Prevalence and clinical aspects of human Trichostrongylus colubriformis infection in Lao PDR

Watthanakulpanich, Dorn; Pongvongsa, Tiengkham; Sanguankiat, Surapol; Nuamtanong, Supaporn; Maipanich, Wanna; Yoonuan, Tippayarat; Phuphisut, Orawan; Boupha, Boungnong; Moji, Kazuhiko; Sato, Megumi; Waikagul, Jitra

doi:10.1016/j.actatropica.2013.01.002

Cited by 22 publications

(32 citation statements)

References 13 publications

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“…This result is compatible with the result of a recent molecular study on human trichostrongyliasis in Mazandaran Province, in which T. colubriformis was accounted as the most probable common species of Trichostrongylus in humans [15]. Human trichostrongyliasis is caused by using animal feces as a fertilizer for agriculture and gardening [31]. In the north of Iran, many domestic animals, such as sheep, goats, and cows graze almost freely around, and contamination risk of vegetables in the fields with animal feces is high.…”

Section: Discussionsupporting

confidence: 89%

Molecular Phylogenetics of Trichostrongylus Species (Nematoda: Trichostrongylidae) from Humans of Mazandaran Province, Iran

et al. 2017

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The present study was performed to analyze molecularly the phylogenetic positions of human-infecting Trichostrongylus species in Mazandaran Province, Iran, which is an endemic area for trichostrongyliasis. DNA from 7 Trichostrongylus infected stool samples were extracted by using in-house (IH) method. PCR amplification of ITS2-rDNA region was performed, and products were sequenced. Phylogenetic analysis of the nucleotide sequence data was performed using MEGA 5.0 software. Six out of 7 isolates had high similarity with Trichostrongylus colubriformis, while the other one showed high homology with Trichostrongylus axei registered in GenBank reference sequences. Intra-specific variations within isolates of T. colubriformis and T. axei amounted to 0–1.8% and 0–0.6%, respectively. Trichostrongylus species obtained in the present study were in a cluster with the relevant reference sequences from previous studies. BLAST analysis indicated that there was 100% homology among all 6 ITS2 sequences of T. colubriformis in the present study and most previously registered sequences of T. colubriformis from human, sheep, and goat isolates from Iran and also human isolates from Laos, Thailand, and France. The ITS2 sequence of T. axei exhibited 99.4% homology with the human isolate of T. axei from Thailand, sheep isolates from New Zealand and Iran, and cattle isolate from USA.

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Section: Discussionsupporting

confidence: 89%

Molecular Phylogenetics of Trichostrongylus Species (Nematoda: Trichostrongylidae) from Humans of Mazandaran Province, Iran

et al. 2017

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“…In the recent study, the authors reported the occurrence of natural human infection by T. longispicularis for the first time in the world [6]. Also, T. colubriformis was predominant among Trichostrongylus species infecting humans in Thailand [38], France [39] and Laos [4,8]. This may be due to high prevalence of this species in herbivorous animals and also its high zoonotic potential [2].…”

Section: Discussionmentioning

confidence: 99%

“…Prevalences of 53. 6, 46.8, 42.4, 38.7, 37 and 35.8% have been reported in ruminants from Isfahan, Khuzestan, Mazandaran, Kermanshah, Hormozgan and West Azerbaijan provinces and 71, 67, 42,37,19,8,2 and 1% in humans in Khuzestan, Isfahan, Tehran, Hormozgan, Kermanshah, Mazandaran, West Azerbaijan and Sistan & Baluchestan provinces in the past decades respectively [2]. Recent studies indicate a decreasing trend in the prevalences of trichostrongylosis in both animals and humans in Iran.…”

Section: Introductionmentioning

confidence: 98%

“…Trichostrongylus species are primarily parasites of livestock with a worldwide distribution but they have also been reported from humans in Middle and Far East and Africa, particularly in rural communities with poor personal hygiene and close cohabitation with herbivorous animals [2,4,[6][7][8]. Epidemiological studies have demonstrated trichostrongylosis as a prevalent parasitic infection in domestic ruminants and humans in different regions of Iran [2,9].…”

Section: Introductionmentioning

confidence: 99%

“…Humans mostly acquire the infection through consumption of fresh vegetables contaminated with infective filariform larvae [6,7]. Trichostrongylosis is usually asymptomatic in low intensity infections, but abdominal pain, diarrhea, anorexia, nausea, weakness, mild anemia, low-grade peripheral eosinophilia, pulmonary and cutaneous symptoms are the most common manifestations in heavy infections [8,16,17].…”

Section: Introductionmentioning

confidence: 99%

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Zoonotic transmission of Teladorsagia circumcincta and Trichostrongylus species in Guilan province, northern Iran: molecular and morphological characterizations

et al. 2020

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Background: Parasitic trichostrongyloid nematodes have a worldwide distribution in ruminants and frequently have been reported from humans in Middle and Far East, particularly in rural communities with poor personal hygiene and close cohabitation with herbivorous animals. Different species of the genus Trichostrongylus are the most common trichostrongyloids in humans in endemic areas. Also, Ostertagia species are gastrointestinal nematodes that mainly infect cattle, sheep and goats and in rare occasion humans. The aim of the present study was to identify the trichostrongyloid nematodes obtained from a familial infection in Guilan province, northern Iran, using morphological and molecular criteria. Methods: After anthelmintic treatment, all fecal materials of the patients were collected up to 48 h and male adult worms were isolated. Morphological identification of the adult worms was performed using valid nematode keys. Genomic DNA was extracted from one male worm of each species. PCR amplification of ITS2-rDNA region was carried out, and products were sequenced. Phylogenetic analysis of the nucleotide sequence data was performed using MEGA 6.0 software. Results: Adult worms expelled from the patients were identified as T. colubriformis, T. vitrinus and Teladorsagia circumcincta based on morphological characteristics of the males. Phylogenetic analysis illustrated that each species obtained in current study was placed together with reference sequences submitted to GenBank database. Conclusions: The finding of current study confirms the zoonotic aspect of Trichostrongylus species and T. circumcincta in inhabitants of Guilan province. The occurrence of natural human infection by T. circumcincta is reported for the first time in Iran and the second time in the world.

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A systematic review and meta-analysis on the prevalence, distribution and nematode species diversity in small ruminants: a Nigerian perspective

Karshima

2020

J Parasit Dis

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Prevalence and clinical aspects of human Trichostrongylus colubriformis infection in Lao PDR

Cited by 22 publications

References 13 publications

Molecular Phylogenetics of Trichostrongylus Species (Nematoda: Trichostrongylidae) from Humans of Mazandaran Province, Iran

Molecular Phylogenetics of Trichostrongylus Species (Nematoda: Trichostrongylidae) from Humans of Mazandaran Province, Iran

Zoonotic transmission of Teladorsagia circumcincta and Trichostrongylus species in Guilan province, northern Iran: molecular and morphological characterizations

A systematic review and meta-analysis on the prevalence, distribution and nematode species diversity in small ruminants: a Nigerian perspective

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