Prevalence and Antimicrobial Susceptibility Patterns of Bacteria from Milkmen and Cows with Clinical Mastitis in and around Kampala, Uganda

Kateete, David Patrick; Kabugo, Usuf; Baluku, Hannington; Nyakarahuka, Luke; Kyobe, Samuel; Okee, Moses; Najjuka, Christine F.; Joloba, Moses

doi:10.1371/journal.pone.0063413

Cited by 104 publications

(118 citation statements)

References 31 publications

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“…This is in contrast to the report by Kateete et al, (2013) that isolated Lactobacillus species from only 9% of milk samples analysed. The detection of Lactobacillus species in the studied milk products is indicative of its use as starter cultures in the production /fermentation of milk and milk products (Medicinal Veterinary Animal Health, 2016).…”

Section: Discussioncontrasting

confidence: 99%

Assessment of microbiological and residual antibiotics status in milk sold in Abeokuta, Ogun State. Nigeria

Oluwafemi

Oluwabamiwo

Oluwatosin

et al. 2018

Turkish JAF Sci.Tech.

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This study investigated the prevalence and concentration of antibiotic residues in twenty brands of milk. Determination and quantification of antibiotic residues was done using High Performance Liquid Chromatography. Aerobic plate count was carried out and the associated bacteria isolated. Identification of bacteria was done using standard microbiological methods. Molecular characterization and identification of bacterial isolates was done using 16S rRNA gene sequencing method. Antibiotic sensitivity was carried out on bacterial isolates using disc diffusion method. Plasmid profile of drug resistant isolates was done using alkaline analysis method. Comparison of means was done using Analysis of Variance. Antibiotic residues were detected in 10 (50%) of the 20 milk brands analyzed. The residual levels of tetracycline, oxytetracycline and chlortetracycline ranged between 5ng/kg and 1569ng/kg while none of the samples had doxycycline residues. The prevalence of tetracycline residues in evaporated milk brands and powdered milk samples were 100 and 23 % respectively. The aerobic plate counts ranged from 2.5 × 10 2 to 6.5 × 10 2 CFU∕mL for evaporated milk and 2.5 × 10 1 to 6.0 × 10 1 CFU∕mL for powdered milk. A total of ten bacterial species were isolated and identified. Susceptibility result showed that 95% of the isolates were sensitive to erythromycin, cefuroxime and gentamycin; 12.5% were resistant to tetracycline and ampicillin, 10% were resistant to ofloxacin and 7.5% to doxycycline and penicillin. Five of the bacterial isolates were resistant to more than one class of antibiotics. Resistant isolates subjected to plasmid profiling had detectable plasmids with estimated sizes between 120bp and 1000bp. The obtained results provide evidence that the presence of antibiotic residues in evaporated and powdered milk is an indication that the public is exposed to the harmful effects of the residues.

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Section: Discussioncontrasting

confidence: 99%

Assessment of microbiological and residual antibiotics status in milk sold in Abeokuta, Ogun State. Nigeria

Oluwafemi

Oluwabamiwo

Oluwatosin

et al. 2018

Turkish JAF Sci.Tech.

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“…The farm result was slightly lower than 15.5% (51/328), [31] in around Addis Ababa; 17.2%, [32] in Egypt and 19.5%, [33] who isolated S. aureus strains from human and animal sources. Of the farm sample 5/42 (11.9%), 1/9 (11.1%), 0 (0.0%), 3/9 (33.3%), 1/9 (11.1%), and 3/9 (33.3%); udder milk, tank milk, polled bucket swab, tank swab, polled hand and nasal swab, respectively, were positive for S. aureus The findings of the present study revealed a lower proportional rate than 75% in 220 bovine bulk milk reported in [34], 68% (15/22) in [5], 61.3% (49/80) in [35], and 40% (32/81) in [36], but higher than [37] which was 8% (8/100) in udder milk and 10% (10/100) in tank milk. Also the present result shows the proportion of S. aureus in polled farm nasal swab 3/9 (33.3%) was higher than [38], 4/31(13%) and [33] who reported 20% from nasal swabs of diseased human.…”

