Prevalence and molecular detection of the causal agents of sub-clinical mastitis in dairy cows in Sirajganj and Pabna districts, Bangladesh

Kabir, Md. Humayun; Ershaduzzaman, Md.; Giasuddin, M; Nazir, K. H. M. Nazmul Hussain; Mahmud, Md. Muket; Islam, Md. Rafiqul; Islam, Mohammed Sirajul; Karim, Md. Rezaul; Yousuf, Md. Abu; Rahman, Seikh Masudur; Ali, Md. Yousuf

doi:10.5455/javar.2017.d238

Cited by 7 publications

(5 citation statements)

References 22 publications

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“…According to the present study, the prevalence of subclinical mastitis (SCM) was higher and this finding was sim-ilar in comparison to some previous findings. The current result was supported by Rahman et al (2014); Tripura et al (2014); Kabir et al (2017), who reported 50%-60% incidences of SCM in dairy cows on bacteriological examination. The finding of the present study was also supported by Kader et al (2002), who reported a 46.6 % prevalence of SCM in Bangladesh but the results disagreed with the findings of Rahman et al (2010).…”

Section: Discussionsupporting

confidence: 78%

Association of Udder Lesions with Subclinical Mastitis in Dairy Cows of Chattogram, Bangladesh

Hasan¹,

Kober²,

Rana³

et al. 2021

AAVS

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Mastitis is an economically important production disease in Bangladesh. A cross-sectional study was conducted to determine the association of udder lesions with Subclinical Mastitis (SCM) in dairy cows of Chattogram Metropolitan Area (CMA) and Patiya Upazila of Chattogram district during the period from December 2017 to May 2018. For this, 264 milk samples and 155 swab samples were collected from 50 different dairy farms available in CMA and Patiya Upazila. A pretested questionnaire was implemented during sample collection for identifying the associated risk factors of udder lesions and SCM, and to identify the association of udder lesions with SCM. Clinical inspection, palpation and observation were the main tools for determining the udder lesions whereas California Mastitis Tests (CMT) were used for the diagnosis of SCM. Milk samples from CMT positive cows and swab samples from cows' udder surface with lesions were collected for laboratory analysis. Laboratory analysis including bacterial culture and biochemical tests were done to identify the causal agents of SCM and udder lesions. The overall prevalence of SCM and udder lesions were 56.8% and 32.1%, respectively. Most of the farms under study were small scale (94%) except a few were medium-scale (6%). Very few farms used quarantine and isolation shed (6%) for the disease affected cows. Among the risk factors identified; type of farm, floor type, quarantine facility, isolation shed used, adequate drainage facility were statistically significant (P ≤ 0.05) to causing both SCM and udder lesions. Predominant causal agents isolated were Staphylococcus sp 186 (70.4%), Streptococcus sp 146 (55.3%), Bacillus sp 62 (23.5%) and E. coli 20 (7.6%) from collected CMT positive milk samples. Bacterial cultures of swab samples of udder lesions revealed Staphylococcus sp 120 (77.4%), Streptococcus sp 112 (72.2%), E. coli 110 (71.0%), and Bacillus sp 47 (30.3%). Moreover, udder lesions were associated with the occurrence of SCM in dairy cows. Therefore, udder damages should be managed carefully to minimise SCM prevalence in dairy cows.

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Section: Discussionsupporting

confidence: 78%

Association of Udder Lesions with Subclinical Mastitis in Dairy Cows of Chattogram, Bangladesh

Hasan¹,

Kober²,

Rana³

et al. 2021

AAVS

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“…Although the provision of SCC is readily available to most dairy farms in developed countries on a monthly basis through the dairy herd improvement (DHI) program, this practice yet not accustomed in Bangladesh. Because of the high prevalence (30-72%) of SCM and abundance of the mastitis pathogens [14][15][16][17][18][19], the provision of SCCs for routine SCM screening in dairy herds will be of great benefit in reducing the occurrence of SCM and ensuring milk quality as well.…”

