Prevalence and characteristics of Shiga-toxin producing Escherichia coli (STEC) isolated from beef slaughterhouse

Parvej, Md. Shafiullah; Mamun, M. M.; Hassan, Jayedul; Mahmud, Md. Muket; Rahman, Mohammad Mizanur; Rahman, Md. Tanvir; Rahman, Bahanur; Nazir, K. H. M. Nazmul Hussain

doi:10.5455/javar.2018.e271

Cited by 5 publications

(3 citation statements)

References 31 publications

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“…NA resistance was most common, along with TE, AM, C, SXT, and CIP, while most of the strains were sensitive to CZ, AMC, CRO, and FOX. Similar findings of common resistance to NA, TE, AM, SXT, and CIP among E. coli isolates from avian origin and other food animals have been reported by many researchers from China (44), Egypt (45), France (46), Bangladesh (47), and Japan (19). Although phylogroups D and B2 were related to higher drug resistance patterns in previous reports (48), we did not find any B2 strains in poultry.…”

Section: Discussionsupporting

confidence: 89%

Host Range-Associated Clustering Based on Multilocus Variable-Number Tandem-Repeat Analysis, Phylotypes, and Virulence Genes of Atypical Enteropathogenic Escherichia coli Strains

Parvej

Nakamura

Alam

et al. 2019

Appl Environ Microbiol

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Atypical enteropathogenicEscherichia coli(aEPEC) strains (36 Japanese and 50 Bangladeshi) obtained from 649 poultry fecal samples were analyzed by molecular epidemiological methods. Clermont’s phylogenetic typing showed that group A was more prevalent (58%, 50/86) than B1 (31%, 27/86). Intimin type β1, which is prevalent among human diarrheal patients, was predominant in both phylogroups B1 (81%, 22/27) and A (70%, 35/50). However, about 95% of B1-β1 strains belonged to virulence group I, and 77% of them were Japanese strains, while 17% (6/35) of A-β1 strains did. Multilocus variable-number tandem-repeat analysis (MLVA) distributed the strains into 52 distinct profiles, with Simpson’s index of diversity (D) at 73%. When the data were combined with those of 142 previous strains from different sources, the minimum spanning tree formed five zones for porcine strains, poultry strains (excluding B1-β1), strains from healthy humans, bovine and human patient strains, and the B1-β1 poultry strains. Antimicrobial resistance to nalidixic acid was most common (74%) among the isolates. Sixty-eight percent of them demonstrated resistance to ≥3 antimicrobial agents, and most of them (91%) were from Bangladesh. The strains were assigned into two groups by hierarchical clustering. Correlation matrix analysis revealed that the virulence genes were negatively associated with antimicrobial resistance. The present study suggested that poultry, particularly Japanese poultry, could be another reservoir of aEPEC (phylogroup B1, virulence group I, and intimin type β1); however, poultry strains seem to be apart from patient strains that were closer to bovine strains. Bangladeshi aEPEC may be less virulent for humans but more resistant to antibiotics.IMPORTANCEAtypical enteropathogenicEscherichia coli(aEPEC) is a diarrheagenic type ofE. coli, as it possesses the intimin gene (eae) for attachment and effacement on epithelium. Since aEPEC is ubiquitous even in developed countries, we previously used molecular epidemiological methods to discriminate aEPEC as a human pathogen. The present study assessed poultry as another source of human diarrheagenic aEPEC. Poultry could be the source of aEPEC (phylogroup B1, virulence group I, and intimin type β1) found among patient strains in Japan. However, the minimum spanning tree (MST) suggested that the strains from Japanese poultry were far from Japanese patient strains compared with the distance between bovine and patient strains. Bangladeshi avian strains seemed to be less diarrheagenic but are hazardous as a source of drug resistance genes.

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Section: Discussionsupporting

confidence: 89%

Host Range-Associated Clustering Based on Multilocus Variable-Number Tandem-Repeat Analysis, Phylotypes, and Virulence Genes of Atypical Enteropathogenic Escherichia coli Strains

Parvej

Nakamura

Alam

et al. 2019

Appl Environ Microbiol

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“…Author(s) agree that this article remain permanently open access under the terms of the Creative Commons Attribution License 4.0 International License facultative anaerobe that does not generate spores (Parvej et al, 2018). The cells are generally rod-shaped, with a diameter ranging from 0.2 to 1 μm and a length of around two μm.…”

Section: Introductionmentioning

confidence: 93%

“…VTEC isolates are commonly present in food. Predictions are made of the possible increase in problems associated with these emerging pathogens (Younus et al, 2020;Parvej et al, 2018). As time passed, the food habits of ordinary people have changed rapidly in Bangladesh.…”

Section: Introductionmentioning

confidence: 99%

Isolation, identification and antibiogram of verotoxin producing Escherichia coli from raw salad vegetables at Jashore, Bangladesh

Meghla¹,

Mridha²,

Rana³

et al. 2021

Afr. J. Microbiol. Res.

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Verotoxin-producing E. coli are getting an emergence for human health and day by day, it is also becoming more alarming in the fact that, they are acquiring multidrug-resistant profiles. This study aimed at isolation and identification of verotoxigenic E. coli followed by the antibiogram of the species from salad vegetables at Jashore district in Bangladesh. A total of 119 raw vegetable salad samples were collected from different areas of the Jashore district. The collected samples were initially enriched in nutrient broth and then used for streak plating on MacConkey and followed by Eosin Methylene Blue (EMB) agar media. Escherichia coli species were isolated and identified following observation of the cultural, microscopic, and biochemical characteristics and further analyzed to determine the presence of verotoxin (VT) producing genes through polymerase chain reaction (PCR) using specific primers (VT1, VT2, and Eae). A panel of antibiotics were tested following the disc diffusion method for determining the antibiotic profile. Total 55 (46.22%) E. coli was isolated phenotypically. 6 (10.9%) isolated E. coli showed positive by amplifying verotoxin-producing gene vt1, but none of the samples were positive vt2 and eaeA gene. Antibiogram for 55 E. coli isolates showed the highest resistance to Erythromycin (83.64%), Streptomycin (78.18%), Ceftriaxone (70.91%). On the other hand, the most heightened susceptibility was observed for Nalidixic acid (76.36%). Moreover, six vt1 positive isolates showed a multidrug-resistant profile. Thus, the presence of toxigenic genes in the isolates suggests the proper maintenance and regular monitoring of raw salad items should be increase to ensure healthy life in this region.

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Escherichia coli serogroups in slaughterhouses: Antibiotic susceptibility and molecular typing of isolates

Barel

Hızlısoy

Güngör

et al. 2022

International Journal of Food Microbiology

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Prevalence and characteristics of Shiga-toxin producing Escherichia coli (STEC) isolated from beef slaughterhouse

Cited by 5 publications

References 31 publications

Host Range-Associated Clustering Based on Multilocus Variable-Number Tandem-Repeat Analysis, Phylotypes, and Virulence Genes of Atypical Enteropathogenic Escherichia coli Strains

Host Range-Associated Clustering Based on Multilocus Variable-Number Tandem-Repeat Analysis, Phylotypes, and Virulence Genes of Atypical Enteropathogenic Escherichia coli Strains

Isolation, identification and antibiogram of verotoxin producing Escherichia coli from raw salad vegetables at Jashore, Bangladesh

Escherichia coli serogroups in slaughterhouses: Antibiotic susceptibility and molecular typing of isolates

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