Prevalence and Distribution of Dengue Virus in Aedes aegypti in Yogyakarta City before Deployment of Wolbachia Infected Aedes aegypti

Rahayu, Ayu; Saraswati, Utari; Supriyati, Endah; Kumalawati, Dian Aruni; Hermantara, Rio; Rovik, Anwar; Daniwijaya, Edwin Widyanto; Fitriana, Iva; Setyawan, Sigit; Ahmad, Riris Andono; Wardana, Dwi Satria; Indriani, Citra; Tantowijoyo, Warsito; Arguni, Eggi

doi:10.3390/ijerph16101742

Cited by 27 publications

(18 citation statements)

References 41 publications

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“…Many viruses in this study were predominantly ISVs except for BAV, and no arboviruses, such as JEV and DENV, were isolated. Previous studies reported that DENV genomes were detected by RT-PCR in 0.12% (36/29,252) [ 47 ] and 0.66% (110/16,605) [ 41 ] in mosquito population. In a DENV-endemic area in Brazil, the MIR of DENV by RT-PCR in wild Aedes mosquitoes was 19.8 [ 37 ].…”

Section: Discussionmentioning

confidence: 99%

Mosquito-borne viruses, insect-specific flaviviruses (family Flaviviridae, genus Flavivirus), Banna virus (family Reoviridae, genus Seadornavirus), Bogor virus (unassigned member of family Permutotetraviridae), and alphamesoniviruses 2 and 3 (family Mesoniviridae, genus Alphamesonivirus) isolated from Indonesian mosquitoes

et al. 2020

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Mosquitoes transmit many kinds of arboviruses (arthropod-borne viruses), and numerous arboviral diseases have become serious problems in Indonesia. In this study, we conducted surveillance of mosquito-borne viruses at several sites in Indonesia during 2016–2018 for risk assessment of arbovirus infection and analysis of virus biodiversity in mosquito populations. We collected 10,015 mosquitoes comprising at least 11 species from 4 genera. Major collected mosquito species were Culex quinquefasciatus , Aedes albopictus , Culex tritaeniorhynchus , Aedes aegypti , and Armigeres subalbatus . The collected mosquitoes were divided into 285 pools and used for virus isolation using two mammalian cell lines, Vero and BHK-21, and one mosquito cell line, C6/36. Seventy-two pools showed clear cytopathic effects only in C6/36 cells. Using RT-PCR and next-generation sequencing approaches, these isolates were identified as insect flaviviruses (family Flaviviridae , genus Flavivirus ), Banna virus (family Reoviridae , genus Seadornavirus ), new permutotetravirus (designed as Bogor virus) (family Permutotetraviridae, genus Alphapermutotetravirus ), and alphamesoniviruses 2 and 3 ( family Mesoniviridae , genus Alphamesonivirus ). We believed that this large surveillance of mosquitoes and mosquito-borne viruses provides basic information for the prevention and control of emerging and re-emerging arboviral diseases.

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Section: Discussionmentioning

confidence: 99%

Mosquito-borne viruses, insect-specific flaviviruses (family Flaviviridae, genus Flavivirus), Banna virus (family Reoviridae, genus Seadornavirus), Bogor virus (unassigned member of family Permutotetraviridae), and alphamesoniviruses 2 and 3 (family Mesoniviridae, genus Alphamesonivirus) isolated from Indonesian mosquitoes

et al. 2020

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“…aegypti females collected in periods of high and low arbovirus transmission and tested by RT-PCR [ 30 ] led to estimates of DENV natural infection rate of <1% [ 30 ]. A similarly low DENV natural infection rate from individual mosquitoes was quantified both in Yogyakarta, Indonesia [ 61 ] and, in Ho Chi Minh City, Vietnam [ 62 ]. Particularly for DENV, our study found a similarly low natural infection rate (19/2,161 = 0.87%), in agreement with previous reports and consistent with the low number of reported dengue cases in the city ( S1 Fig ).…”

