Prevalence and antimicrobial profiling of Campylobacter spp. isolated from meats, animal, and human feces in Northern of Morocco

Baaboua, Aicha El; Maadoudi, Mohamed El; Bouyahya, Abdelhakim; Kounnoun, Ayoub; Bougtaib, Hajar; Belmehdi, Omar; Senhaji, Nadia Skali; Abrini, Jamal

doi:10.1016/j.ijfoodmicro.2021.109202

Cited by 12 publications

(12 citation statements)

References 45 publications

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“…Tetracycline has been used for animal production for many years, and this study also showed high tetracycline resistance in all species of Campylobacter isolates. The resistance to tetracycline in Campylobacter species might be due to presence of plasmid-encoded tet (O) resistant gene in both C. jejuni and C. coli [38,39]. Campylobacter species isolates in this study were also highly resistant to Cephalothin, Ampicillin and cefotaxime.…”

Section: Plos Onementioning

confidence: 63%

“…However, Campylobacter species in this study were highly resistant for chloramphenicol and Tetracycline. Studies conducted in Morocco also documented resistant strains of Campylobacter species for tetracycline and other regimen of antibiotics [39,40]. Other studies also reported chloramphenicol and Tetracycline resistant Campylobacter species [16,41].…”

Section: Plos Onementioning

confidence: 93%

“…In recent years, increased resistance was observed for fluoroquinolones, macrolides, aminoglycosides, and beta lactams [35]. Furthermore, intrinsic resistance in C. jejuni and C. coli has been described against penicillins, most of the cephalosporin as well as trimethoprim, sulfamethoxazole, rifampicin, and vancomycin [36][37][38][39]. Campylobacter isolates in the present study were susceptible to Nalidixic acid (86.8%), ciprofloxacin (86.8%), Sulphamethazole (84.2%) and ceftriaxone (78.9%).…”

Section: Plos Onementioning

confidence: 99%

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Isolation, identification and antibiotic resistance profile of thermophilic Campylobacter species from Bovine, Knives and personnel at Jimma Town Abattoir, Ethiopia

Debelo

Mohammed²,

Tiruneh³

et al. 2022

PLoS ONE

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Thermophilic Campylobacter species are common cause of animal and human bacterial diseases with growing resistance to antimicrobials. The aim of this study was to determine the prevalence and antimicrobial susceptibility pattern of Campylobacter species from bovine, knives and personnel in Jimma Town, Ethiopia. Faecal samples and carcasses swabs were collected from cattle systematically selected from the annual plan of Jimma Municipal Abattoir. Personnel hand and knife swabs were collected after slaughtering each selected cattle. A cross-sectional study with systematic sampling method was conducted from October 2019 to September 2020 for the isolation, identification and antimicrobial susceptibility pattern of thermophilic Campylobacter species. Isolation and identification of Campylobacter species were performed according to the techniques recommended by the International Organization for Standardization, and in vitro antibiotic susceptibility testing was screened using the standard agar disc diffusion method as recommended by Clinical and Laboratory Standards Institutions. A total of 684 samples (171 samples from faeces, carcasses, knives and personnel hands, were collected independently). The overall prevalence of thermophilic Campylobacterspecies was 5.6% (38/684). Majority of the isolates were from faecal samples (12.9%, n = 22) followed by carcass swabs(4.1% n = 7), knife swabs(3.5% n = 6) and personnel hand swabs(1.8% n = 3). Isolated and identified species of C.jejuni, C. coli and C. lari accounted for 63.2%, 23.7% and 13.2%, respectively. The isolated Campylobacter species were found to be resistant to Cephalothin (100%), Ampicillin (60.5%), Cefotaxime (60.5%), Chloramphenicol (47.4%) and Tetracycline (42.1%). On the other hand, the isolates were susceptible to Nalidixic acid (86.8%), Ciprofloxacin (86.8%), Sulphamethazole (84.2%), Ceftriaxone (78.9%), Clindamycin (68.4%) and Cefixime (65.8%). 84.2% of the isolates showed multi-drug resistance for three-to-six drug classes. All the C. lari isolates were multidrug resistant. All the three isolated species of Campylobacter were resistant to Cephalothin, and most were multidrug resistant. Isolation of Campylobacter species from faecal, carcass, knife and hand swabs revealed possible risk of contamination and exposure to Campylobacter infection of those who consume raw meat. Therefore, enactment of hygienic practices during the slaughtering process, proper handling and cooking of meat and awareness creation on jurisdictional antibiotic usage are required to avoid Campylobacter infection.

