Prevalence and Antimicrobial Resistance Assessment of Subclinical Mastitis in Milk Samples From Selected Dairy Farms

Objective: The present research work was undertaken with the objectives to investigate the prevalence and molecular detection of the causal agents of subclinical mastitis (SCM) in cows at milk shed areas in Sirajganj and Pabna districts, Bangladesh. Materials and methods: A total of 300 milk samples were randomly collected from Baghabari milk shed areas of Sirajganj and Pabna districts. The milk samples were subjected for California Mastitis Test (CMT) for identifying SCM. Total 81 positive samples were then used for the isolation and identification of associated bacteria and fungi using conventional microbiological examination and biochemical tests, followed by confirmation by polymerase chain reaction (PCR) using specific primers. Besides, universal primers were used for amplification and sequencing of PCR products where specific primers were not used. Results: The overall prevalence of SCM was 51% (n=153/300). Based on bacteriological examination and biochemical tests, several bacteria were identified in this study; the orgnaisms included Staphylococcus sp. KEYWORDSAcinetobacter sp.; Cow; rfbO157; PCR; Proteus sp.; Salmonella sp. AFFILIATIONS

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“…Acinetobacter sp. was isolated from mastitis in our study, as supported by Marimuthu et al (2014). Similary, Proteus sp.…”

Section: Resultssupporting

confidence: 60%

Prevalence and molecular detection of the causal agents of sub-clinical mastitis in dairy cows in Sirajganj and Pabna districts, Bangladesh

et al. 2017

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“…In contrast to our study, Sumathi et al [14], Dubal et al [15], Ranjan et al [16], Harini and Sumathi [17], Kurjogi and Kaliwal [18], Abd-Elrahman [19] and Jeykumar et al [20] observed E. coli being second in prevalence following staphylococci. However, Marimuthu et al [21] found 21% of organisms isolated to be Bacillus spp. which was totally absent in our study and other studies mentioned earlier.…”

Section: Prevalence Of Different Microorganismsmentioning

confidence: 99%

Pathogens isolated from clinical mastitis in Murrah buffaloes and their antibiogram

Charaya¹,

Sharma²,

Kumar³

et al. 2014

Vet World

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Aim: To determine the etiology of clinical mastitis in Murrah buffaloes and to develop an antibiogram of organisms isolated. Materials and Methods:A total of 564 quarter milk samples of 144 buffaloes suffering from clinical mastitis received in Veterinary College Central Laboratory were inoculated on blood agar, MacConkey's lactose agar and sabouraud dextrose agar. Bacteria isolated were characterized to the species level and subjected to in-vitro antimicrobial sensitivity testing.Results: Out of 564 quarters examined for mastitis, 320 (56.73%) quarters were found culturally positive showing isolation of Staphylococcus aureus 140 (38.04%), Streptococcus dysgalactiae 112 (30.43%), Streptococcus agalactiae 13 (3.53%), Escherichia coli 74 (20.10%) and Corynebacterium pyogenes 29 (7.88%). On carrying antibiogram staphylococci and streptococci revealed high sensitivity towards chloramphenicol, gentamicin, amikacin and enrofloxacin. Streptococci showed high sensitivity towards ceftriaxone and cefaperazone also. E. coli was found highly sensitive to chloramphenicol and gentamicin. C. pyogenes was sensitive to the majority of antibiotics. Conclusion:S. aureus was the most predominant bacteria isolated from mastitis cases and irrespective of the isolates chloramphenicol was found to be most sensitive when tested in-vitro.

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“…The main treatment of mastitis is commonly administered by intramammary infusion of an ointment or intramuscular or intravenous injection of antibiotics, such as streptomycin, ampicillin, cloxacillin, penicillin, and tetracycline [8]. However, the treatment is anticipated to become problematic in the near future owing to the rapid increase in antibiotic-resistant pathogens [20, 21]. Therefore, seeking for treatments alternative to antibiotic therapy is required.…”

Section: Introductionmentioning

confidence: 99%

Effective Treatment of Bovine Mastitis with Intramammary Infusion of Angelica dahurica and Rheum officinale Extracts

Yang

et al. 2019

Evidence-Based Complementary and Alternative Medicine

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Mastitis in dairy cattle is a highly prevalent infectious disease, causing considerable economic loss worldwide. In this study, we used Angelica dahurica and Rheum officinale extracts (designated as Yi-Xiong-Tang, YXT) for mastitis treatment. California mastitis test (CMT) was performed and 67 mastitis udder quarters were identified among 179 lactating dairy cows. These 67 mastitis udder quarters were subjected to treatments by intramammary infusion of YXT twice a day for three consecutive days. The mastitis indicators including clots, lactate dehydrogenase (LDH), TNF-α, IL-6, IL-8, and total viable count of bacteria (TVC) in milk were examined before and after the YXT treatment to evaluate its effectiveness. Levels of mastitis indicators from mastitis udder quarters were elevated. After YXT treatment, normal levels of these indicators were restored: TVC, 2.10 × 104 – 9.20 × 106 CFU/mL; clots, 6.56 ± 0.43 mg/mL; LDH, 181.0 ± 18.55 U/L; TNF-α, 0.02 ± 0.02 ng/mL; IL-6, 41.4 ± 11.46 pg/mL; and IL-8, 1.85 ± 0.60 pg/mL. Compared with the antibiotic therapy, YXT treatment has a shorter treatment course and might have lower probability for the causative agents to develop drug resistance because YXT is in fact a cocktail containing multiple active ingredients.

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Prevalence and Antimicrobial Resistance Assessment of Subclinical Mastitis in Milk Samples From Selected Dairy Farms

Cited by 14 publications

References 20 publications

Prevalence and molecular detection of the causal agents of sub-clinical mastitis in dairy cows in Sirajganj and Pabna districts, Bangladesh

Prevalence and molecular detection of the causal agents of sub-clinical mastitis in dairy cows in Sirajganj and Pabna districts, Bangladesh

Pathogens isolated from clinical mastitis in Murrah buffaloes and their antibiogram

Effective Treatment of Bovine Mastitis with Intramammary Infusion of Angelica dahurica and Rheum officinale Extracts

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