Prevalence and characterization of Salmonella infantis isolates originating from different points of the broiler chicken–human food chain in Hungary

Nógrády, Noémi; Kardos, Gábor; Bistyák, A.; Turcsányi, I.; Mészáros, János; GALANTAI, Z; Juhász, Alexandra; Samu, Péterné; KASZANYITZKY, J; Pászti, Judit

doi:10.1016/j.ijfoodmicro.2008.07.005

Cited by 80 publications

(55 citation statements)

References 20 publications

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“…In addition, the relatively frequent findings of other serovars of public health importance, such as S. Hadar, S. Virchow, and S. Kentucky on broiler meat indicate that broilers are a relevant reservoir for these serovars as well and constitute a potential (Nógrády et al, 2007;Nógrády et al, 2008).…”

Section: Relevance Of the Findings To Human Healthmentioning

confidence: 99%

Analysis of the baseline survey on the prevalence of Campylobacter in broiler batches and of Campylobacter and Salmonella on broiler carcasses in the EU, 2008 ‐ Part A: Campylobacter and Salmonella prevalence estimates

Publication¹

2010

EFS2

173

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A European Union‐wide baseline survey on Campylobacter in broiler batches and on Campylobacter and Salmonella on broiler carcasses was carried out in 2008. A total of 10,132 broiler batches were sampled from 561 slaughterhouses in 26 European Union Member States and two countries not belonging to the European Union. From each randomly selected batch the caecal contents of 10 slaughtered broilers were collected, pooled and examined for Campylobacter. From the same batch one carcass was collected after chilling and the neck skin together with the breast skin was examined for the presence of Campylobacter and Salmonella, in addition to the determination of the Campylobacter counts. Campylobacter was detected in pooled caecal contents of broilers and on broiler carcasses in all participating countries. At Community level the prevalence of Campylobacter‐colonised broiler batches was 71.2% and that of Campylobacter‐contaminated broiler carcasses was 75.8%. The Member State prevalence varied from 2.0% to 100.0% and from 4.9% to 100.0%, for caecal contents and carcasses, respectively. The results of the counts of Campylobacter on broiler carcasses showed substantial variation among the countries in contamination levels. About two‐thirds of the Campylobacter isolates from the pooled caecal contents as well as from the broiler carcasses were identified as Campylobacter jejuni, while one‐third was Campylobacter coli. Twenty‐two Member States and one non‐Member State isolated Salmonella on the broiler carcasses, with a Community prevalence of 15.6%. This prevalence varied widely among the Member States, from 0.0% to 26.6%. However, one Member State had an exceptionally high prevalence of 85.6% with the majority of isolates being S. Infantis. The Community prevalence of Salmonella Enteritidis or Salmonella Typhimurium‐contaminated broiler carcasses was 3.6%. Salmonella Infantis and Salmonella Enteritidis were the two most frequently isolated serovars on broiler carcasses in the EU and accounted for about one‐third and one‐sixth of the Salmonella isolates, respectively.

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Section: Relevance Of the Findings To Human Healthmentioning

confidence: 99%

Analysis of the baseline survey on the prevalence of Campylobacter in broiler batches and of Campylobacter and Salmonella on broiler carcasses in the EU, 2008 ‐ Part A: Campylobacter and Salmonella prevalence estimates

Publication¹

2010

EFS2

173

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“…Out of 64 isolates that were identifi ed as S. Infantis by conventional serotyping the same result was obtained for only 39 isolates (61 %) in simplex PCR. This was unexpected since selected primers were reported to be successfully used in S. Infantis identifi cation [18,[24][25][26][27]. The pair of primers used for identifi cation of S. Infantis serovar were designed to amplify practically the entire variable region of the fl agellar antigen fl jB gene.…”

