Prevalence and etiology of buffalo mastitis and milk somatic cell count in dry and rainy seasons in a buffalo herd from Analândia, São Paulo State, Brazil

Abstract

The aim of this study was to evaluate somatic cell count (SCC), prevalence and etiology of mastitis in a dairy buffalo herd from Analândia, São Paulo State, Brazil, in the dry and rainy seasons. Additionally, antimicrobial susceptibility profile of microorganisms isolated from milk samples was also evaluated. 1,042 milk samples from female Murrah buffaloes in a dairy farm located in Analândia, São Paulo State, Brazil, collected between May 2011 and November 2012 were analyzed. After the mammary gland physical examination, strip cup test and California Mastitis Test (CMT) were performed. Afterwards, 50mL of milk samples from each mammary quarter were collected aseptically for SCC in automatic equipment and microbiological examination. The antimicrobial sensitivity profile to ampicillin, cefoperazone, ceftiofur, enrofloxacin, gentamicin, neomycin, oxacillin, penicillin, and sulfamethoxazole/trimethoprim was evaluated by disk diffusion method. The monthly average temperature and pluviometric index were obtained from "Centro Integrado de Informações Agrometeorológicas" (CIIAGRO) of "Instituto Agronômico de Campinas" (IAC). Milk samples with positive results in the microbiological test showed average SCC of 137,720 cells/mL in the dry period and 190,309 cells/mL in the rainy period. Although a higher number of isolated microorganisms was observed in buffalo milk samples during the rainy period (69/600) compared to the dry period (50/442), the season had no significant effect on the frequency of isolation of microorganisms.

QUALITY OF REFRIGERATED RAW MILK FROM BUFFALO COWS (Bubalus bubalis bubalis) IN DIFFERENT FARMS AND SEASONS IN BRAZIL

“…Since the samples analyzed in the present study found a median value of 114.78x10 3 cells/mL, Brazilian buffalo dairy producers are adhering to the present legislation, although values outside the reference range have been found (Table 2). Pizauro et al (26) studied the prevalence and etiology of buffalo mastitis and milk somatic cell count during the dry and rainy seasons in a buffalo herd in the state of São Paulo, Brazil. They observed that, regarding the SCC count, milk samples with acceptable results in microbiological tests showed average values of 99.51x10 3 cells/mL and 125.6x10 3 cells/mL during cold and warm periods, respectively.…”

Species identification and antimicrobial susceptibility profile of bacteria causing subclinical mastitis in buffalo

“…In that study, the Staphylococcus genus was considered the main etiological agent, being of great epidemiological importance in buffalo mastitis. Indeed, the genus Staphylococcus has been predominantly isolated in samples from buffalo Murrah crossbred with subclinical mastitis (Dhakal et al 2008) and clinical mastitis (Pizauro et al 2014). Khan and Muhammad (2005) prevalence of S. epidermidis (30.1%) and S. aureus (4.8%) in buffaloes with mastitis in São Paulo, Brazil.…”

Mastitis in buffalo herds and their susceptibility to antimicrobial

“…Medeiros et al (2011) cel/mL com mediana de 328.000 cel/mL, levando a conclusão que valores de CCS acima de 280.000 cel/mL é um indicativo de infecção da glândula mamária. Pizauro et al (2014) estudaram amostras de leite com resultado positivo em exames microbiológicos, as quais apresentaram CCS média de 137.720 células/mL, no período seco, e 190.309 células/mL, no período chuvoso. As variações na CCS de amostras de leite com resultados positivos em testes microbiológicos recolhidos durante os períodos de seca e chuvosa pode estar relacionada com a fase de infecção e do tipo do isolado microrganismo.…”