Prevalence and Antimicrobial Resistance Pattern of Extended Spectrum Beta Lactamase Producing Gram Negative Bacterial Isolates Obtained From Various Clinical Samples of Indoor Patients Admitted in a Tertiary Care Hospital

Chopra, Akanksha; Singh, Kanwardeep; Sidhu, Stanley; Oberoi, Loveena; Kaur, Arpandeep

doi:10.21276/ijcmr.2019.6.3.17

IJCMR

2019

DOI: 10.21276/ijcmr.2019.6.3.17

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Prevalence and Antimicrobial Resistance Pattern of Extended Spectrum Beta Lactamase Producing Gram Negative Bacterial Isolates Obtained From Various Clinical Samples of Indoor Patients Admitted in a Tertiary Care Hospital

Akanksha Chopra¹,

Kanwardeep Singh²,

Stanley Sidhu³

et al.

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Cited by 2 publications

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Study Gene Expression of blaOXA-48 andblaVIM-1Genes CarbapenemsResistance in Klebsiella Pneumoniae isolated from urinary tract infections in Baghdad hospital's

Hafez Aboud,

M. Shami,

Jawad Kadhim

2023

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Klebsiella pneumoniae has emerged as one of the eight most essential causes of urinary tract infection (UTI), with increasing resistance to antibiotics, especially carbapenems. About one hundred-eight urine samples were collected from inpatients and outpatients who attended. They were collected at three Baghdad hospitals: two from Karkh (AL-Yarmouk Hospital and Al-karamu Hospital) and one from Rusafa (AL-Baghdad Educational Hospital). A minimal inhibitory concentration (MIC) test was done using the Microtiter plate method and demonstrated different resistance levels against meropenem antibiotics. Gene expression of blaOXA-48 and blaVIM-1 genes was performed when treated with meropenem antibiotic using the Real-time PCR technique. The study showed that the highest value of gene expression in the blaOXA-48 gene was recorded for the resistant group was (1.8705), and the lowest value of gene expression in the OXA-48 gene before treated with the meropenem group was (1.0312). The gene expression value in the non-treated group was (0.97) control. In contrast, A slight increase in gene expression value for the blaVIM-1 gene was recorded for the meropenem-treated group (1.8705), and the sensitive group had a gene expression value of (1.00). The lowest value was in the gene expression of the meropenem-treated group (1.0312), as well as an increase in the value of the expression. The genotype of the blaVIM-1gene for the meropenem-treated group (1.828) is based on the 2-ΔCt method for both genes. When using the 2-ΔΔCt method, gene expression differed slightly in both genes. When relying on the 2- ΔΔCt method, both genes' gene expression was slightly different. It was concluded that the coexistence of blaVIM-1 and blaOXA-48 genes in four strains of K. pneumoniae indicated widespread VIM-1 and OXA-48 in Baghdad, Iraq. Keywords: Klebsiella Pneumonia, blaOXA-48,and blaVIM-1, UTI infection

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Study Gene Expression of blaOXA-48 andblaVIM-1Genes CarbapenemsResistance in Klebsiella Pneumoniae isolated from urinary tract infections in Baghdad hospital's

Hafez Aboud,

M. Shami,

Jawad Kadhim

2023

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Phenotypic Identification of Extended Spectrum Beta Lactamase and Metallo Beta Lactamase Production Among Isolates of Enterobacteriaceae in a Tertiary Care Hospital, Udaipur, Rajasthan

Sharma

Jain

Tofiq

2022

IJAR

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Antimicrobial resistance is a growing threat worldwide. Increasing resistance to third generation cephalosporins has become a cause for more concern among Enterobacteriaceae. The prevalence of extended spectrum -lactamases (ESBLs) and metallobetalactamases (MBL) among members of Enterobacteriaceae constitutes a serious threat to current situation of -lactam therapy leading to treatment failure. 100 Enterobacteriaceae isolated from various clinical samples were included in the study. ESBL was detected by phenotypic conrmatory disc diffusion test (PCDDT) using ceftazidime alone and in combination with clavulanic acid. MBLdetection was done by Imipenem EDTA combined disc diffusion test. Out of 100 Enterobacteriaceae isolates, 47(47%) were ESBL producers and 29 (29%) were MBLproducers. None of the isolates showed the coexistence of ESBLand MBLin the same isolate. ESBLand MBLproduction was observed in E.coli, Klebsiella spp., Acinetobacter spp. and Citrobacter spp. isolated from various clinical samples. The study underlines problem of ESBL and MBL mediated resistance, which has created a therapeutic challenge for the clinicians and microbiologists. Simple disk method can be routinely employed to detect these common resistance mechanisms which will reduce the mortality and also spread of such resistant strains.

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Prevalence and Antimicrobial Resistance Pattern of Extended Spectrum Beta Lactamase Producing Gram Negative Bacterial Isolates Obtained From Various Clinical Samples of Indoor Patients Admitted in a Tertiary Care Hospital

Cited by 2 publications

References 5 publications

Study Gene Expression of blaOXA-48 andblaVIM-1Genes CarbapenemsResistance in Klebsiella Pneumoniae isolated from urinary tract infections in Baghdad hospital's

Study Gene Expression of blaOXA-48 andblaVIM-1Genes CarbapenemsResistance in Klebsiella Pneumoniae isolated from urinary tract infections in Baghdad hospital's

Phenotypic Identification of Extended Spectrum Beta Lactamase and Metallo Beta Lactamase Production Among Isolates of Enterobacteriaceae in a Tertiary Care Hospital, Udaipur, Rajasthan

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