Prevalence and disease associations in feline thrombocytopenia: a retrospective study of 194 cases

Ellis, Jenny; Bell, Robert; Barnes, Dain; Miller, Rachel G.

doi:10.1111/jsap.12814

Cited by 22 publications

(20 citation statements)

References 18 publications

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“…Clinical signs depend on viral subgroup and the stage of disease. Common general clinical signs include anemia (pale gums), hyporexia, decreased stamina, depression, diarrhea or constipation, excessive drinking and urination, infertility, jaundice, fever, lymphadenopathy, weight loss, poor coat condition, and neuropathies, with subsequent anisocoria, and hind limb paralysis [11].…”

Section: Introductionmentioning

confidence: 99%

Comparative measurement of FeLV load in hemolymphatic tissues of cats with hematologic cytopenias

et al. 2019

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BackgroundFeline leukemia virus (FeLV) is a serious viral infection in cats. FeLV is found in some tissues, such as spleen, lymph nodes and epithelial tissues. However, there is controversy about the organ in which the virus can be reliably detected in infected cats. The purpose of this study was to determine the level of viral infection in hemolymphatic tissues, including blood, bone marrow and spleen by reverse-transcriptase quantitative polymerase chain reaction (RT-qPCR).ResultsA total of 31 cats with clinical signs of FeLV infection associated with at least a single lineage hematologic cytopenia were included in this study. Peripheral blood, bone marrow and spleen samples were obtained from each cat. Complete blood counts, biochemical tests, and a rapid test to detect FeLV p27 antigen in blood samples of cats were performed. Of 31 cats, 9 had anemia alone, 4 had thrombocytopenia alone, 2 had neutropenia alone, 9 had bicytopenia of anemia and thrombocytopenia, 3 had bicytopenia of anemia and neutropenia, and 4 had pancytopenia. FeLV RNA was then detected by RT-qPCR in the whole blood, bone marrow and spleen. Viral RNA copy numbers were detected in all cats by RT-qPCR whereas 24 out of 31 cats were positive for the serum FeLV antigen. We detected a significantly greater number of viral RNA in the spleen compared with the whole blood and bone marrow.ConclusionSpleen is a site where FeLV is most frequently detected in cats with hematologic cytopenias.

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Section: Introductionmentioning

confidence: 99%

Comparative measurement of FeLV load in hemolymphatic tissues of cats with hematologic cytopenias

et al. 2019

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“…PLT aggregation in feline blood samples is common, with reported prevalence ranging between 40% and 70% ( 21 , 57 – 60 ). In this study, PLT aggregation occurred in 27% of the WB samples and in 7% of the PRP samples, with no evidence of PLT aggregation in the PPP samples.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of a Standardized Protocol for Plasma Rich in Growth Factors Obtention in Cats: A Prospective Study

et al. 2022

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IntroductionPlatelet-rich plasma (PRP) is an autologous plasma with platelet (PLT) concentration above that of whole blood (WB). PLTs contain growth factors (GFs) that promote tissular repair.ObjectivesTo determine and compare the concentrations of PLT, red blood cells (RBC) and white blood cells (WBC) between WB samples, PRP and platelet poor plasma (PPP) samples; and to analyze the concentrations of platelet-derived growth factor BB (PDGF-BB) and transforming growth factor β1 (TGF-β1) in the PRP and PPP of healthy adult cats using a standardized protocol with PRGF®-Endoret® characteristics.Material and MethodsWB was collected from 30 cats. PRP was obtained following three centrifugation protocols using PRGF®-Endoret® technology: 255, 260, and 265 g for 10 min each. The cellular components, RBC, WBC, PLT, and the concentrations of PDGF-BB and TGF-β1 in the PRP and PPP fractions were determined for each protocol.ResultsPLTs in the PRP fraction were statistically higher than WB, with no statistical differences between PPP and WB. In PRP fraction, PLT concentration was increased 1.4 times on average at 255 g; 1.3 times at 260 g and, 1.5 times at 265 g without statistical differences among them. The mean platelet volume (MPV) was significantly higher in WB compared to PRP and PPP fractions without significant differences between protocols. Compared to WB, the number of RBCs and WBCs was reduced by 99% and by more than 95% in PRP and PPP respectively, without significant differences between protocols. PDGF-BB concentrations were statistically higher in PRP than in PPP fractions, however, TGF-ß1 concentrations did not vary between fractions at 260 g. Comparing the three protocols within PRP and PPP fractions, no differences in PDGF-BB and TGF-ß1 concentrations were observed.Clinical RelevanceThe study shows scientific evidence regarding the obtention of PRP in cats using the PRGF®-Endoret® technology for the quantification of PDGF-BB and TGF-ß1. At 265 g for 10 min, PLT concentration was increased 1.5 times with unnoticeable erythrocytes and leukocytes in the samples. These results clearly show that the PRGF®-Endoret® methodology is suitable to obtain PRP in cats. Further studies are needed to determine the clinical efficacy of the obtained PGRF in the treatment of different pathologies in cats.

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“…Three cats with cardiac diseases also presented hemostatic disorders, including aortic or pulmonary thromboembolism, hemothorax, and disseminated intravascular coagulation [42]. A recent retrospective study performed at a UK hospital aiming to assess the prevalence of thrombocytopenia in 194 cats showed in subjects with cardiovascular disease a prevalence of 5% vs. 4% of cats with normal platelet count (P not significant) [43]. Conversely, a study on thrombocytosis observed this finding in 5 out of 51 cases of cardiovascular diseases: HCM, ATE, hypertension and thromboembolic disease.…”

Section: Coagulative Profilementioning

confidence: 99%

Updates on Laboratory Evaluation of Feline Cardiac Diseases

Gavazza

Marchegiani

Guerriero

et al. 2021

Veterinary Sciences

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Laboratory tests can be altered in cardiovascular diseases and the investigation of specific tests or biomarkers may provide additional information about myocardial damage. Traditional laboratory tests, such as cell blood count, serum biochemistry, and coagulation, can be useful in investigating patients, but are not specific. However, markers like Troponin and Natriuretic Peptides may possibly furnish further data on myocardium damage and can be used in both studying and monitoring cats with cardiac disease. Moreover, the evaluation of the thyroid profile is very important as hyperthyroid cats concomitant cardiovascular diseases are very common and they can also be a direct consequence of endocrinopathy. The purpose of this manuscript is to provide the widest possible overview of what is present in the literature about the feline clinical pathology of heart diseases through a rational division of the main alterations of traditional tests and biomarkers.

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Prevalence and disease associations in feline thrombocytopenia: a retrospective study of 194 cases

Cited by 22 publications

References 18 publications

Comparative measurement of FeLV load in hemolymphatic tissues of cats with hematologic cytopenias

Comparative measurement of FeLV load in hemolymphatic tissues of cats with hematologic cytopenias

Evaluation of a Standardized Protocol for Plasma Rich in Growth Factors Obtention in Cats: A Prospective Study

Updates on Laboratory Evaluation of Feline Cardiac Diseases

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