (HFCF-UF) as a purification technology for the analysis of bioactive components in TCM preparations. The HFCF-UF technology was applied to analyze honokiol and magnolol in TCM preparations containing Cortex Magnoliae Officinalis (Hou Po in Chinese Pinyin). A mini centrifugal device based on hollow fiber was employed to remove the macromolecule components. A single step of simple centrifugation was required before the filtrate could be directly injected into an existing high performance liquid chromatography (HPLC) system without any further clean-up step or use of special columns. This greatly simplified the pretreatment steps, and improved the accuracy of analytic methods. The separation was achieved on a Diamonsil C 18 column (i.d. 5 μm, 150 mm × 4.6 mm) with V (methanol):V (acetonitrile):V (0.5% acetic acid solution) =44:22:34 as the mobile phase at a flow rate of 1.0 mL/min. Results: It had good linear relationship between the peak areas of honokiol and magnolol and their concentrations at 6.40-205 and 3.15-101 μg/mL (r=0.9999), respectively. The method recovery was over 92.6 % with a relative standard deviation (RSD) of less than 3.0%. The average recovery of honokiol was 97.7% with an RSD of 3.0%, and that of magnolol was 96.8% with RSD of 2.8%. Conclusions: The application of HFCF-UF in TCM preparations could assist in making the quality control of TCM simple, rapid, and accurate. The HFCF-UF purification procedure can be used as an alternative means for analyzing bioactive components in TCM preparations.

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“…Low-abundant sample detection remains a major hurdle for rapid diagnostic testing, as the tiny biomolecules are extremely hard to concentrate with filters, centrifuges, or any other current method without huge losses of the aggregated molecules. [1][2][3][4][5] Electrokinetic concentration, also known as ion concentration polarization (ICP), has recently gained attention as a promising alternative to conventional sieving or centrifugal filters for identification of low-abundant ion/molecular analysis. [6][7][8] Most electrokinetic concentrators rely on cation exchange membranes (CEM) such as Nafion that induce intensive electric fields at a two different kinds of nanochannels but those are limited to the microfluidic-scale.…”

Section: Introductionmentioning

confidence: 99%

A Multiscale‐Porous Anion Exchange Membrane for Convenient and Scalable Electrokinetic Concentration of Cationic Species

Lee

Kwon

Lim

2020

Adv Funct Materials

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A device able to electrokinetically concentrate cationic samples has many potential medical and industrial applications, but until now has remained undeveloped due to the lack of a commercial anion‐permselective material leading to a prohibitively complex fabrication procedure. Herein, a novel multiscale‐porous anion exchange membrane (MP‐AEM) that enables the convenient and scalable electrokinetic concentration of cationic species is proposed. A mechanically enhanced multiscale‐porous structure with a solid framework is realized by adopting polyester resin as an additive to overcome the intrinsic limitations of the AEM material. The scalable MP‐AEM‐embedded electrokinetic concentrator is devised based on the peculiar properties of the MP‐AEM that for allow both ion and fluid transport. With the MP‐AEM, the concentrator is fabricated in a highly streamlined manner consisting only of a simple insertion and assembly. The concentration performance of the MP‐AEM‐embedded electrokinetic concentrator is demonstrated with a positively charged fluorescent dye and a fluorescein‐labeled protein, and the results show enrichment factors of 250 and 500, respectively. The MP‐AEM makes cationic electrokinetic concentration more accessible and scalable, thereby enabling further progress in a wide range of fields.

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Pretreatment of plasma samples by a novel hollow fiber centrifugal ultrafiltrate device for the determination of cefaclor concentrations in human plasma

Cited by 20 publications

References 29 publications

Relationship Between the Free and Total Methotrexate Plasma Concentration in Children and Application to Predict the Toxicity of HD-MTX

Relationship Between the Free and Total Methotrexate Plasma Concentration in Children and Application to Predict the Toxicity of HD-MTX

Application of hollow fiber centrifugal ultrafiltrate purification as the pretreatment technology for traditional Chinese medicine: its application for analysis of honokiol and magnolol in TCM preparations containing Cortex Magnoliae Officinalis

A Multiscale‐Porous Anion Exchange Membrane for Convenient and Scalable Electrokinetic Concentration of Cationic Species

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