Prevalence and distribution of indoor allergens in Singapore

Zhang, L.; Chew, Fook Tim; Soh, S. Y.; Yi, Fong Cheng; Law, S. Y.; Goh, Daniel Y T; Lee, B. W.

doi:10.1111/j.1365-2222.1997.tb01227.x

Cited by 57 publications

(62 citation statements)

References 33 publications

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“…Another observation from the present study worth noting is that the sensitization to Der p 2 was below 50% in both Singaporean and Malaysian subjects. This is rather surprising, as a previous epidemiological study indicated that an extremely high number of both Der p and Blo t mites were found in Singaporean homes [6], and the levels of Blo t and Der p allergens found in these homes were very high [27]. This result also shows a discrepancy with other published studies in which it was reported that about 80% of mite-allergic asthmatic patients were sensitized to Der p 2 [28, 29, 30].…”

Section: Discussionmentioning

confidence: 85%

Sensitization Profiles of Malaysian and Singaporean Subjects to Allergens from Dermatophagoides pteronyssinus and Blomia tropicalis

Yeoh¹,

Kuo²,

Wang

et al. 2003

Int Arch Allergy Immunol

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Background: The house dust mites Dermatophagoides pteronyssinus (Der p) and Blomia tropicalis (Blo t) are the most common house dust mite species in Southeast Asia. To date, there have only been a few studies on the sensitization profile of the general populations in Southeast Asia to house dust mites. The aim of this study was to determine the profiles of Der p and Blo t sensitization among Singaporean and Malaysian subjects. Methods: Enzyme-linked immunosorbent assay was used to detect specific IgE to Der p and Blo t mite crude extracts as well as purified Der p 1, Der p 2 and Blo t 5 allergens. Sera used were from 229 Singaporean subjects (124 with rhinitis, 105 without rhinitis) and 143 Malaysian subjects (94 adults and 49 children with asthma). Results: The sensitization profile of rhinitis subjects to the dust mite allergens used in this study was as follows: Blo t extract positive: 91/124 (73%); Blo t 5 positive: 62/124 (50%); Der p extract positive: 61/124 (49%); Der p 1 positive: 53/124 (43%); Der p 2 positive: 45/124 (36%). The nonrhinitis subjects’ sensitization profile was as follows: Blo t extract positive: 60/105 (57%); Blo t 5 positive: 24/105 (23%); Der p extract positive: 38/105 (36%); Der p 1 positive: 14/105 (13%); Der p 2 positive: 17/105 (16%). The study of Malaysian asthmatic adults showed that 39% of them were sensitized to Der p 1, 32% to Der p 2 and 37% to Blo t 5. Among the asthmatic children, sensitization to Blo t 5, Der p 1 and Der p 2 was 90, 57 and 39%, respectively. Conclusion: This study clearly revealed that dual sensitization to B. tropicalis and D. pteronyssinus is common in the general populations of Singapore and Malaysia. Sensitization to Blo t 5 is more prevalent than to Der p 1 and Der p 2.

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Section: Discussionmentioning

confidence: 85%

Sensitization Profiles of Malaysian and Singaporean Subjects to Allergens from Dermatophagoides pteronyssinus and Blomia tropicalis

Yeoh¹,

Kuo²,

Wang

et al. 2003

Int Arch Allergy Immunol

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“…Dust mites are distributed worldwide but their prevalence varies from one region to the next [3]. Blomia tropicalis (Bt) is the most predominant mite species in tropical countries [4, 5]. …”

Section: Introductionmentioning

confidence: 99%

cDNA Cloning and Expression of Blo t 11, the Blomia tropicalis Allergen Homologous to Paramyosin

Ramos¹,

Cheong

Lee³

et al. 2001

Int Arch Allergy Immunol

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Blomia tropicalis is an important mite species in many parts of the world and the most predominant mite species in tropical countries. The prevalence of sensitization to this species has probably been underestimated because commercial extracts are largely unavailable. Identification and characterization of B. tropicalis allergens is an important step toward understanding the role of this species in allergic sensitization and could provide appropriate reagents for diagnostic and therapeutic procedures. This paper describes the isolation, sequence analysis, expression and allergenicity of a cDNA gene coding for a B. tropicalis allergen with homology to paramyosin, a high-molecular-weight allergen previously identified in Dermatophagoides farinae. The full-length Blo t 11 cDNA gene was isolated by cDNA library screening, 5′-rapid amplification of cDNA ends and long-distance PCR. Sequence analysis was performed with a combination of CLUSTAL W, CGC and BLAST program packages. The cDNA gene was expressed as a GST fusion protein in Escherichia coli and purified by affinity chromatography using the glutathione Sepharose column. Allergenicity of the rBlo t 11 was tested by human IgE dot blot immunoassay. Blo t 11 is a 3,111-bp cDNA gene with a 2,625-bp open reading frame coding for an 875-amino acid protein, exhibiting significant homology with different invertebrate paramyosins. The human IgE dot blot immunoassay showed that the rBlo t 11 reacted positively to 52% (33/63) of sera from asthmatic patients. Blo t 11 is the homolog of Der f 11 exhibiting potentially important allergenic activity.

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“…Similar results were reported from different areas around the world. Zhang and others 30 reported that B. tropicalis allergens were detected in 494/590 (84%) of dust samples tested in Singapore. They claim that B. tropicalis is the most prevalent mite identified in house dust in Singapore and the atopic population is highly sensitized to B. tropicalis allergens.…”

Section: Discussionmentioning

confidence: 99%

Quantitation of Blomia tropicalis Allergen Blo t 5 in Cereal and Cereal-Based Foods Consumed in the Nile Delta, Egypt

Hussein

Elawamy

2015

The American Society of Tropical Medicine and Hygiene

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Abstract. The mite Blomia tropicalis has significant prevalence worldwide. Blo t 5 is a major B. tropicalis allergen that has been associated with sensitization and allergic symptoms in many asthmatic patients. Besides house dust, contaminated foodstuffs are an important source of exposure to B. tropicalis allergens. In this study, a double sandwich enzyme-linked immunosorbent assay (ELISA) using mAb 4D4 and biotinylated mAb 4G9 was done to detect Blo t 5 allergen in different types of raw and processed foods, including wheat, corn, rice, bean, wheat and corn flour, cake, and rusk, which were collected from retail stores in the Nile Delta of Egypt. Out of 88 samples, 38 (43.2%) were positive for Blo t 5 allergen with no significant statistical difference in positivity according to food type. In positive samples, the Blo t 5 concentration ranged from 10 ng/g to 790 ng/g. This study showed that Blo t 5 should be considered as an important allergen that presents a risk to the Egyptian population and should become a routine allergen in the skin prick test to improve the management of allergic disease.

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Prevalence and distribution of indoor allergens in Singapore

Abstract: The results indicate that compared with public places, the home consitutes a major reservior of indoor allergens. Allergens of the storage mite, B. tropicalis, should be considered as a major allergenic component of dust in Singapore.

Cited by 57 publications

References 33 publications

Sensitization Profiles of Malaysian and Singaporean Subjects to Allergens from <i>Dermatophagoides pteronyssinus</i> and <i>Blomia tropicalis</i>

Sensitization Profiles of Malaysian and Singaporean Subjects to Allergens from <i>Dermatophagoides pteronyssinus</i> and <i>Blomia tropicalis</i>

cDNA Cloning and Expression of Blo t 11, the <i>Blomia tropicalis</i> Allergen Homologous to Paramyosin

Quantitation of Blomia tropicalis Allergen Blo t 5 in Cereal and Cereal-Based Foods Consumed in the Nile Delta, Egypt

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