Section: Citationmentioning

confidence: 43%

Staphylococcus: Isolation, Identification and Antimicrobial Resistance in Dairy Cattle Farms, Municipal Abattoir and Personnel in and Around Asella, Ethiopia

Abunna¹,

Abriham²,

Gizaw³

et al. 2016

J Vet Sci Technol

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A cross sectional study was conducted between February, 2014 and April, 2014 to isolate and identify Staphylococcus from dairy cattle farms and municipal abattoir; and to evaluate antimicrobial sensitivity for isolates in and around Asella, Ethiopia. An over all of 181 samples were collected and processed from nine dairy cattle farms (87) and seven municipal abattoir visits (94). Accordingly, 42 (23.2%) udder milk, 9 (5.0%) tank milk, 9 (5.0%) polled bucket swab, 9 (5.0%) tank swab, 9 (5.0%) polled hand swab, 9 (5.0%) polled nasal swab, from dairy cattle farms; and 66 (36.5%) meat swab, 7 (3.9%) polled knife swab, 7 (3.9%) polled slaughter line swab, 7 (3.9%) polled hand swab and 7 (3.9%) polled nasal swab from municipal abattoir visits were collected. The result showed the overall proportion of Staphylococcus was 89 (49.2%). Staphylococcal species were more predominant in abattoir 50/94 (53.2%) than farms 39/87 (44.8%), but there was no significant difference between them because p>0.05 at 95% confidence interval. Also high proportion of Staphylococcus was isolated from polled farm nasal swab 8/9 (88.9%), but this difference between sample type and the presence of Staphylococcus is not significant, because p-value (0.303) is greater than 0.05 at 0.05 level. Up on isolation and identification 35 (19.3%), 6 (3.3%), 24 (13.3%), 24 (13.3%) were S. aureus, S. intermedius, S. hyicus and Coagulase Negative Staphylococci (CNS), respectively. From total positive samples, 55 isolates were tested for antimicrobial susceptibility to different 15 antimicrobial discs. The comparative efficacies of antimicrobials used indicates Gentamycin, Kanamycin, Chloramphenicol, Ciprofloxacillin, and Sulphamethoxazole trimethoprim, were the most effective antibiotics where by 94.5%, 89.1%, 81.8%, 81.8%, and 81.8% respectively. Good hygienic practices should be followed both in dairy cattle farms and municipal abattoir including working personnel and equipment's used; and antimicrobials susceptibility test should be carried out at regular intervals to find out the development of resistance against the most commonly applied antibiotics.

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“…At present, little is known about the occurrence of molecular types and antimicrobial resistance (AMR) profile of S. aureus isolates from bovine subclinical mastitis in India. Changes in mastitis isolate profiles influenced by setting have been reported earlier (3), which again emphasizes the need for periodic evaluation of S. aureus in terms of virulence and AMR. Currently, penicillin, erythromycin, and tetracycline are frequently used for the treatment of mastitis.…”

Section: Introductionmentioning

confidence: 53%

Molecular types and antimicrobial resistance profile of Staphylococcus aureus isolated from dairy cows and farm environments

Gandhale¹,

Kolhe²,

Nalband³

et al. 2017

Turk J Vet Anim Sci

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Prevalence and Antimicrobial Susceptibility Patterns of Bacteria from Milkmen and Cows with Clinical Mastitis in and around Kampala, Uganda

Cited by 104 publications

References 31 publications

Assessment of microbiological and residual antibiotics status in milk sold in Abeokuta, Ogun State. Nigeria

Assessment of microbiological and residual antibiotics status in milk sold in Abeokuta, Ogun State. Nigeria

Staphylococcus: Isolation, Identification and Antimicrobial Resistance in Dairy Cattle Farms, Municipal Abattoir and Personnel in and Around Asella, Ethiopia

Molecular types and antimicrobial resistance profile of Staphylococcus aureus isolated from dairy cows and farm environments

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