Section: Discussionmentioning

confidence: 99%

Development of PCR-based identification of Salmonella enterica serovars

Kiskároly¹,

Morić

Đokić

et al. 2017

Acta Veterinaria

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The aim of the study was to evaluate and adapt the PCR-based protocol that utilizes the developed serotype-specifi c primers to identify Salmonella enterica species and its serotypes that are most frequently isolated from poultry samples in Vojvodina. Using the slide agglutination test, 64 and 33 out of 107 Salmonella isolates were identifi ed as S. Infantis and S. Enteritidis, respectively, while ten isolates were identifi ed as eight different Salmonella serovars. Using the same isolates, presence of 993-bp (bcfC gene), 636-bp (steB gene) and 293-bp (sdf locus) amplicons in multiplex PCR unambiguously identifi ed 31 isolates as S. Enteritidis. Two isolates identifi ed as Enteritidis in slide agglutination test were not identifi ed as such in PCR-based approach since they both were missing 293-bp long PCR product. Thirty-nine isolates produced a 727-bp amplicon in the specifi c simplex PCR, and thus were identifi ed as S. Infantis. The greatest discrepancy in comparison to the results of conventional serotyping has been observed in the case of S. Infantis, since 25 more isolates were noted as S. Infantis by conventional serotyping. Seven isolates, with unexpected PCR profi les stayed unidentifi ed by molecular typing, although they were serotyped as S. Typhimurium (1) and S. Infantis (6). S. Gallinarum serovar has to be additionally confi rmed, since it shares the same PCR profi le with S. Livingstone. Clearly, PCR-based identifi cation has to be thoroughly checked, verifi ed and adapted if it is to be applied as the routine identifi cation protocol.

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“…All statistical analyses were conducted using the software XLStat Version 2013.5.06 (AddinSoft, New York, NY, USA). compared to Sl1 and Sl2, suggesting poor hygienic conditions in chicken cages and the relevance of the intrinsic contamination in animals as the source of entry of microorganisms into slaughterhouses, as described by Nogrady et al (2008) and Tirolli & Costa (2006). However, other aspects of chicken production must be considered to explain such differences and not being considered in this study, such as the age of the birds, their body weights, the feed withdrawal and transport duration and the environment conditions: such aspects can interfere directly the initial microbial contamination of slaughtering facilities.…”

Section: Methodsmentioning

confidence: 95%

Self-Monitoring Microbiological Criteria for the Assessment of Hygienic Procedures During Chicken Slaughtering

Dias

Dianin

Bersot

et al. 2017

Rev. Bras. Cienc. Avic.

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Self-monitoring procedures are adopted by food industries to ensure the quality and safety of final products, considering hygiene and processing criteria. This study aimed to evaluate contamination in chicken processing, considering the microbiological criteria proposed by self-monitoring systems. Environmental samples from reception, slaughtering and processing were collected from three chicken slaughterhouses (Sl1, Sl2, Sl3), and subjected to microbiological analysis to enumerate hygiene indicators microorganisms: mesophilic aerobes, enterobacteriaceae, coliforms and Escherichia coli. The obtained counts were converted to log 10 , compared by ANOVA (p<0.05) and self-monitoring microbiological criteria for each slaughterhouse were considered. In reception, the mean counts of hygiene indicator microorganisms in Sl3 were significantly higher than mean counts observed in Sl1 and Sl2 (p<0.05). During slaughtering, the chilling was enough to decrease the mean counts of all hygiene indicator microorganisms in Sl1, Sl2 and Sl3 (p<0.05). Based on self-monitoring criteria, in the first stages of slaughtering the facilities presented higher frequencies of chicken carcasses with counts above their respective reference values. Sl02 presented carcasses with higher counts after final washing, resulting in environmental samples with higher counts when compared to Sl1 and Sl3 (p<0.05). Even considering the high counts observed in the initial steps of chicken processing and slaughtering, the results indicated the efficacy of hygienic procedures in providing chicken carcasses and cuts with low microbiological contamination. Self-monitoring criteria supported these results, and the high levels of microbial contamination during the initial steps of slaughtering require subsequent antimicrobial hygienic procedures.

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Prevalence and characterization of Salmonella infantis isolates originating from different points of the broiler chicken–human food chain in Hungary

Cited by 80 publications

References 20 publications

Analysis of the baseline survey on the prevalence of Campylobacter in broiler batches and of Campylobacter and Salmonella on broiler carcasses in the EU, 2008 ‐ Part A: Campylobacter and Salmonella prevalence estimates

Analysis of the baseline survey on the prevalence of Campylobacter in broiler batches and of Campylobacter and Salmonella on broiler carcasses in the EU, 2008 ‐ Part A: Campylobacter and Salmonella prevalence estimates

Development of PCR-based identification of Salmonella enterica serovars

Self-Monitoring Microbiological Criteria for the Assessment of Hygienic Procedures During Chicken Slaughtering

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