Touch cytology in diagnosing <i>Helicobacter pylori</i>: comparison of four staining methods

Hashemi, M R; Rahnavardi, Mohammad; Bikdeli, Bavand; Zahedani, Mohsen Dehghani; Iranmanesh, F.

doi:10.1111/j.1365-2303.2007.00417.x

Cited by 10 publications

(4 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…[20] Hashemi et al . [31] studied the diagnostic accuracy of four different staining methods on touch cytology, and stated that rapid urease test should still be acknowledged as the primary test for diagnosing H. pylori following upper gastrointestinal endoscopy.…”

Section: Discussionmentioning

confidence: 99%

Correlation of serology with morphological changes in gastric biopsy in Helicobacter pylori infection and evaluation of immunohistochemistry for H. pylori identification

Shukla

Agarwal

Pujani

et al. 2012

Saudi J Gastroenterol

View full text Add to dashboard Cite

Background/Aim:Helicobacter pylori is implicated in various gastroduodenal diseases and many tests are available for its detection. The present study attempted to document the morphological changes in the gastric mucosa induced by H. pylori colonization and correlate them with the severity of the infection. The study also compared various diagnostic tests and evaluated the different staining methods used for H. pylori detection, especially immunohistochemical identification.Patients and Methods:One hundred and two patients with dyspepsia were included. Enzyme-linked immunosorbent assay (ELISA) for H. pylori-specific immunoglobulin G (IgG), immunoglobulin A (IgA), and immunoglobulin M (IgM) was used. Rapid urease test was performed on endoscopic biopsy and it was stained with hematoxylin and eosin (H and E), modified Giemsa, and immunohistochemical stains.Results:A significant correlation was found between the density of H. pylori and severity of gastritis. A significant correlation was observed between serology (especially when used in combination, IgG and IgA) and status of H. pylori. Immunohistochemical staining enhanced the diagnostic yield of H. pylori detection.Conclusions:Immunohistochemistry (IHC) should be used judiciously, whereas simple and economical tests like modified Giemsa should be used routinely for the detection of H. pylori. Combined ELISA (IgG and IgA) should be preferred over single ELISA. Simultaneous morphological and serological detection of H. pylori is preferable as H. pylori may not be detected on morphology alone due to its patchy distribution in the stomach.

show abstract

Section: Discussionmentioning

confidence: 99%

Correlation of serology with morphological changes in gastric biopsy in Helicobacter pylori infection and evaluation of immunohistochemistry for H. pylori identification

Shukla

Agarwal

Pujani

et al. 2012

Saudi J Gastroenterol

View full text Add to dashboard Cite

show abstract

“…H . pylori status was determined by rapid Urease test and Giemsa staining methods [21], [22]. It was considered as H .…”

Section: Methodsmentioning

confidence: 99%

Circulating Methylated XAF1 DNA Indicates Poor Prognosis for Gastric Cancer

Ling

et al. 2013

PLoS ONE

View full text Add to dashboard Cite

BackgroundMethylated DNA in fluids may be a suitable biomarker for cancer patients. XAF1 has been shown to be frequently down-regulated in human gastric cancer (GC). Here, we investigated if XAF1 methylation in GC could be a useful biomarker.MethodsReal-time RT-PCR was used to detect XAF1 mRNA expression; immunohistochemistry and western blot were used to examine XAF1 protein expression in GC tissues (n = 202) and their corresponding para-cancerous histological normal tissues (PCHNTs). Real-time methylation specific-PCR was used to investigate XAF1 promoter methylation in the same panel of GC tissues, their PCHNTs and sera.ResultsWe confirmed frequent XAF1 down-regulation in both mRNA and protein levels in GC tissues as compared to normal controls and PCHNTs. XAF1 hypermethylation was evidenced in 83.2% (168/202) of GC tissues and 27.2% (55/202) of PCHNTs, while no methylation was detected in the 88 normal controls. The methylation level in GC tissues was significantly higher than that in PCHNTs (p<0.05). The hypermethylation of XAF1 significantly correlated with the down-regulation of XAF1 in GC tissues in both mRNA and protein levels (p<0.001 each). Moreover, we detected high frequency of XAF1 methylation (69.8%, 141 out of 202) in the sera DNAs from the same patients, while the sera DNAs from 88 non-tumor controls were negative for XAF1 methylation. The XAF1 methylation in both GC tissues and in the sera could be a good biomarker for diagnosis of GC (AUC = 0.85 for tissue and AUC = 0.91 for sera) and significantly correlated with poorer prognosis (p<0.001). In addition, after-surgery negative-to-positive transition of XAF1 methylation in sera strongly associated with tumor recurrence.Conclusions1) Dysfunction of XAF1 is frequent and is regulated through XAF1 promoter hypermethylation; 2) Detection of circulating methylated XAF1 DNAs in the serum may be a useful biomarker in diagnosis, evaluating patient’s outcome (prognosis and recurrence) for GC patients.

show abstract

“…[ 9 ] Gastric microbiota (cocci, bacilli, hyphae, and spores) was microscopically evaluated on the same smears prepared for cytologic examination and stained according to the Papanicolaou method [Figures 2 and 3 ]. [ 26 ] Helicobacter pylori ( H. pylori ) status was further examined in those GL samples showing bacilli by our bacteriology laboratory technicians; bacterial features such as Gram-negative staining, helical or spiral shape, flagellar filaments, diameter of about 0.5 μm, and positive correlation with preoperative gastric biopsies were considered consistent with the microbiologic diagnosis of H. pylori infection. Histopathology of surgical specimens was described following the 8 th ed.ition of AJCC TNM Staging System.…”

Section: Aterial and M Ethodsmentioning

confidence: 99%

Prognostic Role of Intragastric Cytopathology and Microbiota in Surgical Patients with Stomach Cancer

Virgilio

Giarnieri²,

Carico³

et al. 2021

Journal of Cytology

View full text Add to dashboard Cite

Background: In the last decade, analysis of malignant cells and flora in gastric lavage (GL) has provided interesting data on pathogenesis of gastric cancer (GC). For this study, combining such two aspects into one cyto-microbiologic category, we tested the prognostic role of the presence/absence of cancer cells (GL1/GL0) and bacterial microbiota (MB1/MB0) in our GC population. Material and Methods: Between April 2012 and August 2019, 79 surgical patients with GC were prospectively investigated with the determination of GL MB. Results: Compared with GL1 MB0, GL1 MB1 strongly correlated with advanced GC, portended poorer overall survival (OS) (45.8 months vs 20.5 months, P = 0.049), and resulted a significant ( P = 0.008) and an independent ( P = 0.013) prognostic factor unfavorable for OS. Conclusion: In the light of our results, the cyto-microbiologic parameter of GL MB should be used to gain a better prognosis of GC patients. Administration of antimicrobial treatment for MB1 subjects should be entertained because it could reduce the risk of oncogenesis.

show abstract

Touch cytology in diagnosing Helicobacter pylori: comparison of four staining methods

Cited by 10 publications

References 24 publications

Correlation of serology with morphological changes in gastric biopsy in Helicobacter pylori infection and evaluation of immunohistochemistry for H. pylori identification

Correlation of serology with morphological changes in gastric biopsy in Helicobacter pylori infection and evaluation of immunohistochemistry for H. pylori identification

Circulating Methylated XAF1 DNA Indicates Poor Prognosis for Gastric Cancer

Prognostic Role of Intragastric Cytopathology and Microbiota in Surgical Patients with Stomach Cancer

Contact Info

Product

Resources

About