PREVALENCE AND COMPARISONS OF FIVE DIFFERENT DIAGNOSTIC METHODS FORHELICOBACTER PYLORIIN DIABETIC PATIENTS

Abstract

Helicobacter pylori is now regarded as a major gastroduodenal pathogen that is etiologically linked with duodenal and gastric disease. It has been suggested recently as an important factor for nongastroenterologic conditions such as coronary heart disease and diabetes mellitus. In this study, we planned to investigate the prevalence of H. pylori in diabetic patients and to evaluate five different diagnostic tests. Group I consisted of 67 patients with type II diabetes mellitus and seventy-three aged-matched health people served as control in group II. Group I was divided in two subgroups with good (Group IA) and poor (Group IB) glycemic control. H. pylori was diagnosed by five different tests: 1) biopsy, 2) culture, 3) gram staining, 4) imprint cytology and 5) brushing cytology. The usefulness of each test for each group was statistically compared. There was a higher prevalence for H. pylori in diabetic patients. This study showed that two positive out of five tests was most reliable for predicting the H. pylori in diabetic and nondiabetic patients. In conclusion, the prevalence of H. pylori is high in diabetic patients. Peristaltic activity, and impaired nonspecific immunity must be evaluated as risk factors in diabetics. We recommend that the 'gold standard' should be regarded as two positive out of these five different tests.

Helicobacter pylori infection might be responsible for the interconnection between type 1 diabetes and autoimmune thyroiditis

“…The high prevalence of H. pylori infection in patients with diabetes is generally explained by reduced gastric motility and peristaltic activity which may promote H. pylori colonization [22], various chemical changes in gastric mucosa following non-enzymatic glycosylation of mucins or increased sialic acid [23] which may be involved as a receptor for H. pylori on the cell surface by promoting adhesion of H. pylori to gastric mucosa cells [24] and an impaired non-specific immunity observed in patients with diabetes [25]. …”

Touch cytology in diagnosing Helicobacter pylori: comparison of four staining methods

“…Although culture of the organism and histological examination have been used as gold standards, 24,25 long‐term growth of the organism in culture, human errors in interpretation of the biopsy results and cost‐effectiveness issues restricted wide use of these tests as reference examinations 25,26 . No single test, therefore, was used as the gold standard, the same approach as that used previously 10,18,19,27 . Thus, Hp status was defined as the gold standard.…”

The diagnostic value of endoscopy and Helicobacter pylori tests for peptic ulcer patients in late post-treatment setting

“…Several studies [22,23] have demonstrated the reliability of H. pylori tests used before treatment, while post-treatment testing is not yet adequately studied. However, in the case of long-lasting recurrent dyspepsia after H. pylori eradication therapy, endoscopy has been strongly recommended [4].…”