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Section: Plos Onementioning

confidence: 63%

Section: Plos Onementioning

confidence: 93%

Section: Plos Onementioning

confidence: 99%

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Isolation, identification and antibiotic resistance profile of thermophilic Campylobacter species from Bovine, Knives and personnel at Jimma Town Abattoir, Ethiopia

Debelo

Mohammed²,

Tiruneh³

et al. 2022

PLoS ONE

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“…Thus far, real-time PCR is the most sensitive and reliable tool for the detection of Campylobacter genus. In the light of these ndings, we suggested the evaluation of multiplex qPCR to include HipO, CadF, and other genes for an easy and appropriate routine use (El Baaboua et al, 2021). Unlike, the replacement of the culture approach seems to be di cult (Liang et al, 2018;Baaboua et al, 2021).…”

Section: Direct Application Of Qpcr On Positive Enrichment Broths Cam...mentioning

confidence: 99%

“…Chessboard titration for amendment of C. coli qPCR protocol Con rmation of Campylobacter pure isolates From July 2015 to June 2018, 464 of meat samples, in Northern of Morocco, were analyzed according to the horizontal method for detection and enumeration of Campylobacter in foods, following Moroccan standard NM ISO: 10272-1 (2008) with slight modi cations(El Baaboua et al, 2021). 130 suspicious Campylobacter spp.…”

mentioning

confidence: 99%

Development and Evaluation of Real Time PCR for Detection and Identification of C. jejuni and C. coli from Human Food

Baaboua

Maadoudi²,

Belmehdi

et al. 2022

Preprint

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The current study aimed to develop, optimize, and evaluate the real-time polymerase chain reaction (qPCR) specificity and sensitivity on pure isolated strains of Campylobacter and on enriched broth for direct detection of these cells. To achieve this, HipO and CadF gene were purchased to target C. jejuni and C. coli, respectively. Using chessboard titration method, three critical reagents concentration of qPCR were optimized and described. After that, 130 of collected isolates from food samples were confirmed by the developed protocols. Lastly, 27 enriched broths culture-positive for Campylobacter spp. were subjected to direct qPCR. The earlier results indicated that the diluted DNA templates improved the amplification plot intensity (∆Rn ˃ 225.000) and the amendment of the amount of specific primers, probes, as well as the concentration of MgCl2 on the amplification curves affected positively the fluorescence signal for both C. jejuni and C. coli established protocols. The confirmation of 130 of C. jejuni and C. coli isolated from broiler chickens, turkeys, cattle, and handling surface swabs by qPCR optimized protocols demonstrated similar outcomes as phenotypic tests. From 27 broiler chickens’ samples, C. coli were the most isolated strain from culture method, biochemically confirmed, compared to C. jejuni. In contrast, the application of qPCR directly on enriched broth revealed higher percentage (74.07%) of co-existence of C. coli and C. jejuni from the same analyzed samples. The present study developed and validated reliable, rapid, robust, and specific qPCR method for screening, quantifying, and confirming Campylobacter in food safety monitoring.

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Prevalence of the phenicol resistance gene fexA in Campylobacter isolated from the poultry supply chain

Tang

Zheng

Lin

et al. 2022

International Journal of Food Microbiology

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Prevalence and antimicrobial profiling of Campylobacter spp. isolated from meats, animal, and human feces in Northern of Morocco

Cited by 12 publications

References 45 publications

Isolation, identification and antibiotic resistance profile of thermophilic Campylobacter species from Bovine, Knives and personnel at Jimma Town Abattoir, Ethiopia

Isolation, identification and antibiotic resistance profile of thermophilic Campylobacter species from Bovine, Knives and personnel at Jimma Town Abattoir, Ethiopia

Development and Evaluation of Real Time PCR for Detection and Identification of C. jejuni and C. coli from Human Food

Prevalence of the phenicol resistance gene fexA in Campylobacter isolated from the poultry supply chain

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