Prevalence and Antimicrobial Resistance of Diarrheagenic Escherichia coli and Shigella Species Associated with Acute Diarrhea in Tehran, Iran

Abstract

A study was performed to determine the prevalence and antimicrobial resistance of Shigella species and diarrheagenic Escherichia coli isolates cultured from patients with acute diarrhea in Tehran, Iran. Between May 2003 and May 2005, 1120 diarrheal specimens were collected and assayed for bacterial enteropathogens by conventional and molecular methods. Etiological agents were isolated from 564 (50.3%) specimens, and included 305 (54%) E coli, 157 (27.8%) Shigella species, and 102 (18%) from other genera of bacteria. The predominant E coli was Shiga toxin-producing E coli (105 isolates [34.5%]) and the predominant Shigella serotype was Shigella sonnei (88 isolates [56.1%]). A high rate of antibiotic resistance was observed among E coli, with 40 of 53 (75.5%) Shiga toxin-producing E coli isolates resistant to amoxicillin and tetra-cycline, and eight (5.2%) E coli isolates resistant to more than six antibiotics. Most Shigella isolates were resistant to tetracycline (95%) and trimethoprim-sulfamethoxazole (91.7%), with greatest antibiotic resistance observed among S sonnei (53 of 88 [60.2%] isolates). Antibiotic resistance is widespread in diarrheagenic E coli and Shigella in children with acute diarrhea in Tehran, Iran; hence, updated strategies for appropriate use of antimicrobial agents in Iran are needed.

Evaluation of Prevalence, Homology and Immunogenicity of Dispersin among Enteroaggregative Escherichia coli Isolates from Iran

“…For example, one large prospective surveillance study in the USA has indicated that EAEC strains are the most common causative agents of diarrhea in all age groups [1] . Other studies have shown that EAEC strains are among the important diarrheagenic strains isolated from Iranian patients [14][15][16][17][18] . On the other hand, treatment of the EAEC infections has become increasingly difficult due to the emergence of resistant pathogens [2] .…”

Molecular Detection of blaTEM and blaSHF in Diarrhoegenic Escherichia coli Isolated from Egyptian Children

“…Many studies indicate that multidrug resistant E. coli are widespread among the DEC strains and occurrence of resistant DEC could be because of environmental conditions, including transmission of resistant isolates from adults to children, or from animals to humans [38]. The main factors for dissemination of resistance genes are mobilizable plasmids, self-transmissible plasmids and conjugative transposons [39].…”

“…The disease remains a health problem challenge especially in developing countries [1][2][3]. The causative pathogens include a wide variety of microorganisms like bacteria, viruses and parasites.…”

Shigella Species Isolated from Stool Specimens between 2002 and 2011 Years and their Antimicrobial Resistance Profiles

“…Altun ve Gür (2) çalışmamıza paralel olarak % 74.2 genel direnç oranı bulurlarken, S.sonnei için daha yüksek direnç oranı bildirmişlerdir. Ülkemizde ve yurt dışında yapı-lan bir çok çalışmada oldukça yüksek direnç oranları (% 55-92) verilmektedir (1,3,(11)(12)(13)(14)17,21) . Çalışmamızda son beş yılda elde edilen direnç verilerinin ilk beş yıla oranla yükseldiği görül-mektedir (Tablo 3).…”