Prevalence and Characterization of Extended-Spectrum Beta-lactamases-Producing<i>Salmonella enterica</i>Isolates in Saragossa, Spain (2001–2008)

Gándara, María Pardos de la; Seral, Cristina; García, Javier Castillo; Calvo, Carmen Rubio; Weill, François‐Xavier

doi:10.1089/mdr.2010.0105

Cited by 17 publications

(12 citation statements)

References 35 publications

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“…In addition, IncI1-I␥-type conjugative plasmids with sizes of ϳ75 kb (1 plasmid) and ϳ100 kb (2 plasmids) were also detectable in transconjugants. Studies from different regions of the world also reported the presence of IncI1 plasmids carrying bla CTX-M-14 and other bla CTX-M variants in Salmonella (22,25,26). Interestingly, the size of some of these plasmids (ϳ90 kb) was similar to the size of plasmids recoverable from the S. Enteritidis strains tested in this study.…”

Section: Antimicrobialsupporting

confidence: 76%

IncI1 Plasmids Carrying Various bla _CTX-M Genes Contribute to Ceftriaxone Resistance in Salmonella enterica Serovar Enteritidis in China

Wong

Kan

Chan

et al. 2016

Antimicrob Agents Chemother

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cResistance to extended-spectrum ␤-lactams in Salmonella, in particular, in serotypes such as Salmonella enterica serovar Enteritidis that are frequently associated with clinical infections, is a serious public health concern. In this study, phenotypic characterization of 433 clinical S. Enteritidis strains obtained from a nationwide collection of the Chinese Center for Disease Control and Prevention during the period from 2005 to 2010 depicted a trend of increasing resistance to ceftriaxone from 2008 onwards. Seventeen (4%) of the strains were found to be resistant to ceftriaxone, 7% were found to be resistant to ciprofloxacin, and 0.7% were found to be resistant to both ciprofloxacin and ceftriaxone. Most of the ceftriaxone-resistant S. Enteritidis strains (15/17) were genetically unrelated and originated from Henan Province. The complete sequence of an IncI1 plasmid, pSE115, which belonged to a novel sequence type, was obtained. This 87,255-bp IncI1 plasmid was found to harbor a bla CTX-M-14 gene in a novel multidrug resistance region (MRR) within the tra locus. Although the majority of strains were also found to contain conjugative IncI1 plasmids with a size similar to that of pSE115 (ϳ90 kb) and harbor a variety of bla CTX-M group 1 and group 9 elements, the novel MRR site at the tra locus in pSE115 was not detectable in the other IncI1 plasmids. The findings from this study show that cephalosporin resistance in S. Enteritidis strains collected in China was mainly due to the dissemination of IncI1 plasmids carrying bla CTX-M , resembling the situation in which IncI1 plasmids serve as major vectors of bla CTX-M variants in other members of the Enterobacteriaceae. Foodborne salmonellosis is a serious public health problem worldwide and the leading cause of foodborne illnesses in many countries, including the United States and China. Salmonella enterica subsp. enterica serovar Enteritidis is the most common serotype that causes human infections in China (1). In the United States, S. Enteritidis has become the most clinically prevalent serotype since 2008, accounting for ϳ17% of all salmonellosis cases (2). In other countries, the prevalence of S. Enteritidis is even higher, reaching Ͼ50% in European countries and Hong Kong (1, 3-5). Most importantly, S. Enteritidis is commonly associated with outbreaks of foodborne illness and is sometimes associated with invasive Salmonella infections worldwide (6). Although antimicrobials are usually not required for the treatment of salmonellosis due to the self-limiting nature of this disease, they can be lifesaving in patients with invasive infections, which normally occur in children, the elderly, and immunocompromised patients (7).Ceftriaxone and ciprofloxacin are the key drugs of choice for the treatment of invasive Salmonella infections in humans (8).Resistance to ceftriaxone or other extended-spectrum ␤-lactams is usually due to the production of extended-spectrum ␤-lactamases (ESBLs), among which the CTX-M-type ESBLs are largely responsible for cephalosporin resista...

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Section: Antimicrobialsupporting

confidence: 76%

IncI1 Plasmids Carrying Various bla _CTX-M Genes Contribute to Ceftriaxone Resistance in Salmonella enterica Serovar Enteritidis in China

Wong

Kan

Chan

et al. 2016

Antimicrob Agents Chemother

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“…Various studies previously reported the presence of IncI1 plasmids that carried the bla CTX-M-14 element and other bla CTX-M variants in Salmonella spp. (9)(10)(11). In addition, other plasmid types, such as IncF, FIIA, F1B, HI2, Y, and B/O, have also been reported to be associated with bla CTX-M-14 and other bla CTX-M elements in Salmonella enterica serotype Typhimurium (12).…”

mentioning

confidence: 99%

Dissemination of IncI2 Plasmids That Harbor the bla _CTX-M Element among Clinical Salmonella Isolates

