Prevalence and Antibiogram of S. mutans in Dental Plaque and Caries samples

Abstract

Streptococcus mutans is a Gram-positive facultative anaerobic bacterium that belongs to the mutans streptococci group and is considered to be an important pathogen associated with human dental caries. In this study, 106 S. mutans isolates were isolated from 140 clinical samples. The 106 isolates comprised 56 isolates from soft caries lesion, 50 isolates from dental plaque samples, the latter included 30 isolates from plaque of caries active and 20 isolates from plaque of caries-free cases. The isolates showed sticky and rough colonies on TYCSB media. Isolates produced alpha hemolysis on blood agar. Isolates were catalase-negative and able to ferment mannitol, sorbitol, inulin and sucrose. The identification of S. mutans was confirmed by the detection of the S. mutans-specific glucosyltransferase gene (gtfB) using PCR specific primers for S. mutans gtfB gene. Isolates were tested for their sensitivity to diverse antimicrobial agents. It was found that 99% of the isolates were sensitive to ofloxacin and levofloxacin and 88.5% of isolates was sensitive to chloramphenicol, however, 80.2%, 75.5% and 65% of the isolates were resistant to ceftriaxone, cefotaxime and erythromycin, respectively. It was recorded that 90.5% and 85.8% of the isolates were sensitive to linezolid and tetracycline, respectively, and were highly resistant to clindamycin with a frequency of 95.3%. Based on the statistical analysis using the chi-square test, there was a significant difference in antimicrobial susceptibility profiles among the three groups with higher frequencies among soft caries lesion isolates (P < 0.0001). Therefore, the extensive use of antimicrobials in treatment and prophylaxis in dental caries must be controlled.

Isolation and diagnosis of Streptococcus mutans and Streptococcus sorbinus from patients with tooth decay and gingivitis

“…The same l. et a isolates except that dSOR had a negative result (Table 6). The results of Abo Baker S. mutans results as showed that S. mutans isolated from patients in the Arab Republic of Egypt with tooth decay and decay had the , as well as [30] ferment four main sugars used in their diagnosis: mannitol, sucrose, sorbitol and inulin ability to the two types of bacteria S. mutans and S. sobrinus have the ability to ferment and consume lactose, mannose and raffinose, and the production of dextran of sucrose has an effective role in the pathogenesis of bacteria and [31,32]. the cause of decay.…”

A comparative study of the antimicrobial activity of cacao ethanol extract and chlorhexidine digluconate on salivary Streptococcus mutans

“…coccus bacteria that inhabits the oral cavity (3) . It is one of the foremost dominant disease-causing organisms that plays a role in dental caries.…”