Prevalence and detection of antibiotic-resistant determinant in Salmonella isolated from food-producing animals

Abstract

Salmonella spp. infections are considered as the most common food-borne disease globally. The contamination of food products with Salmonella has given rise to severe health and economic challenges. This study assessed the prevalence of Salmonella in the faeces of cows and goats in the Eastern Cape province of South Africa, their antibiotic resistance patterns as well as antibiotic-resistant gene determinant. Antibiotic disc was used for antibiogram profiles while polymerase chain reaction was employed for the detection of antibiotic-resistant genes. A total of 150 Salmonella were isolated from the faecal samples. Eighty two (55%) isolates were recovered from cow faeces while 68 (45%) were isolated from goat faeces. All Salmonella isolates were sensitive to ciprofloxacin (100%) while 95% were sensitive to ofloxacin. Also, a high sensitivity of 93 and 89% was observed against nalidixic acid and ofloxacin, respectively. Salmonella isolates demonstrated moderate sensitivity against cephalothin (70%), chloramphenicol (75%) and minocycline (68%) while 49% were resistant to tetracycline and erythromycin. The prevalence of the antibiotic-resistant genes in Salmonella isolates were detected as follows: integron conserved segment 28% (42/150), bla TEM gene 19.3% (29/150), blapse₁ 7.3% (11/150) and blaampC 4.7% (7/150). The results obtained in the study imply that cow and goat faeces could be potential reservoirs of Salmonella and could possibly cause infections as a result of contamination of food products. There is a need for a surveillance system to track resistance patterns of Salmonella circulating in South Africa.

Characterization of Integrons and Resistance Genes in Salmonella Isolates from Farm Animals in Shandong Province, China

“…In this study, a total of three β-lactamase genes were detected among the Salmonella isolates recovered from chickens, ducks and pigs: bla TEM−1 , bla PSE−1 , and bla CTX−M−55 . Most of the isolates carried bla TEM−1 , which was similar to the report in South Africa that bla TEM−1 was the most commonly identified β-lactamase gene in Salmonella isolates from food-producing animals (Igbinosa, 2015). In addition, in this study, most isolates carried bla TEM−1 and bla CTX−M−55 , which confer resistance to ampicillin.…”

Detection of antibiotic resistance, virulence gene determinants and biofilm formation in Aeromonas species isolated from cattle

“…The presence of potentially pathogenic bacteria in the beef could be a source of contamination of the beef product. Although faecal samples were assayed, however, in meat production, the eviscerations process the main route of contamination of meat and meat product by faecal bacteria (Stevens et al 2006;Vandendriessche, 2008;Igbinosa, 2015). In this study, Aeromonas species were isolated from cow faecal samples collected from Lovedale and Fort Cox farms.…”

Carriage and antimicrobial resistance of non-typhoidal <i>Salmonella</i> in cattle slaughtered in Ambo municipality abattoir, West Shewa zone, Oromia, Ethiopia - a point prevalence survey

“…However, the current prevalence is slightly lower than the work of Sibhat et al (2009) who reported 8% and 6% from mesenteric lymph nodes and caecal content, respectively. Very high prevalence of NTS (54.7%) was reported from cows' feces in South Africa (Igbinosa, 2015). The discrepancies in prevalence between different studies could be due to the difference in climate, season, age of animals, diet, management of animals, population density, degree of exposure of animals to stress factors like transportation and starvation, and actual difference in prevalence of NTS in cattle environments (Radostits et al, 2007).…”