Wong

Liu

Yan

et al. 2015

Antimicrob Agents Chemother

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cThe extended-spectrum-␤-lactamase (ESBL) determinant CTX-M-55 is increasingly prevalent in Escherichia coli but remains extremely rare in Salmonella. This study reports the isolation of a plasmid harboring the bla CTX-M-55 element in a clinical Salmonella enterica serotype Typhimurium strain resistant to multiple antibiotics. This plasmid is genetically identical to several known IncI2-type elements harbored by E. coli strains recovered from animals. This finding indicates that IncI2 plasmids harboring the bla CTX-M genes may undergo cross-species migration among potential bacterial pathogens, with E. coli as the major source of such elements. Salmonellosis is a serious public health problem worldwide (1-3). Ceftriaxone and ciprofloxacin are the choice of treatment for invasive Salmonella infections (4). Resistance to ceftriaxone or other extended-spectrum ␤-lactams (ESBL) is usually due to the CTX-M group ESBLs in Salmonella spp., with CTX-M-9, CTX-M-14, and CTX-M-15 the most commonly reported in China and several other regions of the world (5-7). Genes encoding CTX-Mtype ESBLs are usually located in transmissible plasmids, which tend to disseminate among members of the family of Enterobacteriaceae (8). Various studies previously reported the presence of IncI1 plasmids that carried the bla CTX-M-14 element and other bla CTX-M variants in Salmonella spp. (9-11). In addition, other plasmid types, such as IncF, FIIA, F1B, HI2, Y, and B/O, have also been reported to be associated with bla CTX-M-14 and other bla CTX-M elements in Salmonella enterica serotype Typhimurium (12).In addition to CTX-M-14 and CTX-M-15, CTX-M-55 was increasingly reported in various countries in recent years, particularly among Escherichia coli isolates of zoonotic origin (13-17). However, it has not been found in Salmonella isolates. This element is frequently associated with the transposase ISEcp1 and is increasingly found in the IncI2 plasmids (18). Recently, several IncI2 plasmids, which harbored the bla CTX-M-55 element, were sequenced (19,20). On the basis of the genetic similarity between elements recovered from different bacterial species and a major discrepancy in the prevalence rate of different bla CTX-M elements in E. coli and Salmonella isolates, we speculate that bla CTX-M -borne plasmids might originate from E. coli and possess the ability to disseminate to Salmonella and other bacterial species.In this study, we identified a bla CTX-M-55 -positive Salmonella Typhimurium strain named STH21 that was isolated from a stool specimen from a patient in 2008 in Henan Province, People's Republic of China. Antimicrobial susceptibility tests performed according to the CLSI guideline revealed that STH21 was resistant to various antimicrobial drugs, including ampicillin, ceftriaxone, cefotaxime, amikacin, ciprofloxacin, and azithromycin (Table 1) (21). A bla CTX-M-55 -bearing plasmid recovered from this strain and the corresponding cefotaxime resistance phenotype was transferred to E. coli J53 by conjugation (22). S1-pulsed-field gel ele...

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“…This is an indication that the isolates could be harboring cefotaximehydrolyzing β-lactamases (CTX-M types) or CMY-2 AmpC β-lactamase that could hydrolyze cephalosporins normally transferred through conjugative plasmids, transposons, or integrons [52,53]. This is supported by the fact that resistance to β-lactams in S. enterica is primarily caused by the production of acquired β-lactamases [54], with TEM-1, PSE-1, and OXA-1 having been described as the enzymes most frequently related to ampicillin and amoxicillin/clavulanate resistance. Thus, the resistance pattern of amoxicillin-clavulanic acid, carbenicillin, and ampicillin observed in this study resulted from the β-lactamase enzyme [55].…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial profile of Salmonella enterica serotype Choleraesuis from free-range swine in Kakamega fish market, western Kenya

Onyango

Ndeda

Wandili

et al. 2014

J Infect Dev Ctries

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Introduction: Salmonella enterica subspecies enterica serovar Choleraesuis is a host-adapted, facultative, intracellular pathogen that causes swine paratyphoid. Its antimicrobial resistance presents a challenge to feed manufacturing industries. However, stopping antibiotics in animal feed would have economic implications for the industry. Methodology: Conventional microbial methods for isolation and identification of S. Choleraesuis were employed. The isolates were subjected to screening against 17 antimicrobial agents and genotyping of resistance markers by PCR. The data were then analyzed and presented in percentages. Results: Phenotypically, 43 out of 95 isolates showed multidrug resistance. Among the 17 antibiotics tested, resistance was observed as follows: sulphonamides (45

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Prevalence and Characterization of Extended-Spectrum Beta-lactamases-ProducingSalmonella entericaIsolates in Saragossa, Spain (2001–2008)

Cited by 17 publications

References 35 publications

IncI1 Plasmids Carrying Various bla _CTX-M Genes Contribute to Ceftriaxone Resistance in Salmonella enterica Serovar Enteritidis in China

IncI1 Plasmids Carrying Various bla _CTX-M Genes Contribute to Ceftriaxone Resistance in Salmonella enterica Serovar Enteritidis in China

Dissemination of IncI2 Plasmids That Harbor the bla _CTX-M Element among Clinical Salmonella Isolates

Antimicrobial profile of Salmonella enterica serotype Choleraesuis from free-range swine in Kakamega fish market, western Kenya

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Prevalence and Characterization of Extended-Spectrum Beta-lactamases-ProducingSalmonella entericaIsolates in Saragossa, Spain (2001–2008)

Cited by 17 publications

References 35 publications

IncI1 Plasmids Carrying Various bla CTX-M Genes Contribute to Ceftriaxone Resistance in Salmonella enterica Serovar Enteritidis in China

IncI1 Plasmids Carrying Various bla CTX-M Genes Contribute to Ceftriaxone Resistance in Salmonella enterica Serovar Enteritidis in China

Dissemination of IncI2 Plasmids That Harbor the bla CTX-M Element among Clinical Salmonella Isolates

Antimicrobial profile of Salmonella enterica serotype Choleraesuis from free-range swine in Kakamega fish market, western Kenya

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Product

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IncI1 Plasmids Carrying Various bla _CTX-M Genes Contribute to Ceftriaxone Resistance in Salmonella enterica Serovar Enteritidis in China

IncI1 Plasmids Carrying Various bla _CTX-M Genes Contribute to Ceftriaxone Resistance in Salmonella enterica Serovar Enteritidis in China

Dissemination of IncI2 Plasmids That Harbor the bla _CTX-M Element among Clinical Salmonella Isolates