Section: Discussionmentioning

confidence: 99%

Natural arbovirus infection rate and detectability of indoor female Aedes aegypti from Mérida, Yucatán, Mexico

et al. 2021

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Arbovirus infection in Aedes aegypti has historically been quantified from a sample of the adult population by pooling collected mosquitoes to increase detectability. However, there is a significant knowledge gap about the magnitude of natural arbovirus infection within areas of active transmission, as well as the sensitivity of detection of such an approach. We used indoor Ae. aegypti sequential sampling with Prokopack aspirators to collect all mosquitoes inside 200 houses with suspected active ABV transmission from the city of Mérida, Mexico, and tested all collected specimens by RT-PCR to quantify: a) the absolute arbovirus infection rate in individually tested Ae. aegypti females; b) the sensitivity of using Prokopack aspirators in detecting ABV-infected mosquitoes; and c) the sensitivity of entomological inoculation rate (EIR) and vectorial capacity (VC), two measures ABV transmission potential, to different estimates of indoor Ae. aegypti abundance. The total number of Ae. aegypti (total catch, the sum of all Ae. aegypti across all collection intervals) as well as the number on the first 10-min of collection (sample, equivalent to a routine adult aspiration session) were calculated. We individually tested by RT-PCR 2,161 Aedes aegypti females and found that 7.7% of them were positive to any ABV. Most infections were CHIKV (77.7%), followed by DENV (11.4%) and ZIKV (9.0%). The distribution of infected Aedes aegypti was overdispersed; 33% houses contributed 81% of the infected mosquitoes. A significant association between ABV infection and Ae. aegypti total catch indoors was found (binomial GLMM, Odds Ratio > 1). A 10-min indoor Prokopack collection led to a low sensitivity of detecting ABV infection (16.3% for detecting infected mosquitoes and 23.4% for detecting infected houses). When averaged across all infested houses, mean EIR ranged between 0.04 and 0.06 infective bites per person per day, and mean VC was 0.6 infectious vectors generated from a population feeding on a single infected host per house/day. Both measures were significantly and positively associated with Ae. aegypti total catch indoors. Our findings provide evidence that the accurate estimation and quantification of arbovirus infection rate and transmission risk is a function of the sampling effort, the local abundance of Aedes aegypti and the intensity of arbovirus circulation.

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“…DENV-1 was the arbovirus with the highest frequency in the population of infected individuals at 82% (50/61) and the overall infection rate in A. aegypti and A. albopictus was 29.6% (50/169) ( Table 2). The DENV-1 serotype has also been detected more frequently than other arboviruses in Indonesia, Mexico, Cuba, Brazil, and Colombia (Velandia-Romero et al, 2017;Eiras et al, 2018;Garcia-Rejon et al, 2018;Gutiérrez-Bugallo et al, 2018;Rahayu et al, 2019). The high frequency of this arbovirus may be because the transmission in the urban cycle of DENV is provided by the level of viremia of the hosts (humans) since individuals at the beginning of infection have high rates of viremia (Duong et al, 2015;Martínez-Vega et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

Identification of Aedes (Diptera: Culicidae) Species and Arboviruses Circulating in Arauca, Eastern Colombia

et al. 2020

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The identification of vector species and their natural infection with arboviruses results in important data for the control of their transmission. However, for the eastern region of Colombia, this information is limited. Therefore, this study morphologically and molecularly identified species of the genus Aedes and the detection of arboviruses (Dengue, Chikungunya, Zika, and Mayaro) in female mosquitoes (individually) present in three municipalities (Saravena, Arauquita, and Tame) by amplifying the genetic material using RT-PCR (reverse transcriptase polymerase chain reaction) in the department of Arauca, eastern Colombia. Inconsistencies between morphological and molecular identification were detected in 13 individuals with Aedes albopictus initially determined as Aedes aegypti based on morphology (n = 13). Molecular identification showed the simultaneous presence of A. aegypti (n = 111) and A. albopictus (n = 58) in the urban municipalities of Saravena and Arauquita. These individuals were naturally infected with Dengue virus type 1 (DENV-1) and Chikungunya virus (CHIKV). The most frequent arbovirus was DENV-1 with an infection rate of 40.7% (11/27) for A. aegypti and 39.7% (23/58) for A. albopictus, which was followed by CHIKV with an infection rate of 1.8% for A. aegypti (2/111) and 6.9% for A. albopictus (4/58). Additionally, a mixed infection of DENV-1 and CHIKV was obtained in 4.5% of A. aegypti (5/111). Zika virus (ZIKV) and Mayaro virus (MAYV) infections were not detected. This study found that barcoding (fragment gene COI) is a successful method for identifying Aedes species. Additionally, we recommend the individual processing of insects as a more accurate strategy for arboviruses detection since the infection rate is obtained and co-infection between DENV-1 and CHIKV is also possible.

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Prevalence and Distribution of Dengue Virus in Aedes aegypti in Yogyakarta City before Deployment of Wolbachia Infected Aedes aegypti

Cited by 27 publications

References 41 publications

Natural arbovirus infection rate and detectability of indoor female Aedes aegypti from Mérida, Yucatán, Mexico

Identification of Aedes (Diptera: Culicidae) Species and Arboviruses Circulating in Arauca, Eastern Colombia

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