Section: Introductionmentioning

confidence: 99%

Relationship between somatic cell counts and subclinical mastitis in lactating dairy cows

et al. 2020

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Background and Aim: Subclinical mastitis (SCM) is one of the most prevalent diseases of dairy cows, and somatic cell count (SCC) is widely used to determine SCM and milk quality. However, setting the threshold of SCC is very important. This study aimed to determine the cow-level threshold of SCC to differentiate milk of SCM affected cows from normal milk in Bangladesh. Materials and Methods: Sensitivity (Se) and specificity (Sp) along with other characteristics of different thresholds of SCC were determined considering the bacterial culture as the gold standard test. Three definitions of intramammary infection (IMI) were set based on the group of pathogens involved. Five categories of SCC thresholds were considered for analysis. Results: Se and Sp of thresholds of SCC greatly varied as definitions of IMI changed. Irrespective of SCC thresholds, Se increased when isolation of major pathogens included in IMI definitions. Se decreased when SCC thresholds increased (from 100 to 300 × 103 cells/mL) for all IMI definitions and ranged from 60.6% to 20.3%. The highest Se was found at low SCC threshold (100 × 103 cells/mL), which resulted in less false-negative outcome. On the other hand, Sp increased with the increment of SCC thresholds giving rise to a less false-positive outcome. Conclusion: The cow-level SCC threshold of 100 × 103 cells/mL was found appropriate to identify subclinical IMI of dairy cows.

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“…In the present study, specific enriched media were used for the isolation and identification of Salmonella spp. which was also used by a number of researchers such as (Kabir et al, 2017). The morphology of the isolated Salmonella spp.…”

Section: Resultsmentioning

confidence: 99%

Microbiological Quality Assessment of Marketed Broiler Meat in Different Markets of Dhaka City

Sultana

Mukta

Biswas

et al. 2020

Res. Agric. Livest. Fish.

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The present research work was undertaken to assess the bacterial quality and to know the prevalence of zoonotic bacteria from broiler meat samples sold in Krishi market, Bihari camp market, Agargaon market, Taltola market and SAU (Sher-e-Bangla Agricultural University) mini bazar, Dhaka, Bangladesh from January to May 2018. After processing of samples primary culture was done in nutrient broth and nutrient agar media then pure culture was obtained from different selective media. Total Viable Count (TVC), Total Coliform Count (TCC) and Total Salmonella Count (TSC) in broiler meat of different broiler markets were determined. Mean of TVC, TCC and TSC for the Krishi market, Agargoan market, Taltola market, Bihari camp market and SAU Mini market were 5.67, 4.32, 2.96 log10 CFU/g, 5.88, 4.64, 3.56 log10 CFU/g, 6.10, 4.68, 3.78 log10 CFU/g, 6.68,4.87, 3.84 log10 CFU/g and 5.84, 4.25, 3.13 log10 CFU/g respectively. The prevalence of Escherichia coli and Salmonella spp. Were 74% and 42% respectively. E. coli isolates were showed sensitive to Ciprofloxacin (91.6%), Gentamycin (87.5%), Azithromycin (66.66%), and Tetracycline (58%) and resistant to Penicillin (79.16%) then Amoxycillin (75%), Streptomycin (75%) and Ampicillin (58.3%). Highest resistant pattern was showed by Tetracycline (58%), Streptomycin (72.72%), Penicillin (72.72%) and Amoxycillin (63.63%). Highest resistant pattern showed by Amoxycillin (71.42%) and Penicillin (71.42%). This study revealed that broiler meat sold at some local markets of Dhaka city were contaminated with multiple species of multidrug resistant bacteria which may risk for human health. Res. Agric., Livest. Fish.7(2): 261-266, August 2020

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Prevalence and molecular detection of the causal agents of sub-clinical mastitis in dairy cows in Sirajganj and Pabna districts, Bangladesh

Cited by 7 publications

References 22 publications

Association of Udder Lesions with Subclinical Mastitis in Dairy Cows of Chattogram, Bangladesh

Association of Udder Lesions with Subclinical Mastitis in Dairy Cows of Chattogram, Bangladesh

Relationship between somatic cell counts and subclinical mastitis in lactating dairy cows

Microbiological Quality Assessment of Marketed Broiler Meat in Different Markets of Dhaka City

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