Prevalence and antibiotic susceptibility of Aeromonas hydrophila isolated from freshwater fishes

Abstract

CitationSarder H, Khan T, Saha ML, Punom NJ, Mandal SC and Rahman MS (2016) Prevalence and antibiotic susceptibility of Aeromonas hydrophila isolated from freshwater fishes. Journal of Fisheries 4(3): 411-419. DOI: 10.17017/jfish.v4i3.2016.177 Abstract Aeromonas hydrophila is an opportunistic microorganism. It is a secondary biological agent that contributes to the occurrence of fish diseases and its deterioration. This research was undertaken to determine the prevalence of A. hydrophila in some freshwater fishes collected from three different fish markets of Dhaka City and to test their antibiotic susceptibility. Total bacterial count and total aeromonas on different aeromonas selective media were enumerated using serial dilution technique. Bacterial isolates were characterized to identify A. hydrophila using biochemical tests and with comparison to reference strain (ATCC 7966). The lowest Aeromonas count was detected to be 2.83±0.40×10 2 cfu/g in Anabas testudineus and the highest was 1.03±0.153×10 3 cfu/g in Oreochromis mossambicus. On market basis highest aeromonas count was found in Anando Bazar (8.10±1.09×10 2 cfu/g) and lowest in Hatirpool Bazar (5.63±0.90×10 2 cfu/g) with no significant difference. Maximum susceptibility to amikacin and gentamicin was observed whereas all of the isolates were found resistant to a commonly used antibiotic amoxycillin. The obtained results point that antimicrobial susceptibility was more or less similar regardless of the origin of the samples collected. All the fishes investigated in this study contained A. hydrophila in their different organs.

Fungal and Bacterial Co-Infection of Sea Bass (Dicentrarchus labrax, Linnaeus 1758) in a Recirculating Aquaculture System: Saprolegnia parasitica and Aeromonas hydrophila

“…Aeromonas hydrophila has been reported in Salmo gairdneri (Peters et al, 1988), Carassius auratus (Citarasu et al, 2011), Oreochromis niloticus (Ibrahem et al, 2008), Clarias batrachus (Angka, 1990), Cyprinus carpio (Citarasu et al, 2011) with skin lesions and hemorrhagic septicemia over the body, tail and fin regions (Sarder et al, 2016). It is generally considered to be a secondary invader (Rogers, 1971) like Saprolegniosis.…”

Monitoring some pathogenic bacteria in water and fish of Lake Qaroun

“…Infections caused by aeromonad in fish were mostly reported to be associated with A. hydrophila. It is an opportunistic microorganism (Sarder et al, 2016 ). Results in this study pointed out that the highest density of A. hydrophila was recorded in summer followed by spring.…”

Isolation and Characterization of Multiple Antibiotic Resistant A. hydrophila causing Mortality of Indian Major Carps in Ganjam District, Odisha

“…The findings of this study match well with the previous reports that Aeromonas hydrophila isolated from various fresh water fishes from Syria, Bangladesh and Rajasthan were 100% susceptible to Ciprofloxacin (Daood, 2011;Lijon et al, 2015;Monir et al, 2016;Rawal et al, 2016). However, few researchers of different regions have reported varying percentage of resistance for A. hydrophilla isolates towards Ciprofloxacin i.e., 31.75% in South China (Yang et al, 2017), 6.25% at Dhaka in Bangladesh (Sarder et al, 2016) and 30.8% at Odisha and Andhra Pradesh in India (Samal et al, 2014 (Samal et al, 2014), 25% in Bangladesh (Sarder et al, 2016), 87.5% at Rajasthan in India (Rawal et al, 2016) and 79.37% in South China (Yang et al, 2017). On the other hand, our findings as 100% sensitive towards Gentamycin corroborates with many others earlier reports (Lijon et al, 2015;Monir et al, 2016;Rawal et al, 2016;Sarder et al, 2016;Yang et al, 2017).…”

“…Antimicrobial drug therapy is the most widely used method of control of Aeromoniasis in fish culture farms (Guz and Kozińska, 2004). However, the main problem in the use of antibiotics against Aeromonas infection is the development of resistance by these bacteria to these antibiotics (Sarder et al, 2016). Besides, the massive use of antimicrobials at sub-optimal doses as growth promoters in aquaculture farms encourages the natural emergence of bacterial resistance towards the antimicrobials (Chu and Zhu, 2010;FDA, 1998).…”

“…to commonly used antibiotics is an emerging problem in aquaculture. Repeated reports on multidrug resistance in the genus Aeromonas is a great concern today (Albert et al, 2000;Vivekanandhan et al, 2002;Palu et al, 2006;Lijon et al, 2015;Sarder et al, 2016), since the increase in resistance increases the difficulty in treating such diseases in fishes. The situation becomes grave when such strain is zoonotic in nature and involved in human infections.…”