Prevalence and antibiotic resistance of Salmonella Enteritidis and Salmonella Typhimurium in raw chicken meat at retail markets in Malaysia

Thung, Tze Young; Mahyudin, Nor Ainy; Basri, Dayang Fredalina; Radzi, Che Wan Jasimah Wan Mohamed; Nakaguchi, Yoshitsugu; Nishibuchi, Mitsuaki; Radu, Son

doi:10.3382/ps/pew144

Cited by 112 publications

(78 citation statements)

References 24 publications

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“…The majority of publications included data from Thailand (20) [27,28,29,30,31,32,33,34,35,36,37,38,39,40,41,42,43,44] and Vietnam (15) [18,23,45,46,47,48,49,50,51,52,53,54,55]. One publication was found with data from each of Cambodia, Indonesia, Lao PDR and Malaysia [56,57,58,59]. One study investigated imported aquaculture products from several SEA countries [60].…”

Section: Resultsmentioning

confidence: 99%

Antimicrobial Usage and Antimicrobial Resistance in Animal Production in Southeast Asia: A Review

et al. 2016

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Southeast Asia is an area of great economic dynamism. In recent years, it has experienced a rapid rise in the levels of animal product production and consumption. The region is considered to be a hotspot for infectious diseases and antimicrobial resistance (AMR). We reviewed English-language peer-reviewed publications related to antimicrobial usage (AMU) and AMR in animal production, as well as antimicrobial residues in meat and fish from 2000 to 2016, in the region. There is a paucity of data from most countries and for most bacterial pathogens. Most of the published work relates to non-typhoidal Salmonella (NTS), Escherichia coli (E. coli), and Campylobacter spp. (mainly from Vietnam and Thailand), Enterococcus spp. (Malaysia), and methicillin-resistant Staphylococcus aureus (MRSA) (Thailand). However, most studies used the disk diffusion method for antimicrobial susceptibility testing; breakpoints were interpreted using Clinical Standard Laboratory Institute (CSLI) guidelines. Statistical models integrating data from publications on AMR in NTS and E. coli studies show a higher overall prevalence of AMR in pig isolates, and an increase in levels of AMR over the years. AMU studies (mostly from Vietnam) indicate very high usage levels of most types of antimicrobials, including beta-lactams, aminoglycosides, macrolides, and quinolones. This review summarizes information about genetic determinants of resistance, most of which are transferrable (mostly plasmids and integrons). The data in this review provide a benchmark to help focus research and policies on AMU and AMR in the region.

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Section: Resultsmentioning

confidence: 99%

Antimicrobial Usage and Antimicrobial Resistance in Animal Production in Southeast Asia: A Review

et al. 2016

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“…Typhimurium and S . Enteritidis are the common foodborne pathogens detected in Malaysia (Modarressi and Thong, 2010; Nillian et al, 2011; Pui et al, 2011; Adzitey et al, 2012; Najwa et al, 2015; Thung et al, 2016). It is worth noting that E. coli O157:H7 and S .…”

Section: Discussionmentioning

confidence: 99%

Comparison of the Microbiological Quality and Safety between Conventional and Organic Vegetables Sold in Malaysia

et al. 2017

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Given the remarkable increase of public interest in organic food products, it is indeed critical to evaluate the microbiological risk associated with consumption of fresh organic produce. Organic farming practices including the use of animal manures may increase the risk of microbiological contamination as manure can act as a vehicle for transmission of foodborne pathogens. This study aimed to determine and compare the microbiological status between organic and conventional fresh produce at the retail level in Malaysia. A total of 152 organic and conventional vegetables were purchased at retail markets in Malaysia. Samples were analyzed for mesophilic aerobic bacteria, yeasts and molds, and total coliforms using conventional microbiological methods. Combination methods of most probable number-multiplex polymerase chain reaction (MPN-mPCR) were used to detect and quantify foodborne pathogens, including Escherichia coli O157:H7, Shiga toxin-producing E. coli (STEC), Listeria monocytogenes, Salmonella Typhimurium, and Salmonella Enteritidis. Results indicated that most types of organic and conventional vegetables possessed similar microbial count (P > 0.05) of mesophilic aerobic bacteria, yeasts and molds, and total coliforms. E. coli O157:H7 and S. Typhimurium were not detected in any sample analyzed in this study. Among the 152 samples tested, only the conventional lettuce and organic carrot were tested positive for STEC and S. Enteritidis, respectively. L. monocytogenes were more frequently detected in both organic (9.1%) and conventional vegetables (2.7%) as compared to E. coli O157:H7, S. Typhimurium, and S. Enteritidis. Overall, no trend was shown that either organically or conventionally grown vegetables have posed greater microbiological risks. These findings indicated that one particular type of farming practices would not affect the microbiological profiles of fresh produce. Therefore, regardless of farming methods, all vegetables should be subjected to appropriate post-harvest handling practices from farm to fork to ensure the quality and safety of the fresh produce.

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“…There are more than 2600 identified Salmonella serotypes, of which Salmonella enterica serovar Enteritidis ( S . Enteritidis) is the one most frequently associated with human disease (Lee, Runyon, Herrman, Phillips, & Hsieh, ; Santander et al., ; Thung et al., ). Foodborne outbreaks have been increasing due to the consumption of unpasteurized acidic fruit juices, and the use of acidic conditions in food industries to prevent microbial contamination and maintain product quality (Cheng, Churey, & Worobo, ).…”

Section: Introductionmentioning

confidence: 99%

Response to Acid Adaptation in Salmonella enterica Serovar Enteritidis

Zhou

et al. 2019

Journal of Food Science

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Acid adaptation in Salmonella Enteritidis was characterized by phenotypic and gene‐expression analyses. S. Enteritidis cells at log‐phase and stationary‐phase were kept at pH 4.5 to 6.0 for 1 to 4 hours. All treatments induced various levels of acid tolerance response that were dependent on pH, exposure time and growth phase. This acid adaptation resulted in tolerance to 50 °C and 8% NaCl regardless of the growth phase. However, the tolerance of log‐phase and stationary‐phase cells to low temperatures (4 and –20 °C) was increased and decreased, respectively. RT‐qPCR analysis revealed that genes involved in tolerance to acid (SEN1564A and cfa), heat (rpoH, uspB, and htrA), salt (proP, proV, and osmW), and cold (cspA, cspC, and csdA) stress were generally upregulated after acid adaptation. These results provide an initial insight into mechanisms of acid adaptation and induced cross protection in S. Enteritidis. Practical Application Stress tolerance acquisition resulting from acid adaptation in foodborne pathogens poses a great threat to food safety. The current work showed that acid adaptation induced direct tolerance and cross‐tolerance to high temperature, low temperature, and salt in Salmonella Enteritidis, possibly due to the upregulation of stress tolerance‐related genes. These results provide key insights into acid adaptation mechanisms and efficient control of S. Enteritidis.

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Prevalence and antibiotic resistance of Salmonella Enteritidis and Salmonella Typhimurium in raw chicken meat at retail markets in Malaysia

Cited by 112 publications

References 24 publications

Antimicrobial Usage and Antimicrobial Resistance in Animal Production in Southeast Asia: A Review

Antimicrobial Usage and Antimicrobial Resistance in Animal Production in Southeast Asia: A Review

Comparison of the Microbiological Quality and Safety between Conventional and Organic Vegetables Sold in Malaysia

Response to Acid Adaptation in Salmonella enterica Serovar Enteritidis

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